PMID- 27925288
OWN - NLM
STAT- MEDLINE
DCOM- 20170414
LR  - 20170414
IS  - 1348-0421 (Electronic)
IS  - 0385-5600 (Linking)
VI  - 60
IP  - 12
DP  - 2016 Dec
TI  - Genomic sequence and virulence evaluation of MN184A-like porcine reproductive and 
      respiratory syndrome virus in Japan.
PG  - 824-834
LID - 10.1111/1348-0421.12455 [doi]
AB  - In this study, a porcine reproductive and respiratory syndrome virus (PRRSV) that 
      was isolated from a 9-week-old diseased pig on a farm in Japan with a high 
      mortality rate during 2007-2008 was characterized. This unique isolate, 
      designated as Jpn5-37, did not have a high nucleotide identity in open reading 
      frame 5 against any Japanese isolates. Among all available type 2 PRRSV complete 
      genome sequences, Jpn5-37 shared the highest nucleotide identity (93.6%) with 
      virulent strain MN184A. The genomic characteristics of Jpn5-37 were highly 
      conserved with respect to the virulent MN184A, including a continuous eight amino 
      acid deletion in the nonstructural protein 2 region. Moreover, virus 
      distribution, viremia and the gross and microscopic characteristics of lesions 
      were investigated in pigs 10 days post-inoculation to elucidate the pathogenicity 
      of the isolate. Intranasal inoculation was found to rapidly result in viremia and 
      dissemination of the Jpn5-37 isolate to several tissues in a similar manner to 
      EDRD1; however, the amounts of Jpn5-37 RNA in serum were significantly greater. 
      Similarly, the quantities of Jpn5-37 viral RNA in all organs tested tended to be 
      higher than with EDRD1 infection. Mean rectal temperatures were significantly 
      higher in the Jpn5-37-inoculated than in the control group at 4 and 6 days post 
      infection (dpi) and in the EDRD1-inoculated group at 6 and 8 dpi. These results 
      suggest that the Jpn5-37 strain replicates and is more efficiently distributed to 
      the organs than is EDRD1 under the same conditions.
CI  - (c) 2016 The Societies and John Wiley & Sons Australia, Ltd.
FAU - Iseki, Hiroshi
AU  - Iseki H
AD  - Viral Disease and Epidemiological Research Division.
FAU - Morozumi, Takeya
AU  - Morozumi T
AD  - Animal Research Division, Institute of Japan Association for Techno-innovation in 
      Agriculture, Forestry and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, 
      Ibaraki 305-0854.
FAU - Takagi, Michihiro
AU  - Takagi M
AD  - Viral Disease and Epidemiological Research Division.
FAU - Kawashima, Kenji
AU  - Kawashima K
AD  - Viral Disease and Epidemiological Research Division.
FAU - Shibahara, Tomoyuki
AU  - Shibahara T
AD  - Pathology and Pathophysiology Research Division, National Institute of Animal 
      Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, 
      Tsukuba, Ibaraki, 305-0856.
FAU - Uenishi, Hirohide
AU  - Uenishi H
AD  - Animal Bioregulation Unit, Division of Animal Sciences, Institute of 
      Agrobiological Sciences, National Agriculture and Food Research Organization, 1-2 
      Owashi, Tsukuba, Ibaraki 305-8634.
FAU - Tsunemitsu, Hiroshi
AU  - Tsunemitsu H
AD  - Dairy Hygiene Research Division, National Institute of Animal Health, National 
      Agriculture and Food Research Organization, Hitsujigaoka, Toyohira-ku, Sapporo, 
      Hokkaido, 062-0045, Japan.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animal Structures/virology
MH  - Animals
MH  - Cluster Analysis
MH  - *Genome, Viral
MH  - Japan
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification/*pathogenicity
MH  - RNA, Viral/blood/*genetics
MH  - *Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Time Factors
MH  - Viral Envelope Proteins/genetics
MH  - Viremia
MH  - Virulence
OTO - NOTNLM
OT  - consensus oligonucleotide reverse transcription-multiple displacement 
      amplification
OT  - genome sequence
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2016/12/08 06:00
MHDA- 2017/04/15 06:00
CRDT- 2016/12/08 06:00
PHST- 2016/05/09 00:00 [received]
PHST- 2016/11/01 00:00 [revised]
PHST- 2016/11/21 00:00 [accepted]
PHST- 2016/12/08 06:00 [pubmed]
PHST- 2017/04/15 06:00 [medline]
PHST- 2016/12/08 06:00 [entrez]
AID - 10.1111/1348-0421.12455 [doi]
PST - ppublish
SO  - Microbiol Immunol. 2016 Dec;60(12):824-834. doi: 10.1111/1348-0421.12455.