PMID- 27913826
OWN - NLM
STAT- MEDLINE
DCOM- 20170314
LR  - 20181202
IS  - 1432-0800 (Electronic)
IS  - 0007-4861 (Linking)
VI  - 98
IP  - 1
DP  - 2017 Jan
TI  - Partial Gene Sequencing of CYP1A, Vitellogenin, and Metallothionein in 
      Mosquitofish Gambusia yucatana and Gambusia sexradiata.
PG  - 41-45
LID - 10.1007/s00128-016-1994-1 [doi]
AB  - Ground characteristics in the Yucatan Peninsula make recovery and treatment of 
      wastewater very expensive. This situation has contributed to an increase of 
      pollutants in the aquifer. Unfortunately, studies related to the effects of those 
      pollutants in native organisms are scarce. The aim of this work was to obtain 
      partial sequences of widely known genes used as biomarkers of pollutant effect in 
      Gambusia yucatana and Gambusia sexradiata. The studied genes were: cytochrome 
      P450 1A (CYP1A); vitellogenin (VTG); metallothionein (MT), and two housekeeping 
      genes, 18S and beta-actin. From reported sequences of Gambusia affinis, primers were 
      designed and amplification was done in the local Gambusia species exposed for 
      48 h to gasoline (100 microL/L, stirred for 24 h pre-exposure). Preliminary results 
      revealed partial sequences of all genes with an approximate average length of 
      200 bp. BLAST analysis of found sequences indicated a minimum of 97% identity 
      with reported sequences for G. affinis or Gambusia holbrooki showing great 
      similarity.
FAU - Vazquez-Euan, Roberto
AU  - Vazquez-Euan R
AD  - Unidad de Quimica en Sisal, Facultad de Quimica, Universidad Nacional Autonoma de 
      Mexico, Puerto de Abrigo s/n, 97356, Sisal, YUC, Mexico.
AD  - CONACYT-Departamento de Investigaciones Cientificas y Tecnologicas de la 
      Universidad de Sonora, Hermosillo, SON, Mexico.
FAU - Escalante-Herrera, Karla S
AU  - Escalante-Herrera KS
AD  - Unidad Multidisciplinaria de Docencia e Investigacion, Facultad de Ciencias, 
      Universidad Nacional Autonoma de Mexico, Puerto de Abrigo s/n, 97356, Sisal, YUC, 
      Mexico.
FAU - Rodriguez-Fuentes, Gabriela
AU  - Rodriguez-Fuentes G
AD  - Unidad de Quimica en Sisal, Facultad de Quimica, Universidad Nacional Autonoma de 
      Mexico, Puerto de Abrigo s/n, 97356, Sisal, YUC, Mexico. grf@unam.mx.
LA  - eng
PT  - Journal Article
DEP - 20161202
PL  - United States
TA  - Bull Environ Contam Toxicol
JT  - Bulletin of environmental contamination and toxicology
JID - 0046021
RN  - 0 (Actins)
RN  - 0 (Gasoline)
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (Vitellogenins)
RN  - 0 (Water Pollutants, Chemical)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - 9038-94-2 (Metallothionein)
SB  - IM
MH  - Actins/genetics
MH  - Animals
MH  - Base Sequence
MH  - Cyprinodontiformes/*genetics
MH  - Cytochrome P-450 Enzyme System/*genetics
MH  - Female
MH  - Gasoline/toxicity
MH  - Genetic Markers
MH  - Metallothionein/*genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - Sequence Analysis, DNA
MH  - Vitellogenins/*genetics
MH  - Water Pollutants, Chemical/toxicity
OTO - NOTNLM
OT  - CYP1A
OT  - Gambusia
OT  - Metallothioneins
OT  - Mosquitofish
OT  - Vitellogenin
EDAT- 2016/12/04 06:00
MHDA- 2017/03/16 06:00
CRDT- 2016/12/04 06:00
PHST- 2016/05/18 00:00 [received]
PHST- 2016/11/29 00:00 [accepted]
PHST- 2016/12/04 06:00 [pubmed]
PHST- 2017/03/16 06:00 [medline]
PHST- 2016/12/04 06:00 [entrez]
AID - 10.1007/s00128-016-1994-1 [pii]
AID - 10.1007/s00128-016-1994-1 [doi]
PST - ppublish
SO  - Bull Environ Contam Toxicol. 2017 Jan;98(1):41-45. doi: 
      10.1007/s00128-016-1994-1. Epub 2016 Dec 2.