PMID- 27928553
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240326
IS  - 2452-2198 (Print)
IS  - 2452-2198 (Electronic)
IS  - 2452-2198 (Linking)
VI  - 1
DP  - 2016 Jun
TI  - Proliferation of the biocontrol agent Fusarium oxysporum f. sp. strigae and its 
      impact on indigenous rhizosphere fungal communities in maize under different 
      agro-ecologies.
PG  - 17-25
AB  - Our objectives were to (1) monitor the proliferation of the biocontrol agent 
      (BCA) Fusarium oxysporum f. sp. strigae strain "Foxy-2", an effective soil-borne 
      BCA against the parasitic weed Striga hermonthica, in the rhizosphere of maize 
      under different agro-ecologies, and (2) investigate its impact on indigenous 
      rhizosphere fungal community abundance and composition. Field experiments were 
      conducted in Busia and Homa Bay districts in western Kenya during two cropping 
      seasons to account for effects of soil type, climate, growth stage and 
      seasonality. Maize seeds were coated with or without "Foxy-2" and soils were 
      artificially infested with S. hermonthica seeds. One treatment with nitrogen rich 
      organic residues (Tithonia diversifolia) was established to compensate 
      hypothesized resource competition between "Foxy-2" and the indigenous fungal 
      community. Rhizosphere soil samples collected at three growth stages (i.e., EC30, 
      EC60, EC90) of maize were subjected to abundance measurement of "Foxy-2" and 
      total indigenous fungi using quantitative polymerase chain reaction (qPCR) 
      analysis. Terminal restriction fragment length polymorphism (TRFLP) analysis was 
      used to assess potential alterations in the fungal community composition in 
      response to "Foxy-2" presence. "Foxy-2" proliferated stronger in the soils with a 
      sandy clay texture (Busia) than in those with a loamy sand texture (Homa Bay) and 
      revealed slightly higher abundance in the second season. "Foxy-2" had, however, 
      only a transient suppressive effect on total indigenous fungal abundance which 
      ceased in the second season and was further markedly compensated after addition 
      of T. diversifolia residues. Likewise, community structure of the indigenous 
      fungal community was mainly altered by maize growth stages, but not by "Foxy-2". 
      In conclusion, no adverse effects of "Foxy-2" inoculation on indigenous fungal 
      rhizosphere communities were observed corroborating the safety of this BCA under 
      the given agro-ecologies.
FAU - Zimmermann, Judith
AU  - Zimmermann J
AD  - Institute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), 
      University of Hohenheim, Stuttgart, Germany.
FAU - Musyoki, Mary K
AU  - Musyoki MK
AD  - Institute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), 
      University of Hohenheim, Stuttgart, Germany.
FAU - Cadisch, Georg
AU  - Cadisch G
AD  - Institute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), 
      University of Hohenheim, Stuttgart, Germany.
FAU - Rasche, Frank
AU  - Rasche F
AD  - Institute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), 
      University of Hohenheim, Stuttgart, Germany.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Rhizosphere
JT  - Rhizosphere
JID - 101696005
PMC - PMC5125437
OTO - NOTNLM
OT  - 18S rDNA
OT  - Fusarium oxysporum f.sp. strigae
OT  - Quantitative PCR
OT  - TRFLP analysis
OT  - maize
EDAT- 2016/12/09 06:00
MHDA- 2016/12/09 06:01
PMCR- 2016/06/01
CRDT- 2016/12/09 06:00
PHST- 2016/03/30 00:00 [received]
PHST- 2016/06/13 00:00 [revised]
PHST- 2016/06/16 00:00 [accepted]
PHST- 2016/12/09 06:00 [entrez]
PHST- 2016/12/09 06:00 [pubmed]
PHST- 2016/12/09 06:01 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - S2452-2198(16)30011-8 [pii]
AID - 10.1016/j.rhisph.2016.06.002 [doi]
PST - ppublish
SO  - Rhizosphere. 2016 Jun;1:17-25. doi: 10.1016/j.rhisph.2016.06.002.