PMID- 27925264
OWN - NLM
STAT- MEDLINE
DCOM- 20170524
LR  - 20170524
IS  - 1365-2052 (Electronic)
IS  - 0268-9146 (Linking)
VI  - 48
IP  - 3
DP  - 2017 Jun
TI  - Three intronic lncRNAs with monoallelic expression derived from the MEG8 gene in 
      cattle.
PG  - 272-277
LID - 10.1111/age.12527 [doi]
AB  - The field of long noncoding RNA (lncRNA) research has been rapidly advancing in 
      recent years. Antisense lncRNAs, intergenetic lncRNAs and enhancer lncRNAs can 
      regulate genomic imprinting, which leads to parent-origin-specific monoalletic 
      expression of genes. However, the function of intronic ncRNAs in genomic 
      imprinting remains unclear. Previously, we obtained the cDNA sequence of cattle 
      MEG8 gene, which is located in the DLK1-DIO3 imprinted clusters of cattle 
      chromosome 21. In this study, we undertook a systematic search for transcripts 
      mapping to the MEG8 intronic region and identified three novel lncRNAs, named 
      MEG8 intronic RNA 1 (MEG8-IT1), MEG8 intronic RNA 2 (MEG8-IT2) and MEG8 intronic 
      RNA 3 (MEG8-IT3) according to the GENCODE annotated bibliography. We 
      characterized the expression pattern of these lncRNAs using RT-PCR in adult 
      cattle tissues, and they were expressed in all tested eight tissues, similar to 
      the expression pattern of MEG8. The allele-specific expression of three novel 
      lncRNAs was assessed using a polymorphism-based sequencing approach. Three single 
      nucleotide polymorphism sites were identified in these three lncRNAs. We found 
      that the three lncRNAs showed monoallelic expression in the analyzed tissues, 
      suggesting that they may be imprinted in cattle. These results expand the number 
      of known monoallelically expressed lncRNAs from the DLK1-DIO3 domain and 
      contribute to further investigation of lncRNA regulatory mechanisms and function.
CI  - (c) 2016 Stichting International Foundation for Animal Genetics.
FAU - Yang, W
AU  - Yang W
AD  - Department of Biochemistry and Molecular Biology, College of Life Science, Hebei 
      Agriculture University, Baoding, 071001, China.
FAU - Li, D
AU  - Li D
AD  - College of Life Science and Life Engineering, Hebei Science and Technology 
      University, Shijiazhuang, 050018, China.
FAU - Wang, G
AU  - Wang G
AD  - Department of Biochemistry and Molecular Biology, College of Life Science, Hebei 
      Agriculture University, Baoding, 071001, China.
FAU - Zhang, C
AU  - Zhang C
AD  - Department of Biochemistry and Molecular Biology, College of Life Science, Hebei 
      Agriculture University, Baoding, 071001, China.
FAU - Zhang, M
AU  - Zhang M
AD  - Department of Biochemistry and Molecular Biology, College of Life Science, Hebei 
      Agriculture University, Baoding, 071001, China.
FAU - Zhang, W
AU  - Zhang W
AD  - Department of Biochemistry and Molecular Biology, College of Life Science, Hebei 
      Agriculture University, Baoding, 071001, China.
FAU - Li, S
AU  - Li S
AD  - Department of Biochemistry and Molecular Biology, College of Life Science, Hebei 
      Agriculture University, Baoding, 071001, China.
LA  - eng
SI  - GENBANK/BTU85042
SI  - GENBANK/DN527838
SI  - GENBANK/DN525095
SI  - GENBANK/DY040751
SI  - GENBANK/CN440057
SI  - GENBANK/CB169136
SI  - GENBANK/AV598609
SI  - GENBANK/DY119069
SI  - GENBANK/EE353306
SI  - GENBANK/AV592951
SI  - GENBANK/BM105278
SI  - GENBANK/CO879356
SI  - GENBANK/KU553268
SI  - GENBANK/KU553269
SI  - GENBANK/KU553270
PT  - Journal Article
DEP - 20161207
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Cattle/*genetics
MH  - Gene Expression
MH  - Genomic Imprinting
MH  - *Introns
MH  - Polymorphism, Single Nucleotide
MH  - RNA, Long Noncoding/*genetics
OTO - NOTNLM
OT  - DLK1-DIO3 imprinted domain
OT  - RT-PCR
OT  - gene expression
OT  - genomic imprinting
EDAT- 2016/12/08 06:00
MHDA- 2017/05/26 06:00
CRDT- 2016/12/08 06:00
PHST- 2016/10/18 00:00 [accepted]
PHST- 2016/12/08 06:00 [pubmed]
PHST- 2017/05/26 06:00 [medline]
PHST- 2016/12/08 06:00 [entrez]
AID - 10.1111/age.12527 [doi]
PST - ppublish
SO  - Anim Genet. 2017 Jun;48(3):272-277. doi: 10.1111/age.12527. Epub 2016 Dec 7.