PMID- 27913867
OWN - NLM
STAT- MEDLINE
DCOM- 20170320
LR  - 20220317
IS  - 1432-1335 (Electronic)
IS  - 0171-5216 (Linking)
VI  - 143
IP  - 4
DP  - 2017 Apr
TI  - Comparison of different concepts for interpretation of chromosomal aberrations in 
      urothelial cells detected by fluorescence in situ hybridization.
PG  - 677-685
LID - 10.1007/s00432-016-2310-5 [doi]
AB  - PURPOSE: Urine fluorescence in situ hybridization (FISH) has become a broadly 
      used marker for noninvasive detection of bladder cancer (BC). However, it has 
      been discussed whether the interpretation algorithm proposed by the manufacturer 
      could be improved. Aim of the present study was to compare alternative evaluation 
      strategies of FISH for detection of BC. METHODS: We included 1048 patients 
      suspicious for BC, who underwent urine FISH examination before cystoscopy 
      (diagnostic cohort). Herefrom, we selected 122 patients (prognostic cohort) with 
      a history of non-muscle-invasive BC who were cystoscopically tumor free and 
      received FISH analysis ahead of a follow-up period of 24 months. FISH results 
      were interpreted by the algorithms of UroVysion, Bubendorf et al. and Zellweger 
      et al. RESULTS: In the diagnostic cohort, 228 patients (21.8%) had BC at time of 
      evaluation; in the prognostic cohort 39 patients (32.0%) experienced tumor 
      recurrence. Alterations in chromosome 3, 7 and 17 correlated with the presence of 
      BC. Relative loss of 9p21 was associated with BC and higher risk for progression. 
      The evaluation strategy proposed by Zellweger et al. showed highest accuracy of 
      all FISH assessments. Performance of evaluation strategies differed in voided 
      urine samples and samples obtained after mechanical manipulation. CONCLUSIONS: 
      The performance of FISH in BC diagnosis strongly depends on the interpretation 
      criteria. Alternative evaluation methods partly show superior diagnostic 
      performance compared to the manufacturer's algorithm. The introduction of 
      specific cutoffs for tetraploid cells improves specificity. Further modifications 
      of the interpretation algorithm of the Urovysion((R)) FISH assay have the potential 
      to positively affect the value of this test in diagnosis and surveillance of BC.
FAU - Mischinger, Johannes
AU  - Mischinger J
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Guttenberg, Lutz Philipp
AU  - Guttenberg LP
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Hennenlotter, Jorg
AU  - Hennenlotter J
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Gakis, Georgios
AU  - Gakis G
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Aufderklamm, Stefan
AU  - Aufderklamm S
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Rausch, Steffen
AU  - Rausch S
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Neumann, Eva
AU  - Neumann E
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Bedke, Jens
AU  - Bedke J
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Kruck, Stefan
AU  - Kruck S
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Schwentner, Christian
AU  - Schwentner C
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Stenzl, Arnulf
AU  - Stenzl A
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany.
FAU - Todenhofer, Tilman
AU  - Todenhofer T
AD  - Department of Urology, University Hospital Tuebingen, Eberhard-Karls University, 
      Hoppe-Seyler Street 3, 72076, Tubingen, Germany. 
      tilman.todenhoefer@med.uni-tuebingen.de.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20161202
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Urothelium/*cytology
MH  - Young Adult
OTO - NOTNLM
OT  - Bladder cancer
OT  - Diagnostic and prognostic value
OT  - FISH
OT  - Urine marker
OT  - Urovysion
EDAT- 2016/12/04 06:00
MHDA- 2017/03/21 06:00
CRDT- 2016/12/04 06:00
PHST- 2016/09/21 00:00 [received]
PHST- 2016/11/01 00:00 [accepted]
PHST- 2016/12/04 06:00 [pubmed]
PHST- 2017/03/21 06:00 [medline]
PHST- 2016/12/04 06:00 [entrez]
AID - 10.1007/s00432-016-2310-5 [pii]
AID - 10.1007/s00432-016-2310-5 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 2017 Apr;143(4):677-685. doi: 10.1007/s00432-016-2310-5. 
      Epub 2016 Dec 2.