PMID- 27908936
OWN - NLM
STAT- MEDLINE
DCOM- 20171109
LR  - 20181207
IS  - 1943-0264 (Electronic)
IS  - 1943-0264 (Linking)
VI  - 8
IP  - 12
DP  - 2016 Dec 1
TI  - Genome-Editing Technologies: Principles and Applications.
LID - 10.1101/cshperspect.a023754 [doi]
LID - a023754
AB  - Targeted nucleases have provided researchers with the ability to manipulate 
      virtually any genomic sequence, enabling the facile creation of isogenic cell 
      lines and animal models for the study of human disease, and promoting exciting 
      new possibilities for human gene therapy. Here we review three foundational 
      technologies-clustered regularly interspaced short palindromic repeats 
      (CRISPR)-CRISPR-associated protein 9 (Cas9), transcription activator-like 
      effector nucleases (TALENs), and zinc-finger nucleases (ZFNs). We discuss the 
      engineering advances that facilitated their development and highlight several 
      achievements in genome engineering that were made possible by these tools. We 
      also consider artificial transcription factors, illustrating how this technology 
      can complement targeted nucleases for synthetic biology and gene therapy.
CI  - Copyright (c) 2016 Cold Spring Harbor Laboratory Press; all rights reserved.
FAU - Gaj, Thomas
AU  - Gaj T
AD  - Department of Bioengineering, University of California, Berkeley, California 
      94720.
FAU - Sirk, Shannon J
AU  - Sirk SJ
AD  - Department of Chemical Engineering, Stanford University, Stanford, California 
      94305.
FAU - Shui, Sai-Lan
AU  - Shui SL
AD  - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 
      Shanghai, China.
FAU - Liu, Jia
AU  - Liu J
AD  - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 
      Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20161201
PL  - United States
TA  - Cold Spring Harb Perspect Biol
JT  - Cold Spring Harbor perspectives in biology
JID - 101513680
RN  - EC 3.1.- (Endonucleases)
SB  - IM
MH  - Animals
MH  - Clustered Regularly Interspaced Short Palindromic Repeats
MH  - Endonucleases/metabolism
MH  - *Gene Editing
MH  - Humans
PMC - PMC5131771
EDAT- 2016/12/03 06:00
MHDA- 2017/11/10 06:00
PMCR- 2018/12/01
CRDT- 2016/12/03 06:00
PHST- 2016/12/03 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2017/11/10 06:00 [medline]
PHST- 2018/12/01 00:00 [pmc-release]
AID - cshperspect.a023754 [pii]
AID - a023754 [pii]
AID - 10.1101/cshperspect.a023754 [doi]
PST - epublish
SO  - Cold Spring Harb Perspect Biol. 2016 Dec 1;8(12):a023754. doi: 
      10.1101/cshperspect.a023754.