PMID- 27910906
OWN - NLM
STAT- MEDLINE
DCOM- 20180529
LR  - 20220409
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Dec 2
TI  - Analysis of the Serum Lipid Profile in Polypoidal Choroidal Vasculopathy.
PG  - 38342
LID - 10.1038/srep38342 [doi]
LID - 38342
AB  - Polypoidal choroidal vasculopathy (PCV), the predominant subtype of neovascular 
      age-related macular degeneration in the Asian population, is associated with 
      genetic polymorphism of lipid metabolism. In this study, we performed the 
      untargeted lipidomics approach of ultra-performance liquid chromatography coupled 
      with mass spectrometry (UPLC-MS) to reveal the potential discriminating lipid 
      profile of PCV patients in serum (21 PCV patients and 19 age-matched controls). 
      Unsupervised principal component, supervised orthogonal partial least squares 
      analysis, correlation analysis, and heatmap analysis were performed with the data 
      obtained by UPLC-MS. Forty-one discriminating metabolites were identified. 
      Receiver operating characteristic curve analysis, pathway analysis and functional 
      analysis were performed subsequently, and platelet-activating factor (PAF) was 
      further selected as the key indicator of the distinct lipid metabolism in PCV 
      patients. Finally, the serum level of PAF was validated by enzyme-linked 
      immunosorbent assay, which is significantly higher in PCV patients compared to 
      controls (65 PCV patients and 63 age-matched controls, p < 0.0001), consistent 
      with the UPLC-MS analysis. Our results suggested that PAF is considered as the 
      major indicator of the distinct lipid metabolism in PCV patients.
FAU - Li, Miaoling
AU  - Li M
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Zhang, Xiongze
AU  - Zhang X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Liao, Nanying
AU  - Liao N
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Ye, Baikang
AU  - Ye B
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Peng, Yuting
AU  - Peng Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Ji, Yuying
AU  - Ji Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Wen, Feng
AU  - Wen F
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161202
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (Platelet Activating Factor)
SB  - IM
MH  - Aged
MH  - Biomarkers/blood
MH  - Case-Control Studies
MH  - Choroid/blood supply/metabolism/pathology
MH  - Choroidal Neovascularization/blood/*diagnosis/pathology
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Least-Squares Analysis
MH  - Lipid Metabolism
MH  - Macular Degeneration/blood/*diagnosis/pathology
MH  - Male
MH  - *Metabolome
MH  - Middle Aged
MH  - Platelet Activating Factor/*genetics/metabolism
MH  - Principal Component Analysis
MH  - ROC Curve
PMC - PMC5133601
EDAT- 2016/12/03 06:00
MHDA- 2018/05/31 06:00
PMCR- 2016/12/02
CRDT- 2016/12/03 06:00
PHST- 2016/01/06 00:00 [received]
PHST- 2016/11/09 00:00 [accepted]
PHST- 2016/12/03 06:00 [entrez]
PHST- 2016/12/03 06:00 [pubmed]
PHST- 2018/05/31 06:00 [medline]
PHST- 2016/12/02 00:00 [pmc-release]
AID - srep38342 [pii]
AID - 10.1038/srep38342 [doi]
PST - epublish
SO  - Sci Rep. 2016 Dec 2;6:38342. doi: 10.1038/srep38342.