PMID- 27938380
OWN - NLM
STAT- MEDLINE
DCOM- 20170929
LR  - 20181113
IS  - 1756-3305 (Electronic)
IS  - 1756-3305 (Linking)
VI  - 9
IP  - 1
DP  - 2016 Dec 9
TI  - Effect of the saliva from different triatomine species on the biology and 
      immunity of TLR-4 ligand and Trypanosoma cruzi-stimulated dendritic cells.
PG  - 634
LID - 634
AB  - BACKGROUND: Triatomines are blood-sucking vectors of Trypanosoma cruzi, the 
      causative agent of Chagas disease. During feeding, triatomines surpass the skin 
      host response through biomolecules present in their saliva. Dendritic cells (DCs) 
      play a crucial role in the induction of the protection to aggressive agents, 
      including blood-sucking arthropods. Here, we evaluated if salivary components of 
      triatomines from different genera evade the host immunity by modulating the 
      biology and the function of LPS- or T. cruzi-stimulated DCs. METHODS: Saliva of 
      Panstrongylus lignarius, Meccus pallidipennis, Triatoma lecticularia and Rhodnius 
      prolixus were obtained by dissection of salivary glands and the DCs were obtained 
      from the differentiation of mouse bone marrow precursors. RESULTS: The 
      differentiation of DCs was inhibited by saliva of all species tested. Saliva 
      differentially inhibited the expression of MHC-II, CD40, CD80 and CD86 in 
      LPS-matured DCs. Except for the saliva of R. prolixus, which induced IL-6 
      cytokine production, TNF-alpha, IL-12 and IL-6 were inhibited by the saliva of the 
      other three tested species and IL-10 was increased in all of them. Saliva per se, 
      also induced the production of IL-12, IL-6 and IL-10. Only the saliva of R. 
      prolixus induced DCs apoptosis. The presence of PGE(2) was not detected in the 
      saliva of the four triatomines studied. Finally, T. cruzi invasion on DCs is 
      enhanced by the presence of the triatomine saliva. CONCLUSIONS: These results 
      demonstrate that saliva from different triatomine species exhibit 
      immunomodulatory effects on LPS and T. cruzi-stimulated DCs. These effects could 
      be related to hematophagy and transmission of T. cruzi during feeding.
FAU - Mendes, Maria Tays
AU  - Mendes MT
AD  - Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, 
      USA.
AD  - Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, Minas 
      Gerais, Brazil.
FAU - Carvalho-Costa, Tamires Marielem
AU  - Carvalho-Costa TM
AD  - Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, Minas 
      Gerais, Brazil.
FAU - da Silva, Marcos Vinicius
AU  - da Silva MV
AD  - Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, Minas 
      Gerais, Brazil. marcosuftm@gmail.com.
FAU - Anhe, Ana Carolina Borella Marfil
AU  - Anhe AC
AD  - Undergraduate Course of Environmental Engineering, Federal University of 
      Triangulo Mineiro, Uberaba, Minas Gerais, Brazil.
FAU - Guimaraes, Rafaela Mano
AU  - Guimaraes RM
AD  - Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, Minas 
      Gerais, Brazil.
FAU - da Costa, Thiago Alvares
AU  - da Costa TA
AD  - Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, Minas 
      Gerais, Brazil.
FAU - Ramirez, Luis Eduardo
AU  - Ramirez LE
AD  - Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, Minas 
      Gerais, Brazil.
FAU - Rodrigues, Virmondes
AU  - Rodrigues V
AD  - Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, Minas 
      Gerais, Brazil.
FAU - Oliveira, Carlo Jose Freire
AU  - Oliveira CJ
AD  - Laboratory of Immunology, Federal University of Triangulo Mineiro, Uberaba, Minas 
      Gerais, Brazil. carlo@icbn.uftm.edu.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161209
PL  - England
TA  - Parasit Vectors
JT  - Parasites & vectors
JID - 101462774
RN  - 0 (Antigens, Surface)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/analysis
MH  - Cell Differentiation/drug effects
MH  - Dendritic Cells/drug effects/*immunology
MH  - Gene Expression
MH  - *Immune Evasion
MH  - *Immune Tolerance
MH  - Mice, Inbred C57BL
MH  - Saliva/*metabolism
MH  - Toll-Like Receptor 4/*metabolism
MH  - Triatominae/*immunology
MH  - Trypanosoma cruzi/*immunology
PMC - PMC5148907
OTO - NOTNLM
OT  - Dendritic cells
OT  - Immunomodulation
OT  - Saliva
OT  - Triatomines
OT  - Trypanosoma cruzi
EDAT- 2016/12/13 06:00
MHDA- 2017/09/30 06:00
PMCR- 2016/12/09
CRDT- 2016/12/13 06:00
PHST- 2016/06/13 00:00 [received]
PHST- 2016/11/16 00:00 [accepted]
PHST- 2016/12/13 06:00 [entrez]
PHST- 2016/12/13 06:00 [pubmed]
PHST- 2017/09/30 06:00 [medline]
PHST- 2016/12/09 00:00 [pmc-release]
AID - 10.1186/s13071-016-1890-x [pii]
AID - 1890 [pii]
AID - 10.1186/s13071-016-1890-x [doi]
PST - epublish
SO  - Parasit Vectors. 2016 Dec 9;9(1):634. doi: 10.1186/s13071-016-1890-x.