PMID- 27934830
OWN - NLM
STAT- MEDLINE
DCOM- 20170217
LR  - 20170817
IS  - 1998-3646 (Electronic)
IS  - 0255-0857 (Linking)
VI  - 34
IP  - 4
DP  - 2016 Oct-Dec
TI  - Coagulase-negative staphylococci causing blood stream infection at an Indian 
      tertiary care hospital: Prevalence, antimicrobial resistance and molecular 
      characterisation.
PG  - 500-505
LID - 10.4103/0255-0857.195374 [doi]
AB  - INTRODUCTION: Recent years have seen a rise of coagulase-negative staphylococci 
      (CoNS) from common contaminants to agents of nosocomial blood stream infections 
      (BSI's). Molecular typing and establishing a correlation with antibiotic 
      resistance is essential particularly in countries like India where genotyping 
      studies for drug-resistant CoNS are sparse. METHODS: A prospective study was done 
      over 18 months, wherein 42,693 blood samples were received, and 59 patients with 
      BSI due to CoNS were evaluated. The isolates recovered were identified by a 
      biochemical test panel and matrix-assisted laser desorption ionization - time of 
      flight mass spectrometry followed by antimicrobial susceptibility testing by 
      Kirby-Baur disc diffusion method and E-test strips. Staphylococcal chromosomal 
      cassette mec (SCCmec) element was characterised by multiplex polymerase chain 
      reaction for all methicillin-resistant (MR) isolates. RESULTS: The majority of 
      CoNS isolated were constituted by Staphylococcus haemolyticus (47.5%) followed by 
      Staphylococcus epidermidis (33.9%), Staphylococcus hominis (11.86%), 
      Staphylococcus cohnii (5.08%) and Staphylococcus warneri (1.69%). Among all 
      isolates 57.6% were MR with statistically significant higher resistance versus 
      methicillin sensitive-CoNS. This difference was significant for erythromycin (76% 
      vs. 44%, P = 0.011), rifampicin (50% vs. 12%,P= 0.002) and amikacin (26.5% vs. 
      4%, P = 0.023), ciprofloxacin (64.7% vs. 20%, P = 0.001) and cotrimoxazole (55.9% 
      vs. 20%, P = 0.006). SCCmec type I was predominant (61.8%, P = 0.028) and 
      exhibited multidrug resistance (76.2%). Coexistence of SCCmec type I and III was 
      seen in 8.82% MR isolates. CONCLUSION: CoNS exhibit high antimicrobial resistance 
      thereby limiting treatment options. The presence of new variants of SCCmec type 
      in hospital-acquired CoNS may predict the antibiotic resistance pattern. This is 
      the first evaluation of the molecular epidemiology of CoNS causing BSI from India 
      and can serve as a guide in the formulation of hospital infection control and 
      treatment guidelines.
FAU - Singh, S
AU  - Singh S
AD  - Department of Medical Microbiology, Post Graduate Institute of Medical Education 
      and Research, Chandigarh, India.
FAU - Dhawan, B
AU  - Dhawan B
AD  - Department of Microbiology, All Institute of Medical Sciences, New Delhi, India.
FAU - Kapil, A
AU  - Kapil A
AD  - Department of Microbiology, All Institute of Medical Sciences, New Delhi, India.
FAU - Kabra, S K
AU  - Kabra SK
AD  - Department of Pediatrics, All Institute of Medical Sciences, New Delhi, India.
FAU - Suri, A
AU  - Suri A
AD  - Department of Neurosurgery, All Institute of Medical Sciences, New Delhi, India.
FAU - Sreenivas, V
AU  - Sreenivas V
AD  - Department of Biostatistics, All Institute of Medical Sciences, New Delhi, India.
FAU - Das, B K
AU  - Das BK
AD  - Department of Microbiology, All Institute of Medical Sciences, New Delhi, India.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Indian J Med Microbiol
JT  - Indian journal of medical microbiology
JID - 8700903
RN  - 0 (Coagulase)
SB  - IM
MH  - Bacteremia/*epidemiology/microbiology
MH  - Bacterial Typing Techniques
MH  - Coagulase/*analysis
MH  - Cross Infection/*epidemiology/microbiology
MH  - *Drug Resistance, Bacterial
MH  - Genotype
MH  - Humans
MH  - India/epidemiology
MH  - Microbial Sensitivity Tests
MH  - *Molecular Typing
MH  - Prevalence
MH  - Prospective Studies
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Staphylococcal Infections/*epidemiology/microbiology
MH  - Staphylococcus/classification/drug effects/genetics/*isolation & purification
MH  - Staphylococcus epidermidis
MH  - Staphylococcus haemolyticus
MH  - Staphylococcus hominis
MH  - Tertiary Care Centers
EDAT- 2016/12/10 06:00
MHDA- 2017/02/18 06:00
CRDT- 2016/12/10 06:00
PHST- 2016/12/10 06:00 [entrez]
PHST- 2016/12/10 06:00 [pubmed]
PHST- 2017/02/18 06:00 [medline]
AID - IndianJMedMicrobiol_2016_34_4_500_195374 [pii]
AID - 10.4103/0255-0857.195374 [doi]
PST - ppublish
SO  - Indian J Med Microbiol. 2016 Oct-Dec;34(4):500-505. doi: 
      10.4103/0255-0857.195374.