PMID- 27942989
OWN - NLM
STAT- MEDLINE
DCOM- 20180216
LR  - 20220410
IS  - 1435-604X (Electronic)
IS  - 0268-8921 (Print)
IS  - 0268-8921 (Linking)
VI  - 32
IP  - 9
DP  - 2017 Dec
TI  - Efficacy of femtosecond lasers for application of acupuncture therapy.
PG  - 2167-2171
LID - 10.1007/s10103-016-2124-3 [doi]
AB  - Acupuncture treatment utilizes the stimulation of metal acupuncture needles that 
      are manually inserted into a living body. In the last decades, laser light has 
      been used as an alternative to needles to stimulate acupuncture points. We 
      previously reported suppression of myostatin (Mstn) gene expression in skeletal 
      muscle by means of femtosecond laser (FL) irradiation, after electroacupuncture, 
      in which acupuncture needles are stimulated with a low-frequency microcurrent. 
      The purpose of the study here was to investigate the efficacy of FL irradiation 
      in mouse skeletal muscle with regard to protein synthesis. After irradiation of 
      the hindlimbs, we first analyzed Mstn gene expression and Mstn protein level in 
      the skeletal muscle. We then evaluated phosphorylation of the mammalian target of 
      rapamycin (mTOR) and its downstream target 70-kDa ribosomal protein S6 kinase 
      (p70S6K). The results showed that FL irradiation significantly reduced the amount 
      of Mstn protein and enhanced the phosphorylation of p70S6K in of the mTOR/S6K 
      signaling pathway. We suggest that FL irradiation activated the protein synthetic 
      pathway in the skeletal muscle. In conclusion, we determined that FL irradiation 
      can serve as an alternative for acupuncture needles and has the potential of 
      being a new non-invasive acupuncture treatment of skeletal muscle.
FAU - Ohta, Mika
AU  - Ohta M
AD  - Division of Medical Informatics and Bioinformatics, Kobe University Hospital, 
      Kobe, 650-0017, Japan.
AD  - Genome Science Research Unit, Life Science Research Center, Kobe Tokiwa 
      University, Kobe, 653-0838, Japan.
FAU - Hosokawa, Yoichiroh
AU  - Hosokawa Y
AD  - Graduate School of Materials Science, Nara Institute of Science and Technology, 
      Nara, 630-0912, Japan.
FAU - Hatano, Naoya
AU  - Hatano N
AD  - The Integrated Center for Mass Spectrometry, Kobe University Graduate School of 
      Medicine, Kobe, 650-0017, Japan.
FAU - Sugano, Aki
AU  - Sugano A
AD  - Division of Medical Informatics and Bioinformatics, Kobe University Hospital, 
      Kobe, 650-0017, Japan.
AD  - Genome Science Research Unit, Life Science Research Center, Kobe Tokiwa 
      University, Kobe, 653-0838, Japan.
FAU - Ito, Akihiko
AU  - Ito A
AD  - Department of Pathology, Kindai University Faculty of Medicine, Osaka, 589-8511, 
      Japan.
FAU - Takaoka, Yutaka
AU  - Takaoka Y
AD  - Division of Medical Informatics and Bioinformatics, Kobe University Hospital, 
      Kobe, 650-0017, Japan. ytakaoka@med.kobe-u.ac.jp.
AD  - Genome Science Research Unit, Life Science Research Center, Kobe Tokiwa 
      University, Kobe, 653-0838, Japan. ytakaoka@med.kobe-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20161210
PL  - England
TA  - Lasers Med Sci
JT  - Lasers in medical science
JID - 8611515
RN  - 0 (Mstn protein, mouse)
RN  - 0 (Myostatin)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (ribosomal protein S6 kinase, 70kD, polypeptide 2)
RN  - EC 2.7.3.2 (Creatine Kinase)
SB  - IM
MH  - Acupuncture Therapy/*methods
MH  - Animals
MH  - Creatine Kinase/blood
MH  - Gene Expression Regulation
MH  - *Lasers
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/metabolism
MH  - Myostatin/genetics/metabolism
MH  - Phosphorylation
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Treatment Outcome
PMC - PMC5682856
OTO - NOTNLM
OT  - Acupuncture therapy
OT  - Femtosecond laser
OT  - Myostatin
OT  - Skeletal muscle
OT  - mTOR
OT  - p70S6K
COIS- CONFLICT OF INTEREST: The authors declare that they have no conflict of interest. 
      FUNDING: This work was partly supported by the Japan Society for the Promotion of 
      Science KAKENHI (Grant Numbers 24590884 and 15K08916, both for M.O.) and a 25th 
      Grant-in-Aid from the Nakatomi Foundation (M.O.). ETHICAL APPROVAL: This study 
      was approved by the Committee for Safe Handling of Living Modified Organisms at 
      Nara Institute of Science and Technology (Permission number 1132) and was carried 
      out according to the guidelines of the committee.
EDAT- 2016/12/13 06:00
MHDA- 2018/02/17 06:00
PMCR- 2016/12/10
CRDT- 2016/12/13 06:00
PHST- 2016/05/29 00:00 [received]
PHST- 2016/11/30 00:00 [accepted]
PHST- 2016/12/13 06:00 [pubmed]
PHST- 2018/02/17 06:00 [medline]
PHST- 2016/12/13 06:00 [entrez]
PHST- 2016/12/10 00:00 [pmc-release]
AID - 10.1007/s10103-016-2124-3 [pii]
AID - 2124 [pii]
AID - 10.1007/s10103-016-2124-3 [doi]
PST - ppublish
SO  - Lasers Med Sci. 2017 Dec;32(9):2167-2171. doi: 10.1007/s10103-016-2124-3. Epub 
      2016 Dec 10.