PMID- 27908496
OWN - NLM
STAT- MEDLINE
DCOM- 20170403
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1477
DP  - 2016 Dec 16
TI  - Mixed-mode acrylamide-based continuous beds bearing tert-butyl groups for 
      capillary electrochromatography synthesized via complexation of 
      N-tert-butylacrylamide with a water-soluble cyclodextrin. Part I: Retention 
      properties.
PG  - 114-126
LID - S0021-9673(16)31548-5 [pii]
LID - 10.1016/j.chroma.2016.11.036 [doi]
AB  - With the aim to improve the understanding of morphology and efficiency 
      properties, we investigate in this series the impact of the complex formation 
      constant of the hydrophobic monomer with respect to statistically 
      methylated-beta-cyclodextrin (Me-beta-CD) on the electrochromatographic properties of 
      highly crosslinked amphiphilic mixed-mode acrylamide-based monolithic stationary 
      phases. Based on our previous work on amphiphilic mixed-mode monolithic 
      stationary phases for capillary electrochromatography (CEC) using 
      N-(1-adamantyl)acrylamide (Ad-AAm) as hydrophobic monomer that forms an extremely 
      strong water-soluble inclusion complex with Me-beta-CD, we now selected 
      N-tert-butylacrylamide (NTBA) as hydrophobic monomer forming an inclusion complex 
      with Me-beta-CD with a much lower value of the formation constant. Mixed-mode 
      monolithic stationary phases are synthesized by in-situ free radical 
      copolymerization of cyclodextrin-solubilized N-tert-butylacrylamide, a water 
      soluble crosslinker (piperazinediacrylamide), a hydrophilic neutral monomer 
      (methacrylamide), and a negatively charged monomer (vinylsulfonic acid) in 
      aqueous medium in bind silane pre-treated fused silica capillaries. The 
      synthesized monolithic stationary phases have both hydrophobic and hydrophilic 
      moieties and can be employed in the reversed-phase mode, in the normal-phase 
      mode, in a mixed-mode or in the hydrophilic interaction liquid chromatography 
      (HILIC) mode (depending on the composition of the mobile phase and on the 
      properties of the solute). Morphology and retention properties of this new type 
      of stationary phase are compared to those reported in our previous series. With a 
      homologues series of alkylphenones it is confirmed that the hydrophobicity 
      (methylene selectivity alpha(meth)) of the stationary phase is strongly dependent on 
      the type of hydrophobic monomer employed. The studies reveal a significant 
      influence of the formation constant of the involved host-guest inclusion complex 
      on the morphology (i.e., the domain size) of the produced monoliths.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Al-Massaedh, Ayat Allah
AU  - Al-Massaedh AA
AD  - Al al-Bayt University, Faculty of Science, Department of Chemistry, 25113 Mafraq, 
      Jordan.
FAU - Pyell, Ute
AU  - Pyell U
AD  - University of Marburg, Department of Chemistry, Hans-Meerwein-Strasse, D-35032 
      Marburg, Germany. Electronic address: pyellu@staff.uni-marburg.de.
LA  - eng
PT  - Journal Article
DEP - 20161121
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Acrylamides)
RN  - 0 (Phenols)
RN  - 0 (beta-Cyclodextrins)
RN  - 059QF0KO0R (Water)
RN  - 20R035KLCI (Acrylamide)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - JV039JZZ3A (betadex)
RN  - XJ13FSH48K (tert-butylacrylamide)
SB  - IM
MH  - Acrylamide/*chemistry
MH  - Acrylamides/*chemistry
MH  - Capillary Electrochromatography/*instrumentation/*methods
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Magnetic Resonance Spectroscopy
MH  - Phenols/analysis
MH  - Polymerization
MH  - Silicon Dioxide/chemistry
MH  - Solubility
MH  - Water/*chemistry
MH  - beta-Cyclodextrins/*chemistry
OTO - NOTNLM
OT  - Acrylamide-based monolithic stationary phase
OT  - Capillary electrochromatography
OT  - Cyclodextrin
OT  - Hydrophilic interaction liquid chromatography
OT  - Mixed-mode retention
OT  - Retention mechanism
EDAT- 2016/12/03 06:00
MHDA- 2017/04/04 06:00
CRDT- 2016/12/03 06:00
PHST- 2016/07/26 00:00 [received]
PHST- 2016/11/08 00:00 [revised]
PHST- 2016/11/20 00:00 [accepted]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2017/04/04 06:00 [medline]
PHST- 2016/12/03 06:00 [entrez]
AID - S0021-9673(16)31548-5 [pii]
AID - 10.1016/j.chroma.2016.11.036 [doi]
PST - ppublish
SO  - J Chromatogr A. 2016 Dec 16;1477:114-126. doi: 10.1016/j.chroma.2016.11.036. Epub 
      2016 Nov 21.