PMID- 27931292
OWN - NLM
STAT- MEDLINE
DCOM- 20170220
LR  - 20190609
IS  - 1539-6304 (Electronic)
IS  - 1088-5412 (Linking)
VI  - 37
IP  - 6
DP  - 2016 Nov
TI  - Circulating follicular T-helper cell subset distribution in patients with asthma.
PG  - 154-161
AB  - BACKGROUND: The pathogenesis of allergic asthma is primarily characterized by 
      abnormality in the immunoglobulin E (IgE) pathway, suggesting a possible role for 
      follicular T-helper cells (Tfh) in the genesis of excessive IgE accumulation. The 
      blood chemokine (C-X-C motif) receptor 5 (CXCR5)+CD4+ T cells, known as 
      circulating Tfh, share common functional characteristics with Tfh cells from 
      germinal centers. There are three subsets of circulating Tfh cells: Tfh1 
      (CXCR3+CC chemokine receptor [CCR] 6), Tfh2 (CXCR3CCR6) and Tfh17 (CXCR3CCR6+). 
      However, data on circulating Tfh cell subsets distribution in patients with 
      asthma are not available. OBJECTIVE: To investigate the circulating Tfh cell 
      subsets distribution in patients with asthma and to assess the relationship 
      between Tfh cell subsets distribution and the serum IgE level. METHODS: Thirty 
      patients with severe allergic asthma and 30 age- and sex-matched healthy controls 
      were enrolled in this study. The percentages and phenotypic assays of circulating 
      Tfh cell subsets were assessed by flow cytometry. The total IgE levels were also 
      measured. The correlation between the percentage of circulating Tfh cell subsets 
      and the levels of serum total IgE was analyzed. RESULTS: Our results showed 
      polarization of Tfh2 cells within circulating Tfh cell subsets in the patients 
      with asthma. Phenotypic assays showed that activated Tfh2 subtypes displayed the 
      features of Tfh cells, including invariably coexpressed programmed cell death 1 
      (PD-1), and inducible costimulator (ICOS). Furthermore, not only the frequency of 
      Tfh2 cells but also the ratio (%Tfh2/%Tfh1) positively correlated with the total 
      IgE level in the blood. CONCLUSION: Results of our data described an altered 
      circulating Tfh subset distribution, which implied that this cell subset might 
      play an important role in the pathogenesis of asthma.
FAU - Gong, Fang
AU  - Gong F
AD  - Department of Laboratory Medicine, The Third Hospital Affiliated to Nantong 
      University, Wuxi, Jiangsu, P.R. China.
FAU - Qian, Chunyan
AU  - Qian C
FAU - Zhu, HuaYan
AU  - Zhu H
FAU - Zhu, Jie
AU  - Zhu J
FAU - Pan, Yuhong
AU  - Pan Y
FAU - Dong, QiaoJing
AU  - Dong Q
FAU - Jiang, DongLin
AU  - Jiang D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Allergy Asthma Proc
JT  - Allergy and asthma proceedings
JID - 9603640
RN  - 0 (Anti-Asthmatic Agents)
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Anti-Asthmatic Agents/administration & dosage
MH  - Antigens, Surface/metabolism
MH  - Asthma/*blood/diagnosis/drug therapy/*immunology
MH  - Biomarkers
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood/immunology
MH  - Immunophenotyping
MH  - *Lymphocyte Count
MH  - Male
MH  - Middle Aged
MH  - Respiratory Function Tests
MH  - T-Lymphocyte Subsets/*immunology/metabolism
MH  - T-Lymphocytes, Helper-Inducer/*immunology/metabolism
EDAT- 2016/12/10 06:00
MHDA- 2017/08/18 06:00
CRDT- 2016/12/10 06:00
PHST- 2016/12/10 06:00 [entrez]
PHST- 2016/12/10 06:00 [pubmed]
PHST- 2017/08/18 06:00 [medline]
AID - 10.2500/aap.2016.37.3982 [doi]
PST - ppublish
SO  - Allergy Asthma Proc. 2016 Nov;37(6):154-161. doi: 10.2500/aap.2016.37.3982.