PMID- 27906439
OWN - NLM
STAT- MEDLINE
DCOM- 20170802
LR  - 20181202
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 20
IP  - 22
DP  - 2016 Nov
TI  - In vitro neural differentiation of bone marrow stromal cells induced by 
      hepatocyte growth factor and glial cell derived neurotrophic factor.
PG  - 4654-4663
LID - 11765 [pii]
AB  - OBJECTIVE: Bone marrow stromal cells (BMSCs) have great potential for cell-based 
      transplantation therapy in treating neurological disease. However, the best 
      combination of various trophic factors to produce full neural differentiation of 
      BMSCs was still unclear. In our study, we aimed to investigate the neural 
      differentiation capacity of rat BMSCs induced by growth factors including 
      hepatocyte growth factor (HGF) and glial cell-derived neurotrophic factor (GDNF). 
      MATERIALS AND METHODS: Cell counting kit-8 (CCK-8) assay, BrdU cell proliferation 
      assay and flow cytometry were implemented to evaluate whether GDNF and HGF had 
      positive effects on the proliferation of BMSCs. Moreover, the expression of 
      neural specific markers in BMSCs was identified using immunofluorescence and 
      quantitative real-time polymerase chain reaction (RT-PCR) at various time points 
      (1, 7, 14 and 21-day post-induction). RESULTS: CCK-8 and BrdU proliferation 
      analyses demonstrated that only HGF treatment had positive effects on the 
      proliferation of BMSCs on the day 14 and 21 after incubation. RT-PCR and 
      immunofluorescence analyses showed that GDNF and HGF elevated the expression of 
      nestin and NCAM, and the combined application of GDNF and HGF has the most 
      significant effect on day 7 after induction. However, at the day of 14 and 21 
      post-induction, the expression level of nestin and NCAM in GDNF-treatment group 
      was significantly higher than the other three groups. CONCLUSIONS: HGF, not GDNF 
      plays a positive role in BMSCs proliferation, whereas GDNF and HGF are capable of 
      promoting BMSCs to differentiate into neuron-like cells.
FAU - Ma, Q
AU  - Ma Q
AD  - School of Life Science and Biotechnology, Dalian University of Technology, 
      Liaoning Province, Dalian, China. yangqingdalian@163.com.
FAU - Cai, M
AU  - Cai M
FAU - Shang, J-W
AU  - Shang JW
FAU - Yang, J
AU  - Yang J
FAU - Gu, X-Y
AU  - Gu XY
FAU - Liu, W-B
AU  - Liu WB
FAU - Yang, Q
AU  - Yang Q
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Neuroglia/metabolism
MH  - Rats
MH  - Stromal Cells/metabolism
EDAT- 2016/12/03 06:00
MHDA- 2017/08/03 06:00
CRDT- 2016/12/02 06:00
PHST- 2016/12/02 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2017/08/03 06:00 [medline]
AID - 11765 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2016 Nov;20(22):4654-4663.