PMID- 27910025
OWN - NLM
STAT- MEDLINE
DCOM- 20180112
LR  - 20180423
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1541
DP  - 2017
TI  - Cytogenetics for Biological Dosimetry.
PG  - 189-208
AB  - Cytogenetics is the gold-standard in biological dosimetry for assessing a 
      received dose of ionizing radiation. More modern techniques have recently 
      emerged, but none are as specific as cytogenetic approaches, particularly the 
      dicentric assay. Here, we will focus on the principal cytogenetic techniques used 
      for biological dosimetry: the dicentric assay in metaphase cells, the micronuclei 
      assay in binucleated cells, and the premature condensed chromosome (PCC) assay in 
      interphase cells. New fluorescence in situ hybridization (FISH) techniques (such 
      as telomere-centromere hybridization) have facilitated the analysis of the 
      dicentric assay and have permitted to assess the dose a long time after 
      irradiation by translocation analysis (such as by Tri-color FISH or 
      Multiplex-FISH). Telomere centromere staining of PCCs will make it possible to 
      perform dose assessment within 24 h of exposure in the near future.
FAU - Ricoul, Michelle
AU  - Ricoul M
AD  - PROCyTOX Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), 
      Fontenay-aux-Roses and Universite Paris-Saclay, 18 route du Panorama, 92265, 
      Fontenay-aux-Roses cedex, France.
FAU - Gnana-Sekaran, Tamizh
AU  - Gnana-Sekaran T
AD  - PROCyTOX Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), 
      Fontenay-aux-Roses and Universite Paris-Saclay, 18 route du Panorama, 92265, 
      Fontenay-aux-Roses cedex, France.
FAU - Piqueret-Stephan, Laure
AU  - Piqueret-Stephan L
AD  - PROCyTOX Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), 
      Fontenay-aux-Roses and Universite Paris-Saclay, 18 route du Panorama, 92265, 
      Fontenay-aux-Roses cedex, France.
FAU - Sabatier, Laure
AU  - Sabatier L
AD  - PROCyTOX Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), 
      Fontenay-aux-Roses and Universite Paris-Saclay, 18 route du Panorama, 92265, 
      Fontenay-aux-Roses cedex, France. laure.sabatier@cea.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Chromosome Aberrations/*radiation effects
MH  - Cytogenetic Analysis/*methods
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - Micronuclei, Chromosome-Defective
MH  - Microscopy
MH  - *Radiation, Ionizing
MH  - Radiometry/*methods
MH  - Translocation, Genetic
OTO - NOTNLM
OT  - Biological dosimetry
OT  - Centromere
OT  - Chromosomal aberrations
OT  - Radiation effects
OT  - Telomere
EDAT- 2016/12/03 06:00
MHDA- 2018/01/13 06:00
CRDT- 2016/12/03 06:00
PHST- 2016/12/03 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2018/01/13 06:00 [medline]
AID - 10.1007/978-1-4939-6703-2_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1541:189-208. doi: 10.1007/978-1-4939-6703-2_17.