PMID- 27936653
OWN - NLM
STAT- MEDLINE
DCOM- 20170825
LR  - 20170825
IS  - 1520-5126 (Electronic)
IS  - 0002-7863 (Linking)
VI  - 139
IP  - 1
DP  - 2017 Jan 11
TI  - Identifying Glyceraldehyde 3-Phosphate Dehydrogenase as a Cyclic Adenosine 
      Diphosphoribose Binding Protein by Photoaffinity Protein-Ligand Labeling 
      Approach.
PG  - 156-170
LID - 10.1021/jacs.6b08088 [doi]
AB  - Cyclic adenosine diphosphoribose (cADPR), an endogenous nucleotide derived from 
      nicotinamide adenine dinucleotide (NAD(+)), mobilizes Ca(2+) release from 
      endoplasmic reticulum (ER) via ryanodine receptors (RyRs), yet the bridging 
      protein(s) between cADPR and RyRs remain(s) unknown. Here we synthesized a novel 
      photoaffinity labeling (PAL) cADPR agonist, PAL-cIDPRE, and subsequently applied 
      it to purify its binding proteins in human Jurkat T cells. We identified 
      glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as one of the cADPR binding 
      protein(s), characterized the binding affinity between cADPR and GAPDH in vitro 
      by surface plasmon resonance (SPR) assay, and mapped cADPR's binding sites in 
      GAPDH. We further demonstrated that cADPR induces the transient interaction 
      between GAPDH and RyRs in vivo and that GAPDH knockdown abolished cADPR-induced 
      Ca(2+) release. However, GAPDH did not catalyze cADPR into any other known or 
      novel compound(s). In summary, our data clearly indicate that GAPDH is the 
      long-sought-after cADPR binding protein and is required for cADPR-mediated Ca(2+) 
      mobilization from ER via RyRs.
FAU - Zhang, Kehui
AU  - Zhang K
AD  - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical 
      Sciences, Peking University , Beijing 100191, China.
AD  - Department of Biomedical Sciences, City University of Hong Kong , Hong Kong, 
      China.
FAU - Sun, Wei
AU  - Sun W
AD  - Department of Biomedical Sciences, City University of Hong Kong , Hong Kong, 
      China.
AD  - Department of Biology and Shenzhen Key Laboratory of Cell Microenvironment, South 
      University of Science and Technology of China , Shenzhen 518052, China.
FAU - Huang, Lihong
AU  - Huang L
AD  - Department of Biomedical Sciences, City University of Hong Kong , Hong Kong, 
      China.
FAU - Zhu, Kaiyuan
AU  - Zhu K
AD  - Department of Biomedical Sciences, City University of Hong Kong , Hong Kong, 
      China.
FAU - Pei, Fen
AU  - Pei F
AD  - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical 
      Sciences, Peking University , Beijing 100191, China.
FAU - Zhu, Longchao
AU  - Zhu L
AD  - Department of Biomedical Sciences, City University of Hong Kong , Hong Kong, 
      China.
FAU - Wang, Qian
AU  - Wang Q
AD  - Department of Biomedical Sciences, City University of Hong Kong , Hong Kong, 
      China.
FAU - Lu, Yingying
AU  - Lu Y
AD  - Department of Biomedical Sciences, City University of Hong Kong , Hong Kong, 
      China.
FAU - Zhang, Hongmin
AU  - Zhang H
AD  - Department of Biology and Shenzhen Key Laboratory of Cell Microenvironment, South 
      University of Science and Technology of China , Shenzhen 518052, China.
FAU - Jin, Hongwei
AU  - Jin H
AD  - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical 
      Sciences, Peking University , Beijing 100191, China.
FAU - Zhang, Li-He
AU  - Zhang LH
AD  - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical 
      Sciences, Peking University , Beijing 100191, China.
FAU - Zhang, Liangren
AU  - Zhang L
AD  - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical 
      Sciences, Peking University , Beijing 100191, China.
FAU - Yue, Jianbo
AU  - Yue J
AUID- ORCID: 0000-0001-6384-5447
AD  - Department of Biomedical Sciences, City University of Hong Kong , Hong Kong, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20161223
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Carrier Proteins)
RN  - 0 (Ligands)
RN  - 0 (Photoaffinity Labels)
RN  - 20762-30-5 (Adenosine Diphosphate Ribose)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Adenosine Diphosphate Ribose/chemistry/*metabolism
MH  - Carrier Proteins/chemistry/*metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/chemistry/genetics/*metabolism
MH  - Humans
MH  - Jurkat Cells
MH  - Ligands
MH  - Models, Molecular
MH  - Molecular Conformation
MH  - Photoaffinity Labels/chemistry/*metabolism
EDAT- 2016/12/13 06:00
MHDA- 2017/08/26 06:00
CRDT- 2016/12/13 06:00
PHST- 2016/12/13 06:00 [pubmed]
PHST- 2017/08/26 06:00 [medline]
PHST- 2016/12/13 06:00 [entrez]
AID - 10.1021/jacs.6b08088 [doi]
PST - ppublish
SO  - J Am Chem Soc. 2017 Jan 11;139(1):156-170. doi: 10.1021/jacs.6b08088. Epub 2016 
      Dec 23.