PMID- 27939510
OWN - NLM
STAT- MEDLINE
DCOM- 20170410
LR  - 20231213
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 96
DP  - 2017 Mar
TI  - Purification, antitumor and immunomodulatory activity of polysaccharides from 
      soybean residue fermented with Morchella esculenta.
PG  - 26-34
LID - S0141-8130(16)31342-3 [pii]
LID - 10.1016/j.ijbiomac.2016.12.007 [doi]
AB  - Crude polysaccharides (MPS) from soybean residue fermented with Morchella 
      esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and 
      Sephadex G-100 size-exclusion chromatography in sequence. Three main fractions 
      MP-1, MP-3 and MP-4 were obtained during the purification steps. The recovery 
      rates based on MPS used were 26.2%, 29.1% and 18.7% for MP-1, MP-3 and MP-4 
      respectively. The monosaccharide composition, ultraviolet spectrum, infrared 
      spectrum and NMR of the three fractions were analyzed. Furthermore, the influence 
      of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), 
      antitumor activities of the human hepatocellular cell line (HepG-2) and human 
      cervical carcinoma cells (Hela) in vitro were evaluated. The results indicated 
      that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25mug/mL, which is high 
      while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at 
      concentration of 50mug/mL. The fractions of MP-1, MP-3 and MP-4 induced apoptosis 
      in HepG-2 cells and Hela cells by arresting cell cycle progression at the 
      G(0)/G(1) phase. These findings suggest that the purified polysaccharides 
      fractions may be a potent candidate for human hepatocellular and cervical 
      carcinoma treatment and prevention in functional foods and pharmacological 
      fields.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Li, Shuhong
AU  - Li S
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China.
FAU - Gao, Ang
AU  - Gao A
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China.
FAU - Dong, Shuang
AU  - Dong S
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China.
FAU - Chen, Ye
AU  - Chen Y
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China. Electronic address: lshvip@163.com.
FAU - Sun, Shuang
AU  - Sun S
AD  - Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1, 
      Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
FAU - Lei, Zhongfang
AU  - Lei Z
AD  - Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1, 
      Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
FAU - Zhang, Zhenya
AU  - Zhang Z
AD  - Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1, 
      Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.
LA  - eng
PT  - Journal Article
DEP - 20161208
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Immunologic Factors)
RN  - 0 (Monosaccharides)
RN  - 0 (Polysaccharides)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/chemistry/isolation & purification/pharmacology
MH  - Ascomycota/*metabolism
MH  - Cell Cycle/drug effects
MH  - Cell Proliferation/drug effects
MH  - *Fermentation
MH  - HeLa Cells
MH  - Hep G2 Cells
MH  - Humans
MH  - Immunologic Factors/chemistry/isolation & purification/pharmacology
MH  - Macrophages/cytology/drug effects/immunology/metabolism
MH  - Mice
MH  - Monosaccharides/analysis
MH  - Nitric Oxide/biosynthesis
MH  - Phagocytosis/drug effects
MH  - Polysaccharides/chemistry/*isolation & purification/*pharmacology
MH  - RAW 264.7 Cells
MH  - Glycine max/*chemistry/metabolism/*microbiology
OTO - NOTNLM
OT  - Antitumor
OT  - Immunomodulatory
OT  - Polysaccharides
OT  - Purification
OT  - Soybean residue
EDAT- 2016/12/13 06:00
MHDA- 2017/04/11 06:00
CRDT- 2016/12/13 06:00
PHST- 2016/08/24 00:00 [received]
PHST- 2016/11/23 00:00 [revised]
PHST- 2016/12/03 00:00 [accepted]
PHST- 2016/12/13 06:00 [pubmed]
PHST- 2017/04/11 06:00 [medline]
PHST- 2016/12/13 06:00 [entrez]
AID - S0141-8130(16)31342-3 [pii]
AID - 10.1016/j.ijbiomac.2016.12.007 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2017 Mar;96:26-34. doi: 10.1016/j.ijbiomac.2016.12.007. Epub 
      2016 Dec 8.