PMID- 27929393
OWN - NLM
STAT- MEDLINE
DCOM- 20170403
LR  - 20181113
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 8
IP  - 12
DP  - 2016 Dec 5
TI  - Xylobiose, an Alternative Sweetener, Ameliorates Diabetes-Related Metabolic 
      Changes by Regulating Hepatic Lipogenesis and miR-122a/33a in db/db Mice.
LID - 791
AB  - Type 2 diabetes is a major public health concern worldwide. Xylobiose (XB) 
      consists of two molecules of d-xylose and is a major disaccharide in 
      xylooligosaccharides that are used as prebiotics. We hypothesized that XB could 
      regulate diabetes-related metabolic and genetic changes via microRNA expression 
      in db/db mice. For six weeks, C57BL/KsJ-db/db mice received 5% XB as part of the 
      total sucrose content of their diet. XB supplementation improved glucose 
      tolerance with reduced levels of OGTT AUC, fasting blood glucose, HbA1c, insulin, 
      and HOMA-IR. Furthermore, XB supplementation decreased the levels of total 
      triglycerides, total cholesterol, and LDL-C. The expression levels of miR-122a 
      and miR-33a were higher and lower in the XB group, respectively. In the liver, 
      expressions of the lipogenic genes, including, fatty acid synthase (FAS), 
      peroxisome proliferator activated receptor gamma (PPARgamma), sterol regulatory 
      element-binding protein-1C (SREBP-1C), sterol regulatory element-binding 
      protein-2 (SREBP-2), acetyl-CoA carboxylase (ACC), HMG-CoA reductase (HMGCR), 
      ATP-binding cassette transporter G5/G8 (ABCG5/8), cholesterol 7 alpha-hydroxylase 
      (CYP7A1), and sterol 12-alpha-hydroxylase (CYP8B1), as well as oxidative stress 
      markers, including superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), 
      glutathione peroxidase (GPX), and catalase, were also regulated by XB 
      supplementation. XB supplementation inhibited the mRNA expressions levels of the 
      pro-inflammatory cytokines, tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, 
      interleukin (IL)-6, and monocyte chemoattractant protein (MCP)-1, as well as 
      phosphorylation of c-Jun N-terminal kinase/stress activated protein kinase 
      (JNK/SAPK), p38 mitogen-activated protein kinases (MAPK), and extracellular 
      signal-regulated kinases 1/2 (ERK1/2). These data demonstrate that XB exhibits 
      anti-diabetic, hypolipogenic, and anti-inflammatory effects via regulation of the 
      miR-122a/33a axis in db/db mice.
FAU - Lim, Eunjin
AU  - Lim E
AD  - Department of Nutritional Science and Food Management, Ewha Womans University, 
      Seoul 03760, Korea. leemeunjin@daum.net.
FAU - Lim, Ji Ye
AU  - Lim JY
AD  - Department of Nutritional Science and Food Management, Ewha Womans University, 
      Seoul 03760, Korea. godlovesme86@hanmail.net.
FAU - Kim, Eunju
AU  - Kim E
AD  - Department of Nutritional Science and Food Management, Ewha Womans University, 
      Seoul 03760, Korea. eunju831@naver.com.
FAU - Kim, Yoo-Sun
AU  - Kim YS
AD  - Department of Nutritional Science and Food Management, Ewha Womans University, 
      Seoul 03760, Korea. tidygirlss@naver.com.
FAU - Shin, Jae-Ho
AU  - Shin JH
AD  - Department of Biomedical Laboratory Science, Eulji University, Seongnam-si, 
      Gyunggi-do 13135, Korea. shinjh@eulji.ac.kr.
FAU - Seok, Pu Reum
AU  - Seok PR
AD  - Department of Biomedical Laboratory Science, Eulji University, Seongnam-si, 
      Gyunggi-do 13135, Korea. seok@eulji.ac.kr.
FAU - Jung, Sangwon
AU  - Jung S
AD  - R&D Center, TS Corporation, Incheon 22300, Korea. chemjsw@ts.co.kr.
FAU - Yoo, Sang-Ho
AU  - Yoo SH
AD  - Department of Food Science & Biotechnology, and Carbohydrate Bioproduct Research 
      Center, Sejong University, Seoul 05006, Korea. shyoo@sejong.ac.kr.
FAU - Kim, Yuri
AU  - Kim Y
AD  - Department of Nutritional Science and Food Management, Ewha Womans University, 
      Seoul 03760, Korea. yuri.kim@ewha.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20161205
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Disaccharides)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn122 microRNA, mouse)
RN  - 0 (Mirn33 microRNA, mouse)
RN  - 0 (Prebiotics)
RN  - 0 (Sweetening Agents)
RN  - ID02R0EG7P (xylobiose)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Experimental/metabolism/*therapy
MH  - Dietary Supplements
MH  - Disaccharides/*administration & dosage
MH  - Lipogenesis/genetics/*physiology
MH  - Liver/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*metabolism
MH  - Prebiotics/administration & dosage
MH  - Sweetening Agents/*administration & dosage
PMC - PMC5188446
OTO - NOTNLM
OT  - db/db mice
OT  - inflammation
OT  - lipogenesis
OT  - microRNA
OT  - xylobiose
COIS- The authors declare no conflict of interest.
EDAT- 2016/12/09 06:00
MHDA- 2017/04/04 06:00
PMCR- 2016/12/01
CRDT- 2016/12/09 06:00
PHST- 2016/09/04 00:00 [received]
PHST- 2016/11/23 00:00 [revised]
PHST- 2016/11/25 00:00 [accepted]
PHST- 2016/12/09 06:00 [entrez]
PHST- 2016/12/09 06:00 [pubmed]
PHST- 2017/04/04 06:00 [medline]
PHST- 2016/12/01 00:00 [pmc-release]
AID - nu8120791 [pii]
AID - nutrients-08-00791 [pii]
AID - 10.3390/nu8120791 [doi]
PST - epublish
SO  - Nutrients. 2016 Dec 5;8(12):791. doi: 10.3390/nu8120791.