PMID- 27930303
OWN - NLM
STAT- MEDLINE
DCOM- 20180413
LR  - 20181113
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 113
IP  - 51
DP  - 2016 Dec 20
TI  - Transcription factor Zeb2 regulates commitment to plasmacytoid dendritic cell and 
      monocyte fate.
PG  - 14775-14780
LID - 10.1073/pnas.1611408114 [doi]
AB  - Dendritic cells (DCs) and monocytes develop from a series of bone-marrow-resident 
      progenitors in which lineage potential is regulated by distinct transcription 
      factors. Zeb2 is an E-box-binding protein associated with epithelial-mesenchymal 
      transition and is widely expressed among hematopoietic lineages. Previously, we 
      observed that Zeb2 expression is differentially regulated in progenitors 
      committed to classical DC (cDC) subsets in vivo. Using systems for inducible gene 
      deletion, we uncover a requirement for Zeb2 in the development of Ly-6C(hi) 
      monocytes but not neutrophils, and we show a corresponding requirement for Zeb2 
      in expression of the M-CSF receptor in the bone marrow. In addition, we confirm a 
      requirement for Zeb2 in development of plasmacytoid DCs but find that Zeb2 is not 
      required for cDC2 development. Instead, Zeb2 may act to repress cDC1 progenitor 
      specification in the context of inflammatory signals.
FAU - Wu, Xiaodi
AU  - Wu X
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Briseno, Carlos G
AU  - Briseno CG
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Grajales-Reyes, Gary E
AU  - Grajales-Reyes GE
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Haldar, Malay
AU  - Haldar M
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Iwata, Arifumi
AU  - Iwata A
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Kretzer, Nicole M
AU  - Kretzer NM
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Kc, Wumesh
AU  - Kc W
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Tussiwand, Roxane
AU  - Tussiwand R
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Higashi, Yujiro
AU  - Higashi Y
AD  - Department of Perinatology, Institute for Developmental Research, Aichi Human 
      Service Center, Kasugai, Aichi 480-0392, Japan.
FAU - Murphy, Theresa L
AU  - Murphy TL
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110.
FAU - Murphy, Kenneth M
AU  - Murphy KM
AD  - Department of Pathology and Immunology, School of Medicine, Washington 
      University, St. Louis, MO 63110; kmurphy@wustl.edu.
AD  - Howard Hughes Medical Institute, School of Medicine, Washington University, St. 
      Louis, MO 63110.
LA  - eng
GR  - F31 CA189491/CA/NCI NIH HHS/United States
GR  - K08 AI106953/AI/NIAID NIH HHS/United States
GR  - P30 CA091842/CA/NCI NIH HHS/United States
GR  - UL1 TR000448/TR/NCATS NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20161205
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (ZEB2 protein, mouse)
RN  - 0 (Zinc Finger E-box Binding Homeobox 2)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Animals
MH  - Bone Marrow/metabolism
MH  - CD8-Positive T-Lymphocytes/cytology
MH  - Cell Lineage
MH  - Cytoplasm/metabolism
MH  - Dendritic Cells/*cytology
MH  - Female
MH  - Flow Cytometry
MH  - Gene Deletion
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Inflammation
MH  - Integrases/metabolism
MH  - Male
MH  - Mice
MH  - Monocytes/*cytology
MH  - Neutrophils/cytology/metabolism
MH  - Zinc Finger E-box Binding Homeobox 2/*genetics/*physiology
PMC - PMC5187668
OTO - NOTNLM
OT  - monocyte
OT  - plasmacytoid dendritic cell
OT  - transcription factor
COIS- The authors declare no conflict of interest.
EDAT- 2016/12/09 06:00
MHDA- 2018/04/14 06:00
PMCR- 2016/12/05
CRDT- 2016/12/09 06:00
PHST- 2016/12/09 06:00 [pubmed]
PHST- 2018/04/14 06:00 [medline]
PHST- 2016/12/09 06:00 [entrez]
PHST- 2016/12/05 00:00 [pmc-release]
AID - 1611408114 [pii]
AID - 201611408 [pii]
AID - 10.1073/pnas.1611408114 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14775-14780. doi: 
      10.1073/pnas.1611408114. Epub 2016 Dec 5.