PMID- 27910021
OWN - NLM
STAT- MEDLINE
DCOM- 20180112
LR  - 20220316
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1541
DP  - 2017
TI  - Quantitative Fluorescence In Situ Hybridization (QFISH).
PG  - 143-149
AB  - Fluorescence in situ hybridization (FISH) has a wide spectrum of applications in 
      current molecular cytogenetic and cancer research. This is a unique technique 
      that can be used for chromosomal DNA analysis in all cell types, at all stages of 
      the cell cycle, and at molecular resolution. Recent developments in microscopy 
      and imaging systems have allowed quantification of digital FISH images 
      (quantitative FISH or QFISH) and have provided a new way for molecular 
      cytogenetic analysis at single-cell level. QFISH can be applied for studying 
      chromosome imbalances in interphase nuclei or metaphase spreads, measuring 
      relative DNA content at chromosomal loci and identifying parental origin of 
      homologous chromosomes. Here, a QFISH protocol suitable for the majority of DNA 
      probes using the popular US National Institute of Health developed ImageJ 
      software is described.
FAU - Iourov, Ivan Y
AU  - Iourov IY
AD  - Mental Health Research Center, Zagorodnoe sh. 2, Moscow, 117152, Russia. 
      ivan.iourov@gmail.com.
AD  - Separated Structural Unit "Clinical Research Institute of Pediatrics" named after 
      Y.E. Veltishev, Russian National Research Medical University named after N.I. 
      Pirogov, Ministry of Health of Russian Federation, Moscow, Russia. 
      ivan.iourov@gmail.com.
AD  - Moscow State University of Psychology and Education, Moscow, Russia. 
      ivan.iourov@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosome Aberrations
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Microscopy, Fluorescence/methods
OTO - NOTNLM
OT  - Chromosome abnormalities
OT  - DNA probes
OT  - Fluorescence in situ hybridization
OT  - Interphase
OT  - Nucleus
OT  - QFISH
OT  - Quantification
EDAT- 2016/12/03 06:00
MHDA- 2018/01/13 06:00
CRDT- 2016/12/03 06:00
PHST- 2016/12/03 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2018/01/13 06:00 [medline]
AID - 10.1007/978-1-4939-6703-2_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1541:143-149. doi: 10.1007/978-1-4939-6703-2_13.