PMID- 27910009
OWN - NLM
STAT- MEDLINE
DCOM- 20180112
LR  - 20180423
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1541
DP  - 2017
TI  - Cancer Cytogenetics: An Introduction.
PG  - 1-10
AB  - The Philadelphia chromosome was the first chromosomal abnormality discovered in 
      cancer using the cytogenetics technique in 1960, and was consistently associated 
      with chronic myeloid leukemia. Over the past five decades, innovative technical 
      advances in the field of cancer cytogenetics have greatly enhanced the detection 
      ability of chromosomal alterations, and have facilitated the research and 
      diagnostic potential of chromosomal studies in neoplasms. These developments 
      notwithstanding, chromosome analysis of a single cell is still the easiest way to 
      delineate and understand the relationship between clonal evolution and disease 
      progression of cancer cells. The use of advanced fluorescence in situ 
      hybridization (FISH) techniques allows for the further identification of 
      chromosomal alterations that are unresolved by the karyotyping method. It 
      overcame many of the drawbacks of assessing the genetic alterations in cancer 
      cells by karyotyping. Subsequently, the development of DNA microarray 
      technologies provides a high-resolution view of the whole genome, which may add 
      massive amounts of new information and opens the field of cancer cytogenomics. 
      Strikingly, cancer cytogenetics does not only provide key information to improve 
      the care of patients with malignancies, but also acts as a guide to identify the 
      genes responsible for the development of these neoplastic states and has led to 
      the emergence of molecularly targeted therapies in the field of personalized 
      medicine.
FAU - Wan, Thomas S K
AU  - Wan TS
AD  - Haematology Division, Department of Anatomical & Cellular Pathology, The Chinese 
      University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong. 
      thomaswan@cuhk.edu.hk.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Comparative Genomic Hybridization
MH  - Computational Biology/methods
MH  - *Cytogenetics/methods
MH  - Databases, Genetic
MH  - Genomics/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Neoplasms/diagnosis/*genetics
OTO - NOTNLM
OT  - Cancer cytogenetics
OT  - FISH
OT  - Karyotyping
OT  - Molecular cytogenetics
OT  - Review
EDAT- 2016/12/03 06:00
MHDA- 2018/01/13 06:00
CRDT- 2016/12/03 06:00
PHST- 2016/12/03 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2018/01/13 06:00 [medline]
AID - 10.1007/978-1-4939-6703-2_1 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1541:1-10. doi: 10.1007/978-1-4939-6703-2_1.