PMID- 27940202
OWN - NLM
STAT- MEDLINE
DCOM- 20180227
LR  - 20191210
IS  - 1095-953X (Electronic)
IS  - 0969-9961 (Linking)
VI  - 98
DP  - 2017 Feb
TI  - The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell 
      differentiation.
PG  - 137-148
LID - S0969-9961(16)30285-6 [pii]
LID - 10.1016/j.nbd.2016.12.004 [doi]
AB  - Oligodendrocyte and myelin deficits have been reported in mental/psychiatric 
      diseases. The p21-activated kinase 3 (PAK3), a serine/threonine kinase, whose 
      activity is stimulated by the binding of active Rac and Cdc42 GTPases is affected 
      in these pathologies. Indeed, many mutations of Pak3 gene have been described in 
      non-syndromic intellectual disability diseases. Pak3 is expressed mainly in the 
      brain where its role has been investigated in neurons but not in glial cells. 
      Here, we showed that PAK3 is highly expressed in oligodendrocyte precursors 
      (OPCs) and its expression decreases in mature oligodendrocytes. In the developing 
      white matter of the Pak3 knockout mice, we found defects of oligodendrocyte 
      differentiation in the corpus callosum and to a lesser extent in the anterior 
      commissure, which were compensated at the adult stage. In vitro experiments in 
      OPC cultures, derived from Pak3 knockout and wild type brains, support a 
      developmental and cell-autonomous role for PAK3 in regulating OPC differentiation 
      into mature oligodendrocytes. Moreover, we did not detect any obvious alterations 
      of the proliferation or migration of Pak3 null OPCs compared to wild type. 
      Overall, our data highlight PAK3 as a new regulator of OPC differentiation.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Maglorius Renkilaraj, Majistor Raj Luxman
AU  - Maglorius Renkilaraj MRL
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM, CNRS, Institut de Biologie 
      Paris Seine, Neuroscience Paris Seine, F-75005 Paris, France.
FAU - Baudouin, Lucas
AU  - Baudouin L
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM U 1127, CNRS UMR 7225, Institut 
      du Cerveau et de la Moelle epiniere, F-75013 Paris, France.
FAU - Wells, Claire M
AU  - Wells CM
AD  - Division of Cancer Studies, King's College London, UK.
FAU - Doulazmi, Mohamed
AU  - Doulazmi M
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM, CNRS, Institut de Biologie 
      Paris Seine, Adaptation Biologique et vieillissement, F-75005 Paris, France.
FAU - Wehrle, Rosine
AU  - Wehrle R
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM, CNRS, Institut de Biologie 
      Paris Seine, Neuroscience Paris Seine, F-75005 Paris, France.
FAU - Cannaya, Vidjeacoumary
AU  - Cannaya V
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM, CNRS, Institut de Biologie 
      Paris Seine, Neuroscience Paris Seine, F-75005 Paris, France.
FAU - Bachelin, Corinne
AU  - Bachelin C
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM U 1127, CNRS UMR 7225, Institut 
      du Cerveau et de la Moelle epiniere, F-75013 Paris, France.
FAU - Barnier, Jean-Vianney
AU  - Barnier JV
AD  - Institute of Neuroscience Paris-Saclay, CNRS-Universite Paris-Sud, UMR9197, 
      F-91405 Orsay, France.
FAU - Jia, Zhengping
AU  - Jia Z
AD  - Neurosciences & Mental Health, The Hospital for Sick Children, and Department of 
      Physiology, Faculty of Medicine, University of Toronto, 555 University, Toronto, 
      Ontario M5G 1X8, Canada.
FAU - Nait Oumesmar, Brahim
AU  - Nait Oumesmar B
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM U 1127, CNRS UMR 7225, Institut 
      du Cerveau et de la Moelle epiniere, F-75013 Paris, France.
FAU - Dusart, Isabelle
AU  - Dusart I
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM, CNRS, Institut de Biologie 
      Paris Seine, Neuroscience Paris Seine, F-75005 Paris, France.
FAU - Bouslama-Oueghlani, Lamia
AU  - Bouslama-Oueghlani L
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM, CNRS, Institut de Biologie 
      Paris Seine, Neuroscience Paris Seine, F-75005 Paris, France; Sorbonne 
      Universites, UPMC Univ Paris 06, INSERM U 1127, CNRS UMR 7225, Institut du 
      Cerveau et de la Moelle epiniere, F-75013 Paris, France. Electronic address: 
      lamia.bouslama@upmc.fr.
LA  - eng
PT  - Journal Article
DEP - 20161206
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - EC 2.7.11.1 (Pak3 protein, mouse)
RN  - EC 2.7.11.1 (p21-Activated Kinases)
SB  - IM
MH  - Animals
MH  - Anterior Commissure, Brain/cytology/growth & development/metabolism
MH  - Cell Differentiation/*physiology
MH  - Cell Movement/physiology
MH  - Cells, Cultured
MH  - Corpus Callosum/cytology/growth & development/metabolism
MH  - Male
MH  - Mice, Knockout
MH  - Neural Stem Cells/cytology/*metabolism
MH  - Oligodendroglia/cytology/*metabolism
MH  - White Matter/cytology/growth & development/metabolism
MH  - p21-Activated Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - Development
OT  - Differentiation
OT  - Intellectual disability
OT  - Myelin
OT  - OPC
OT  - Oligodendrocyte
OT  - PAK3
EDAT- 2016/12/13 06:00
MHDA- 2018/02/28 06:00
CRDT- 2016/12/13 06:00
PHST- 2016/04/26 00:00 [received]
PHST- 2016/11/04 00:00 [revised]
PHST- 2016/12/02 00:00 [accepted]
PHST- 2016/12/13 06:00 [pubmed]
PHST- 2018/02/28 06:00 [medline]
PHST- 2016/12/13 06:00 [entrez]
AID - S0969-9961(16)30285-6 [pii]
AID - 10.1016/j.nbd.2016.12.004 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2017 Feb;98:137-148. doi: 10.1016/j.nbd.2016.12.004. Epub 2016 Dec 
      6.