PMID- 27918990
OWN - NLM
STAT- MEDLINE
DCOM- 20170418
LR  - 20231112
IS  - 1095-7103 (Electronic)
IS  - 0021-9797 (Print)
IS  - 0021-9797 (Linking)
VI  - 490
DP  - 2017 Mar 15
TI  - Effects of tripolyphosphate on cellular uptake and RNA interference efficiency of 
      chitosan-based nanoparticles in Raw 264.7 macrophages.
PG  - 520-528
LID - S0021-9797(16)30973-0 [pii]
LID - 10.1016/j.jcis.2016.11.088 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a major pro-inflammatory cytokine that is 
      mainly secreted by macrophages during inflammation. Here, we synthesized a series 
      of N-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chlorides (HTCCs), and then 
      used a complex coacervation technique or tripolyphosphate (TPP)-assisted 
      ionotropic gelation strategy to complex the HTCCs with TNF-alpha siRNA (siTNF) to 
      form nanoparticles (NPs). The resultant NPs had a desirable particle size 
      (210-279nm), a slightly positive zeta potential (14-22mV), and negligible 
      cytotoxicity against Raw 264.7 macrophages and colon-26 cells. Subsequent 
      cellular uptake tests demonstrated that the introduction of TPP to the NPs 
      markedly increased their cellular uptake efficiency (to nearly 100%) compared 
      with TPP-free NPs, and yielded a correspondingly higher intracellular 
      concentration of siRNA. Critically, in vitro gene silencing experiments revealed 
      that all of the TPP-containing NPs showed excellent efficiency in inhibiting the 
      mRNA expression level of TNF-alpha (by approximately 85-92%, which was much higher 
      than that obtained using Oligofectamine/siTNF complexes). Collectively, these 
      results obviously suggest that our non-toxic TPP-containing chitosan-based NPs 
      can be exploited as efficient siTNF carriers for the treatment of inflammatory 
      diseases.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Xiao, Bo
AU  - Xiao B
AD  - Institute for Clean Energy and Advanced Materials, Faculty of Materials and 
      Energy, Southwest University, Chongqing 400715, PR China; Institute for 
      Biomedical Science, Center for Diagnostics and Therapeutics, Georgia State 
      University, Atlanta 30302, USA. Electronic address: bxiao@gsu.edu.
FAU - Ma, Panpan
AU  - Ma P
AD  - Institute for Clean Energy and Advanced Materials, Faculty of Materials and 
      Energy, Southwest University, Chongqing 400715, PR China.
FAU - Ma, Lijun
AU  - Ma L
AD  - Institute for Clean Energy and Advanced Materials, Faculty of Materials and 
      Energy, Southwest University, Chongqing 400715, PR China.
FAU - Chen, Qiubing
AU  - Chen Q
AD  - Institute for Clean Energy and Advanced Materials, Faculty of Materials and 
      Energy, Southwest University, Chongqing 400715, PR China.
FAU - Si, Xiaoying
AU  - Si X
AD  - Institute for Clean Energy and Advanced Materials, Faculty of Materials and 
      Energy, Southwest University, Chongqing 400715, PR China.
FAU - Walter, Lewins
AU  - Walter L
AD  - Institute for Biomedical Science, Center for Diagnostics and Therapeutics, 
      Georgia State University, Atlanta 30302, USA.
FAU - Merlin, Didier
AU  - Merlin D
AD  - Institute for Biomedical Science, Center for Diagnostics and Therapeutics, 
      Georgia State University, Atlanta 30302, USA; Atlanta Veterans Affairs Medical 
      Center, Decatur 30033, USA.
LA  - eng
GR  - I01 BX002526/BX/BLRD VA/United States
GR  - R01 DK071594/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20161126
PL  - United States
TA  - J Colloid Interface Sci
JT  - Journal of colloid and interface science
JID - 0043125
RN  - 0 (N-(2-hydroxypropyl)-3-trimethylammonium chitosan)
RN  - 0 (Polyphosphates)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9012-76-4 (Chitosan)
RN  - NU43IAG5BC (triphosphoric acid)
SB  - IM
MH  - Animals
MH  - Chitosan/*analogs & derivatives/chemistry
MH  - Macrophages/cytology/*metabolism
MH  - Mice
MH  - Nanoparticles/*chemistry
MH  - Polyphosphates/*chemistry
MH  - Quaternary Ammonium Compounds/*chemistry
MH  - RAW 264.7 Cells
MH  - *RNA Interference
MH  - RNA, Small Interfering/*administration & dosage/genetics/pharmacokinetics
MH  - Tumor Necrosis Factor-alpha/*genetics
PMC - PMC5222762
MID - NIHMS834085
OTO - NOTNLM
OT  - Chitosan
OT  - Macrophage
OT  - N-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chloride
OT  - Nanoparticle
OT  - RNA interference
OT  - Tripolyphosphate
EDAT- 2016/12/06 06:00
MHDA- 2017/04/19 06:00
PMCR- 2018/03/15
CRDT- 2016/12/06 06:00
PHST- 2016/09/19 00:00 [received]
PHST- 2016/11/24 00:00 [revised]
PHST- 2016/11/25 00:00 [accepted]
PHST- 2016/12/06 06:00 [pubmed]
PHST- 2017/04/19 06:00 [medline]
PHST- 2016/12/06 06:00 [entrez]
PHST- 2018/03/15 00:00 [pmc-release]
AID - S0021-9797(16)30973-0 [pii]
AID - 10.1016/j.jcis.2016.11.088 [doi]
PST - ppublish
SO  - J Colloid Interface Sci. 2017 Mar 15;490:520-528. doi: 
      10.1016/j.jcis.2016.11.088. Epub 2016 Nov 26.