PMID- 27924719
OWN - NLM
STAT- MEDLINE
DCOM- 20170526
LR  - 20170526
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 79
IP  - 22-23
DP  - 2016
TI  - Quantification of obeche wood allergen: Development of a sensitive sandwich-ELISA 
      for occupational exposure assessment.
PG  - 1070-1077
AB  - Obeche wood is a prominent cause of allergic occupational asthma. To reduce the 
      risk of immunoglobulin E (IgE)-mediated sensitization it is important to assess 
      airborne obeche wood allergen concentrations at exposed workplaces. Therefore, a 
      highly sensitive obeche wood allergen immunoassay was developed and applicability 
      was proven on airborne passive dust samples in Spanish wood workshops. Obeche 
      wood sandwich enzyme-linked immunosorbent assay (ELISA) polyclonal antibodies 
      (pAbs) were developed. Test specificity was verified by different wood and mold 
      extracts. Obeche wood allergen monitoring was conducted in four Spanish wood 
      workshops, including wood-dust-exposed and nonexposed areas inside and outside 
      the workplaces, as well as controls. Dust was collected with electrostatic dust 
      collectors (EDC). Measuring range of the obeche wood sandwich-ELISA was between 
      36 pg/ml and 1.6 microg/ml. The test system showed only marginal reactivity to other 
      hardwoods and no reactivity to softwoods and molds. Obeche allergen was detected 
      in all EDC from workplaces. The highest concentration was measured in the 
      workshop with the longest obeche wood exposure (geometric mean [GM]: 7548 
      microg/m(2)); shorter obeche wood processing periods resulted in lower amounts of 
      allergen (GM: 29 microg/m(2)). Obeche wood allergen transfer from exposed workplaces 
      to nonexposed areas inside and outside the workshop was assessed. In control EDC 
      from nonexposed facilities/homes no obeche wood allergen was found. The new 
      obeche wood sandwich-ELISA is a valid tool to quantify obeche allergen exposure. 
      Evidence indicates it will be possible to monitor obeche allergen exposure during 
      different processes, as well as transfer effects in nonexposed areas.
FAU - Kespohl, S
AU  - Kespohl S
AD  - a Institute for Prevention and Occupational Medicine of the German Social 
      Accident Insurance , Institute of the Ruhr-Universitat Bochum (IPA) , Bochum , 
      Germany.
FAU - Campo, P
AU  - Campo P
AD  - b UGC Allergy, Regional Hospital of Malaga-IBIMA , Malaga , Espania.
FAU - Zahradnik, E
AU  - Zahradnik E
AD  - a Institute for Prevention and Occupational Medicine of the German Social 
      Accident Insurance , Institute of the Ruhr-Universitat Bochum (IPA) , Bochum , 
      Germany.
FAU - Maryska, S
AU  - Maryska S
AD  - a Institute for Prevention and Occupational Medicine of the German Social 
      Accident Insurance , Institute of the Ruhr-Universitat Bochum (IPA) , Bochum , 
      Germany.
FAU - Aranda-Guerrero, A
AU  - Aranda-Guerrero A
AD  - b UGC Allergy, Regional Hospital of Malaga-IBIMA , Malaga , Espania.
FAU - Rodriguez, J
AU  - Rodriguez J
AD  - b UGC Allergy, Regional Hospital of Malaga-IBIMA , Malaga , Espania.
FAU - Bruning, T
AU  - Bruning T
AD  - a Institute for Prevention and Occupational Medicine of the German Social 
      Accident Insurance , Institute of the Ruhr-Universitat Bochum (IPA) , Bochum , 
      Germany.
FAU - Raulf, M
AU  - Raulf M
AD  - a Institute for Prevention and Occupational Medicine of the German Social 
      Accident Insurance , Institute of the Ruhr-Universitat Bochum (IPA) , Bochum , 
      Germany.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Allergens)
RN  - 0 (Dust)
SB  - IM
MH  - Allergens/*analysis
MH  - Dust/*analysis
MH  - Environmental Monitoring/instrumentation
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Humans
MH  - Malvaceae/*chemistry
MH  - *Occupational Exposure
MH  - Spain
MH  - Wood/*analysis
EDAT- 2016/12/08 06:00
MHDA- 2017/05/27 06:00
CRDT- 2016/12/08 06:00
PHST- 2016/12/08 06:00 [entrez]
PHST- 2016/12/08 06:00 [pubmed]
PHST- 2017/05/27 06:00 [medline]
AID - 10.1080/15287394.2016.1219577 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2016;79(22-23):1070-1077. doi: 
      10.1080/15287394.2016.1219577.