PMID- 27932223 OWN - NLM STAT- MEDLINE DCOM- 20170707 LR - 20220409 IS - 1549-4713 (Electronic) IS - 0161-6420 (Linking) VI - 124 IP - 3 DP - 2017 Mar TI - Relationship between Psychophysical Measures of Retinal Ganglion Cell Density and In Vivo Measures of Cone Density in Glaucoma. PG - 310-319 LID - S0161-6420(16)31809-7 [pii] LID - 10.1016/j.ophtha.2016.10.029 [doi] AB - PURPOSE: Considerable between-individual variation in retinal ganglion cell (RGC) density exists in healthy individuals, making identification of change from normal to glaucoma difficult. In ascertaining local cone-to-RGC density ratios in healthy individuals, we wished to investigate the usefulness of objective cone density estimates as a surrogate of baseline RGC density in glaucoma patients, and thus a more efficient way of identifying early changes. DESIGN: Exploratory cohort study. PARTICIPANTS: Twenty glaucoma patients (60% women) with a median age of 54 years and mean deviation (MD) in the visual field of -5 dB and 20 healthy controls (70% women) with a median age of 57 years and a mean MD of 0 dB were included. METHODS: Glaucoma patients and healthy participants underwent in vivo cone imaging at 4 locations of 8.8 degrees eccentricity with a modified Heidelberg Retina Angiograph HRA2 (scan angle, 3 degrees ). Cones were counted using an automated program. Retinal ganglion cell density was estimated at the same test locations from peripheral grating resolution acuity thresholds. MAIN OUTCOME MEASURES: Retinal cone density, estimated RGC density, and cone-to-RGC ratios in glaucoma patients and healthy controls. RESULTS: Median cone-to-RGC density was 3.51:1 (interquartile range [IQR], 2.59:1-6.81:1) in glaucoma patients compared with 2.35:1 (IQR, 1.83:1-2.82:1) in healthy participants. Retinal ganglion cell density was 33% lower in glaucoma patients than in healthy participants; however, cone density was very similar in glaucoma patients (7248 cells/mm(2)) and healthy controls (7242 cells/mm(2)). The area under the receiver operator characteristic curve was 0.79 (95% confidence interval [CI], 0.71-0.86) for both RGC density and cone-to-RGC ratio and 0.49 (95% CI, 0.39-0.58) for cone density. CONCLUSIONS: Local measurements of cone density do not differ significantly from normal in glaucoma patients despite large differences in RGC density. There was no statistically significant association between RGC density and cone density in the normal participants, and the range of cone-to-RGC density ratios was relatively large in healthy controls. These findings suggest that estimates of baseline RGC density from cone density are unlikely to be precise and offer little advantage over determination of RGC alone in the identification of early glaucomatous change. CI - Copyright (c) 2016 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved. FAU - Matlach, Juliane AU - Matlach J AD - National Institute for Health Research Biomedical Research Centre, Moorfields Eye Hospital and University College London Institute of Ophthalmology, London, United Kingdom; Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany. FAU - Mulholland, Padraig J AU - Mulholland PJ AD - National Institute for Health Research Biomedical Research Centre, Moorfields Eye Hospital and University College London Institute of Ophthalmology, London, United Kingdom; Vision Science Research Group, Ulster University, Coleraine, United Kingdom. FAU - Cilkova, Marketa AU - Cilkova M AD - National Institute for Health Research Biomedical Research Centre, Moorfields Eye Hospital and University College London Institute of Ophthalmology, London, United Kingdom. FAU - Chopra, Reena AU - Chopra R AD - National Institute for Health Research Biomedical Research Centre, Moorfields Eye Hospital and University College London Institute of Ophthalmology, London, United Kingdom. FAU - Shah, Nilpa AU - Shah N AD - National Institute for Health Research Biomedical Research Centre, Moorfields Eye Hospital and University College London Institute of Ophthalmology, London, United Kingdom. FAU - Redmond, Tony AU - Redmond T AD - School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom. FAU - Dakin, Steven C AU - Dakin SC AD - Department of Optometry & Vision Science, University of Auckland, Auckland, New Zealand. FAU - Garway-Heath, David F AU - Garway-Heath DF AD - National Institute for Health Research Biomedical Research Centre, Moorfields Eye Hospital and University College London Institute of Ophthalmology, London, United Kingdom. FAU - Anderson, Roger S AU - Anderson RS AD - National Institute for Health Research Biomedical Research Centre, Moorfields Eye Hospital and University College London Institute of Ophthalmology, London, United Kingdom; Vision Science Research Group, Ulster University, Coleraine, United Kingdom. Electronic address: rs.anderson@ulster.ac.uk. LA - eng GR - II-LA-0813-20004/DH_/Department of Health/United Kingdom PT - Journal Article DEP - 20161205 PL - United States TA - Ophthalmology JT - Ophthalmology JID - 7802443 SB - IM MH - Cell Count MH - Cohort Studies MH - Female MH - Glaucoma, Open-Angle/*diagnosis/physiopathology MH - Healthy Volunteers MH - Humans MH - Intraocular Pressure/physiology MH - Male MH - Middle Aged MH - Optic Nerve Diseases/diagnosis/physiopathology MH - Psychophysics MH - Retinal Cone Photoreceptor Cells/*pathology MH - Retinal Ganglion Cells/*pathology MH - Tomography, Optical Coherence MH - Visual Field Tests MH - Visual Fields/physiology EDAT- 2016/12/10 06:00 MHDA- 2017/07/08 06:00 CRDT- 2016/12/10 06:00 PHST- 2016/04/24 00:00 [received] PHST- 2016/10/25 00:00 [revised] PHST- 2016/10/26 00:00 [accepted] PHST- 2016/12/10 06:00 [pubmed] PHST- 2017/07/08 06:00 [medline] PHST- 2016/12/10 06:00 [entrez] AID - S0161-6420(16)31809-7 [pii] AID - 10.1016/j.ophtha.2016.10.029 [doi] PST - ppublish SO - Ophthalmology. 2017 Mar;124(3):310-319. doi: 10.1016/j.ophtha.2016.10.029. Epub 2016 Dec 5.