PMID- 27936052
OWN - NLM
STAT- MEDLINE
DCOM- 20170707
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 12
DP  - 2016
TI  - MicroRNA-182 Suppresses HGF/SF-Induced Increases in Retinal Pigment Epithelial 
      Cell Proliferation and Migration through Targeting c-Met.
PG  - e0167684
LID - 10.1371/journal.pone.0167684 [doi]
LID - e0167684
AB  - As increases in hepatocyte growth factor/scatter factor (HGF/SF) induce retinal 
      pigment epithelial (RPE) migration and proliferation into the vitreous cavity and 
      contribute to proliferative vitreoretinopathy (PVR) development, we determined if 
      changes in miR-182 expression affect such behavioral changes. We found that 
      miR-182 expression was less in PVR clinical samples than in primary RPE cells 
      whereas c-Met was upregulated. Ectopic miR-182 inhibited RPE cell proliferation, 
      cell cycle, and migration. Bioinformatic analysis identified c-Met as a miR-182 
      target, which was confirmed with the luciferase reporter assay. Transfection of 
      miR-182 into RPE cells induced c-Met downregulation, which led to reduced cell 
      proliferation and migration through declines in p-Akt formation. MiR-182 
      downregulation along with c-Met upregulation in PVR tissues suggest that these 
      two opposing effects play important roles in PVR development. As ectopic miR-182 
      expression suppressed RPE cell proliferation and migration, strategies to 
      selectively upregulate miR-182 expression in a clinical setting may provide a 
      novel option to treat this disease.
FAU - Wang, Lihua
AU  - Wang L
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, 
      Wenzhou, Zhejiang, China.
AD  - State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, 
      Ministry of Health of the People's Republic of China, Zhejiang Provincial Key 
      Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
FAU - Dong, Feng
AU  - Dong F
AD  - The First Affiliated Hospital of College of Medicine, Zhejiang University, 
      Hangzhou, Zhejiang, China.
FAU - Reinach, Peter S
AU  - Reinach PS
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, 
      Wenzhou, Zhejiang, China.
AD  - State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, 
      Ministry of Health of the People's Republic of China, Zhejiang Provincial Key 
      Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
FAU - He, Dandan
AU  - He D
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, 
      Wenzhou, Zhejiang, China.
AD  - State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, 
      Ministry of Health of the People's Republic of China, Zhejiang Provincial Key 
      Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
FAU - Zhao, Xiaoting
AU  - Zhao X
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, 
      Wenzhou, Zhejiang, China.
AD  - State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, 
      Ministry of Health of the People's Republic of China, Zhejiang Provincial Key 
      Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
FAU - Chen, Xiaoyan
AU  - Chen X
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, 
      Wenzhou, Zhejiang, China.
AD  - State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, 
      Ministry of Health of the People's Republic of China, Zhejiang Provincial Key 
      Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
FAU - Hu, Dan-Ning
AU  - Hu DN
AD  - State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, 
      Ministry of Health of the People's Republic of China, Zhejiang Provincial Key 
      Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
AD  - Tissue Culture Center, the New York Eye and Ear Infirmary, New York Medical 
      College, New York, New York, United States of America.
FAU - Yan, Dongsheng
AU  - Yan D
AUID- ORCID: 0000-0003-0551-2219
AD  - School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, 
      Wenzhou, Zhejiang, China.
AD  - State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, 
      Ministry of Health of the People's Republic of China, Zhejiang Provincial Key 
      Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
LA  - eng
PT  - Journal Article
DEP - 20161209
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (HGF protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn182 microRNA, human)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (MET protein, human)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Adult
MH  - Cell Movement
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - *Gene Expression Regulation
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Proto-Oncogene Proteins c-met/*genetics
MH  - Retinal Pigment Epithelium/cytology/metabolism/*pathology
MH  - Up-Regulation
MH  - Vitreoretinopathy, Proliferative/*genetics/metabolism/pathology
PMC - PMC5147940
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/12/10 06:00
MHDA- 2017/07/08 06:00
PMCR- 2016/12/09
CRDT- 2016/12/10 06:00
PHST- 2016/01/18 00:00 [received]
PHST- 2016/11/18 00:00 [accepted]
PHST- 2016/12/10 06:00 [entrez]
PHST- 2016/12/10 06:00 [pubmed]
PHST- 2017/07/08 06:00 [medline]
PHST- 2016/12/09 00:00 [pmc-release]
AID - PONE-D-16-02329 [pii]
AID - 10.1371/journal.pone.0167684 [doi]
PST - epublish
SO  - PLoS One. 2016 Dec 9;11(12):e0167684. doi: 10.1371/journal.pone.0167684. 
      eCollection 2016.