PMID- 27905510
OWN - NLM
STAT- MEDLINE
DCOM- 20180604
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Dec 1
TI  - Remarkable alterations of Nav1.6 in reactive astrogliosis during epileptogenesis.
PG  - 38108
LID - 10.1038/srep38108 [doi]
LID - 38108
AB  - Voltage-gated sodium channels (VGSCs) play a vital role in controlling neuronal 
      excitability. Nav1.6 is the most abundantly expressed VGSCs subtype in the adult 
      central nervous system and has been found to contribute to facilitate the 
      hyperexcitability of neurons after electrical induction of status epilepticus 
      (SE). To clarify the exact expression patterns of Nav1.6 during epileptogenesis, 
      we examined the expression of Nav1.6 at protein and mRNA levels in two distinct 
      animal models of temporal lobe epilepsy (TLE) including a post-SE model induced 
      by kainic acid (KA) intrahippocampal injection and a kindling model evoked by 
      pentylenetetrazole (PTZ). A prominent, seizure intensity-dependent increase of 
      Nav1.6 expression in reactive astrocytes was observed in ipsilateral hippocampus 
      of post-SE rats, reaching the peak at 21 days after SE, a time point during the 
      latent stage of epileptogenesis. However, Nav1.6 with low expression level was 
      selectively expressed in the hippocampal neurons rather than astrocytes in 
      PTZ-kindled animals. This seizure-related increase of a VGSCs subtype in reactive 
      astrocytes after SE may represent a new mechanism for signal communication 
      between neuron and glia in the course of epileptogenesis, facilitating the 
      neuronal hyperexcitability.
FAU - Zhu, Hongyan
AU  - Zhu H
AD  - Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, 
      Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
FAU - Zhao, Yuxiao
AU  - Zhao Y
AD  - Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, 
      Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
FAU - Wu, Hao
AU  - Wu H
AD  - Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, 
      Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
FAU - Jiang, Nan
AU  - Jiang N
AD  - Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, 
      Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
FAU - Wang, Ziyi
AU  - Wang Z
AD  - Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, 
      Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
FAU - Lin, Weide
AU  - Lin W
AD  - Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, 
      Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
FAU - Jin, Jiahui
AU  - Jin J
AD  - Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, 
      Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
FAU - Ji, Yonghua
AU  - Ji Y
AD  - Laboratory of Neuropharmacology and Neurotoxicology, School of Life Science, 
      Shanghai University, Nanchen Road 333, Shanghai, 200444, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161201
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Convulsants)
RN  - 0 (NAV1.6 Voltage-Gated Sodium Channel)
RN  - 0 (RNA, Messenger)
RN  - 0 (Scn8a protein, rat)
RN  - SIV03811UC (Kainic Acid)
RN  - WM5Z385K7T (Pentylenetetrazole)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Convulsants/toxicity
MH  - Disease Models, Animal
MH  - Epilepsy, Temporal Lobe/etiology/*genetics/*metabolism
MH  - Hippocampus/metabolism
MH  - Humans
MH  - Kainic Acid/toxicity
MH  - Kindling, Neurologic/genetics/metabolism
MH  - Male
MH  - NAV1.6 Voltage-Gated Sodium Channel/*genetics/*metabolism
MH  - Neurons/metabolism
MH  - Pentylenetetrazole/toxicity
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seizures/genetics/metabolism
MH  - Status Epilepticus/chemically induced/complications
MH  - Time Factors
PMC - PMC5131488
EDAT- 2016/12/03 06:00
MHDA- 2018/06/05 06:00
PMCR- 2016/12/01
CRDT- 2016/12/02 06:00
PHST- 2016/04/14 00:00 [received]
PHST- 2016/11/04 00:00 [accepted]
PHST- 2016/12/02 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2018/06/05 06:00 [medline]
PHST- 2016/12/01 00:00 [pmc-release]
AID - srep38108 [pii]
AID - 10.1038/srep38108 [doi]
PST - epublish
SO  - Sci Rep. 2016 Dec 1;6:38108. doi: 10.1038/srep38108.