PMID- 27937753
OWN - NLM
STAT- MEDLINE
DCOM- 20170918
LR  - 20181202
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 26
IP  - 6
DP  - 2017 Mar 15
TI  - Isolation and Characterization of Multipotent Mesenchymal Stem Cells Adhering to 
      Adipocytes in Canine Bone Marrow.
PG  - 431-440
LID - 10.1089/scd.2016.0200 [doi]
AB  - The ceiling culture method has been used to isolate mature adipocytes from 
      adipose tissue that can be dedifferentiated into fibroblastic cells, also known 
      as dedifferentiated fat (DFAT) cells that self-renew and are multipotent, with 
      much higher homogeneity and colony-forming efficiency than those of adipose 
      tissue-derived mesenchymal stem cells. We cultured adipocytes from canine bone 
      marrow using this technique, with the expectation of obtaining DFAT cells. 
      However, contrary to our expectations, continuous monitoring of ceiling cultures 
      by time-lapse microscopy revealed many small cells adhering to adipocytes that 
      proliferated rapidly into cells with a fibroblastic morphology and without any 
      dedifferentiation from adipocytes. We named these cells bone marrow 
      peri-adipocyte cells (BM-PACs) and demonstrated the multipotent properties of 
      BM-PACs compared to that of conventionally cultured canine bone marrow 
      mesenchymal stem cells (BMMSCs). BM-PACs showed significantly greater 
      clonogenicity and proliferation ability than BMMSCs. An in vitro trilineage 
      differentiation assay revealed that BM-PACs possess adipogenic, osteogenic, and 
      chondrogenic capacities superior to those of BMMSCs. Flow cytometric analysis 
      revealed that the expression of CD73, which plays an important role in cell 
      growth and differentiation, was significantly higher in BM-PACs than in BMMSCs. 
      These results indicate that canine BM-PACs have stem cell characteristics that 
      are superior to those of BMMSCs, and that these mesenchymal stem cells (MSCs) 
      appear to be a feasible source for cell-based therapies in dogs.
FAU - Lin, Hsing-Yi
AU  - Lin HY
AD  - Laboratory of Veterinary Surgery, Graduate School of Agriculture and Life 
      Sciences, The University of Tokyo , Tokyo, Japan .
FAU - Fujita, Naoki
AU  - Fujita N
AD  - Laboratory of Veterinary Surgery, Graduate School of Agriculture and Life 
      Sciences, The University of Tokyo , Tokyo, Japan .
FAU - Endo, Kentaro
AU  - Endo K
AD  - Laboratory of Veterinary Surgery, Graduate School of Agriculture and Life 
      Sciences, The University of Tokyo , Tokyo, Japan .
FAU - Morita, Maresuke
AU  - Morita M
AD  - Laboratory of Veterinary Surgery, Graduate School of Agriculture and Life 
      Sciences, The University of Tokyo , Tokyo, Japan .
FAU - Takeda, Tae
AU  - Takeda T
AD  - Laboratory of Veterinary Surgery, Graduate School of Agriculture and Life 
      Sciences, The University of Tokyo , Tokyo, Japan .
FAU - Nakagawa, Takayuki
AU  - Nakagawa T
AD  - Laboratory of Veterinary Surgery, Graduate School of Agriculture and Life 
      Sciences, The University of Tokyo , Tokyo, Japan .
FAU - Nishimura, Ryohei
AU  - Nishimura R
AD  - Laboratory of Veterinary Surgery, Graduate School of Agriculture and Life 
      Sciences, The University of Tokyo , Tokyo, Japan .
LA  - eng
PT  - Journal Article
DEP - 20161203
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adipocytes/*cytology
MH  - Animals
MH  - Antigens, Surface/metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Cell Separation/*methods
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay
MH  - Dogs
MH  - Female
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Multipotent Stem Cells/*cytology
MH  - Time-Lapse Imaging
OTO - NOTNLM
OT  - BM-PAC
OT  - BMMSC
OT  - adipocyte
OT  - bone marrow
OT  - ceiling culture
EDAT- 2016/12/13 06:00
MHDA- 2017/09/19 06:00
CRDT- 2016/12/13 06:00
PHST- 2016/12/13 06:00 [pubmed]
PHST- 2017/09/19 06:00 [medline]
PHST- 2016/12/13 06:00 [entrez]
AID - 10.1089/scd.2016.0200 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2017 Mar 15;26(6):431-440. doi: 10.1089/scd.2016.0200. Epub 2016 
      Dec 3.