PMID- 27932267
OWN - NLM
STAT- MEDLINE
DCOM- 20171026
LR  - 20180917
IS  - 1874-9399 (Print)
IS  - 1874-9399 (Linking)
VI  - 1860
IP  - 2
DP  - 2017 Feb
TI  - Human PRE-PIK3C2B, an intronic cis-element with dual function of activation and 
      repression.
PG  - 196-204
LID - S1874-9399(16)30277-2 [pii]
LID - 10.1016/j.bbagrm.2016.12.003 [doi]
AB  - The Polycomb/Trithorax Responsive Elements (PRE/TREs) are the cis-regulatory 
      sequences that interact with both repressive (PcG) as well as activating (TrxG) 
      complexes. However, most of the mammalian PREs are demonstrated to interact with 
      the repressive polycomb (PcG) complexes only. We have carried out an unbiased 
      search for proteins interacting with human PRE-PIK3C2B (hPRE-PIK3C2B) based on 
      DNA affinity purification followed by mass spectrometry and identified MLL, MLL4 
      and WDR87 among other proteins in three biological replicates in HEK, U87 and 
      HeLa cell lines. The hPRE-PIK3C2B interacts with the members of multiple 
      activating complexes (COMPASS-like). The increase in the interaction of MLL and 
      MLL4 on depletion of YY1 and the increase in the enrichment of YY1 and EZH2 upon 
      MLL knockdown at the hPRE-PIK3C2B indicate the dual occupancy and suggest a 
      concentration dependent enrichment of the activator or the repressor complex at 
      hPRE-PIK3C2B. Further, we show that the hPRE-PIK3C2B interacts with the 
      Drosophila homologues of PcG and TrxG proteins in transgenic flies. Here, we 
      found that there is an increased enrichment of Pc (Polycomb) in comparison to Trx 
      (TrxG protein) at hPRE-PIK3C2B in the Drosophila transgenic flies and this seems 
      to be the default state while the balance is tipped towards the trithorax complex 
      in PcG mutants. To the best of our knowledge, this is one of the early 
      demonstrations of human PRE acting as a TRE without any sequence alteration.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Maini, Jayant
AU  - Maini J
AD  - Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 
      110007, India.
FAU - Ghasemi, Mohsen
AU  - Ghasemi M
AD  - Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 
      110007, India.
FAU - Yandhuri, Deepti
AU  - Yandhuri D
AD  - CSIR-Centre for Cellular & Molecular Biology, Uppal Road, Habsiguda, Hyderabad 
      500007, India.
FAU - Thakur, Suman S
AU  - Thakur SS
AD  - CSIR-Centre for Cellular & Molecular Biology, Uppal Road, Habsiguda, Hyderabad 
      500007, India.
FAU - Brahmachari, Vani
AU  - Brahmachari V
AD  - Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 
      110007, India. Electronic address: vani.brahmachari@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161206
PL  - Netherlands
TA  - Biochim Biophys Acta Gene Regul Mech
JT  - Biochimica et biophysica acta. Gene regulatory mechanisms
JID - 101731723
RN  - 0 (Polycomb-Group Proteins)
RN  - 0 (YY1 Transcription Factor)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 1.8.1.9 (Thioredoxin Reductase 1)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.7.1.137 (Class II Phosphatidylinositol 3-Kinases)
SB  - IM
EIN - Biochim Biophys Acta. 2017 Jun;1860(6):750. PMID: 28371618
MH  - Animals
MH  - Animals, Genetically Modified/genetics
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Class II Phosphatidylinositol 3-Kinases/*genetics
MH  - Drosophila melanogaster/genetics
MH  - Enhancer of Zeste Homolog 2 Protein/genetics
MH  - Epigenetic Repression/*genetics
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Histone-Lysine N-Methyltransferase/genetics
MH  - Humans
MH  - Introns/*genetics
MH  - Myeloid-Lymphoid Leukemia Protein/genetics
MH  - Polycomb-Group Proteins/*genetics
MH  - Thioredoxin Reductase 1/genetics
MH  - Transcriptional Activation/*genetics
MH  - YY1 Transcription Factor/genetics
OTO - NOTNLM
OT  - Chromatin interaction
OT  - Genetic interaction
OT  - Human PRE-TRE
OT  - MLL
OT  - Polycomb and trithorax
OT  - Regulation
EDAT- 2016/12/10 06:00
MHDA- 2017/10/27 06:00
CRDT- 2016/12/10 06:00
PHST- 2016/07/02 00:00 [received]
PHST- 2016/10/28 00:00 [revised]
PHST- 2016/12/02 00:00 [accepted]
PHST- 2016/12/10 06:00 [pubmed]
PHST- 2017/10/27 06:00 [medline]
PHST- 2016/12/10 06:00 [entrez]
AID - S1874-9399(16)30277-2 [pii]
AID - 10.1016/j.bbagrm.2016.12.003 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Gene Regul Mech. 2017 Feb;1860(2):196-204. doi: 
      10.1016/j.bbagrm.2016.12.003. Epub 2016 Dec 6.