PMID- 27936166
OWN - NLM
STAT- MEDLINE
DCOM- 20170710
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 12
DP  - 2016
TI  - Increased Age, but Not Parity Predisposes to Higher Bacteriuria Burdens Due to 
      Streptococcus Urinary Tract Infection and Influences Bladder Cytokine Responses, 
      Which Develop Independent of Tissue Bacterial Loads.
PG  - e0167732
LID - 10.1371/journal.pone.0167732 [doi]
LID - e0167732
AB  - Streptococcus agalactiae causes urinary tract infection (UTI) in pregnant adults, 
      non-pregnant adults, immune-compromised individuals and the elderly. The 
      pathogenesis of S. agalactiae UTI in distinct patient populations is poorly 
      understood. In this study, we used murine models of UTI incorporating young mice, 
      aged and dam mice to show that uropathogenic S. agalactiae causes bacteriuria at 
      significantly higher levels in aged mice compared to young mice and this occurs 
      coincident with equivalent levels of bladder tissue colonisation at 24 h 
      post-infection (p.i.). In addition, aged mice exhibited significantly higher 
      bacteriuria burdens at 48 h compared to young mice, confirming a divergent 
      pattern of bacterial colonization in the urinary tract of aged and young mice. 
      Multiparous mice, in contrast, exhibited significantly lower urinary titres of S. 
      agalactiae compared to age-matched nulliparous mice suggesting that parity 
      enhances the ability of the host to control S. agalactiae bacteriuria. 
      Additionally, we show that both age and parity alter the expression levels of 
      several key regulatory and pro-inflammatory cytokines, which are known to be 
      important the immune response to UTI, including Interleukin (IL)-1beta, IL-12(p40), 
      and Monocyte Chemoattractant Protein-1 (MCP-1). Finally, we demonstrate that 
      other cytokines, including IL-17 are induced significantly in the S. 
      agalactiae-infected bladder regardless of age and parity status. Collectively, 
      these findings show that the host environment plays an important role in 
      influencing the severity of S. agalactiae UTI; infection dynamics, particularly 
      in the context of bacteriuria, depend on age and parity, which also affect the 
      nature of innate immune responses to infection.
FAU - Sullivan, Matthew J
AU  - Sullivan MJ
AD  - School of Medical Science, and Menzies Health Institute Queensland, Griffith 
      University, Gold Coast, Queensland, Australia.
FAU - Carey, Alison J
AU  - Carey AJ
AD  - School of Medical Science, and Menzies Health Institute Queensland, Griffith 
      University, Gold Coast, Queensland, Australia.
FAU - Leclercq, Sophie Y
AU  - Leclercq SY
AD  - School of Medical Science, and Menzies Health Institute Queensland, Griffith 
      University, Gold Coast, Queensland, Australia.
AD  - Research and Development Center, Ezequiel Dias Foundation (Funed), Belo 
      Horizonte, MG, Brazil.
FAU - Tan, Chee K
AU  - Tan CK
AD  - School of Medical Science, and Menzies Health Institute Queensland, Griffith 
      University, Gold Coast, Queensland, Australia.
FAU - Ulett, Glen C
AU  - Ulett GC
AUID- ORCID: 0000-0002-9638-8091
AD  - School of Medical Science, and Menzies Health Institute Queensland, Griffith 
      University, Gold Coast, Queensland, Australia.
LA  - eng
PT  - Journal Article
DEP - 20161209
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cytokines)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Bacterial Load
MH  - Bacteriuria/*epidemiology/immunology/microbiology/pathology
MH  - Cytokines/analysis/immunology
MH  - Female
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Parity
MH  - Pregnancy
MH  - Streptococcal Infections/*epidemiology/immunology/microbiology/pathology
MH  - Streptococcus agalactiae/immunology/*isolation & purification
MH  - Urinary Bladder/immunology/*microbiology/pathology
MH  - Urinary Tract Infections/*epidemiology/immunology/microbiology/pathology
PMC - PMC5147962
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/12/10 06:00
MHDA- 2017/07/14 06:00
PMCR- 2016/12/09
CRDT- 2016/12/10 06:00
PHST- 2016/06/16 00:00 [received]
PHST- 2016/11/18 00:00 [accepted]
PHST- 2016/12/10 06:00 [entrez]
PHST- 2016/12/10 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2016/12/09 00:00 [pmc-release]
AID - PONE-D-16-24137 [pii]
AID - 10.1371/journal.pone.0167732 [doi]
PST - epublish
SO  - PLoS One. 2016 Dec 9;11(12):e0167732. doi: 10.1371/journal.pone.0167732. 
      eCollection 2016.