PMID- 27910020
OWN - NLM
STAT- MEDLINE
DCOM- 20180112
LR  - 20180423
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1541
DP  - 2017
TI  - Cytoplasmic Immunoglobulin Light Chain Revelation and Interphase Fluorescence In 
      Situ Hybridization in Myeloma.
PG  - 127-142
AB  - The cytogenetic analysis of plasma cell myeloma (PCM) allows stratification of 
      patients so that prognosis may be determined and appropriate therapeutic options 
      can be discussed. Owing to the patchy nature of the disease in the bone marrow 
      (BM), the low proliferative activity of plasma cells and the cryptic nature of 
      some PCM-associated cytogenetic changes, karyotypic analysis in this disease 
      should be augmented with targeted interphase fluorescence in situ hybridization 
      (FISH). Immunofluorescent revelation of cytoplasmic immunoglobulin light chains, 
      together with interphase FISH (cIg-FISH), allows the identification of plasma 
      cells within a sample so that they may be scored preferentially. This is 
      particularly useful in situations where there are only a small percentage of 
      plasma cells in a sample. Where an underlying myeloid disease is suspected the 
      cIg-FISH-negative cells can be scored separately. Two methods are provided in 
      this chapter: the technique for cIg-FISH in fresh PCM BM samples and a procedure 
      for use in fixed cytogenetics preparations.
FAU - Moore, Sarah
AU  - Moore S
AD  - Genetics and Molecular Pathology, SA Pathology, Frome Road, Adelaide, South 
      Australia, 5000, Australia. sarah.moore2@sa.gov.au.
FAU - Suttle, Jeffrey M
AU  - Suttle JM
AD  - Genetics and Molecular Pathology, SA Pathology, Frome Road, Adelaide, South 
      Australia, 5000, Australia.
FAU - Nicola, Mario
AU  - Nicola M
AD  - Genetics and Molecular Pathology, SA Pathology, Frome Road, Adelaide, South 
      Australia, 5000, Australia.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Immunoglobulin Light Chains)
SB  - IM
MH  - Biomarkers, Tumor
MH  - Bone Marrow/pathology
MH  - Bone Marrow Cells/metabolism/pathology
MH  - Cytoplasm
MH  - *Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoglobulin Light Chains/*metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/*genetics
MH  - Multiple Myeloma/diagnosis/*genetics/*metabolism
MH  - Prognosis
OTO - NOTNLM
OT  - Cytoplasmic light chains
OT  - Fluorescence in situ hybridization
OT  - Plasma cell
OT  - Plasma cell myeloma
OT  - Prognosis
OT  - cIg-FISH
EDAT- 2016/12/03 06:00
MHDA- 2018/01/13 06:00
CRDT- 2016/12/03 06:00
PHST- 2016/12/03 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2018/01/13 06:00 [medline]
AID - 10.1007/978-1-4939-6703-2_12 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1541:127-142. doi: 10.1007/978-1-4939-6703-2_12.