PMID- 27936205
OWN - NLM
STAT- MEDLINE
DCOM- 20170710
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 12
DP  - 2016
TI  - Hyperhomocysteinemia in ApoE-/- Mice Leads to Overexpression of Enhancer of Zeste 
      Homolog 2 via miR-92a Regulation.
PG  - e0167744
LID - 10.1371/journal.pone.0167744 [doi]
LID - e0167744
AB  - Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular 
      diseases, such as atherosclerosis. HHcy promotes atherogenesis by modifying the 
      histone methylation patterns and miRNA regulation. In this study, we investigated 
      the effects of homocysteine (Hcy) on the expression of enhancer of zeste homolog 
      2 (EZH2), and tested our hypothesis that Hcy-induced atherosclerosis is mediated 
      by increased EZH2 expression, which is regulated by miR-92a. The levels of EZH2 
      and H3K27me3 were increased in the aorta of ApoE-/- mice fed a high-methionine 
      diet for 16 weeks, whereas miR-92a expression was decreased. Over-expression of 
      EZH2 increased H3K27me3 level and the accumulation of total cholesterol and 
      triglycerides in the foam cells. Furthermore, upregulation of miR-92a reduced 
      EZH2 expression in the foam cells. These data suggested that EZH2 plays a key 
      role in Hcy-mediated lipid metabolism disorders, and that miR-92a may be a novel 
      therapeutic target in Hcy-related atherosclerosis.
FAU - Xiaoling, Yang
AU  - Xiaoling Y
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
AD  - Institution of Medical Science of Ningxia, Yinchuan, Ningxia, China.
FAU - Li, Zhao
AU  - Li Z
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - ShuQiang, Li
AU  - ShuQiang L
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Shengchao, Ma
AU  - Shengchao M
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Anning, Yang
AU  - Anning Y
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Ning, Ding
AU  - Ning D
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Nan, Li
AU  - Nan L
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Yuexia, Jia
AU  - Yuexia J
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Xiaoming, Yang
AU  - Xiaoming Y
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Guizhong, Li
AU  - Guizhong L
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
FAU - Yideng, Jiang
AU  - Yideng J
AD  - Basic Medical School, Ningxia Medical University, Key Laboratory of 
      Cardio-Cerebro-Vascular Diseases, Ningxia Medical University, Yinchuan, Ningxia, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20161209
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Apolipoproteins E)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn92 microRNA, mouse)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
SB  - IM
EIN - PLoS One. 2020 Oct 12;15(10):e0240762. PMID: 33045026
MH  - Animals
MH  - Apolipoproteins E/*genetics
MH  - Atherosclerosis/etiology/genetics/metabolism
MH  - Cell Line
MH  - Down-Regulation
MH  - Enhancer of Zeste Homolog 2 Protein/*genetics/metabolism
MH  - Gene Deletion
MH  - Homocysteine/metabolism
MH  - Humans
MH  - Hyperhomocysteinemia/complications/*genetics/metabolism
MH  - Lipid Metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics/metabolism
MH  - *Up-Regulation
PMC - PMC5147974
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/12/10 06:00
MHDA- 2017/07/14 06:00
PMCR- 2016/12/09
CRDT- 2016/12/10 06:00
PHST- 2016/07/05 00:00 [received]
PHST- 2016/11/19 00:00 [accepted]
PHST- 2016/12/10 06:00 [entrez]
PHST- 2016/12/10 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2016/12/09 00:00 [pmc-release]
AID - PONE-D-16-26886 [pii]
AID - 10.1371/journal.pone.0167744 [doi]
PST - epublish
SO  - PLoS One. 2016 Dec 9;11(12):e0167744. doi: 10.1371/journal.pone.0167744. 
      eCollection 2016.