PMID- 27943061
OWN - NLM
STAT- MEDLINE
DCOM- 20170413
LR  - 20181113
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 53
IP  - 2
DP  - 2017 Apr
TI  - Identification and characterization of host cell proteins interacting with Scylla 
      serrata reovirus non-structural protein p35.
PG  - 317-322
LID - 10.1007/s11262-016-1418-7 [doi]
AB  - We have previously shown that non-structural protein p35, encoded by Scylla 
      serrata reovirus (SsRV) S10, may act as a viroporin. To characterize the role of 
      p35 protein in the modulation of cellular function, a yeast two-hybrid system was 
      used to screen a cDNA library derived from S. serrata to find its interacting 
      partner. Protein interactions were confirmed in vitro by GST pull-down. Full 
      cDNAs of p35 interactors were cloned by the rapid amplification of cDNA ends. 
      After two-hybrid library screening, we isolated partial cDNAs encoding 
      hemocyanin, cryptocyanin, and TAX1BP1. Interaction of p35 with each of 
      hemocyanin, cryptocyanin, and TAX1BP1 was confirmed by GST pull-down. The 
      full-length cDNA fragments of hemocyanin, cryptocyanin, and TAX1BP1 were 2287, 
      2422, and 3437 bp, respectively, and they encoded three putative proteins with 
      molecular masses of ~76.9, ~79.2, and ~107.2 kDa, respectively. This study casts 
      new light on the function and physiological significance of p35 during the SsRV 
      replication cycle.
FAU - Yuan, Yangyang
AU  - Yuan Y
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of 
      China.
FAU - Fan, Dongyang
AU  - Fan D
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of 
      China.
FAU - Zhu, Sidong
AU  - Zhu S
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of 
      China.
FAU - Yang, Jifang
AU  - Yang J
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of 
      China.
FAU - Chen, Jigang
AU  - Chen J
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of 
      China. genomic@163.com.
LA  - eng
PT  - Journal Article
DEP - 20161209
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (DNA, Complementary)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brachyura/*virology
MH  - DNA, Complementary/genetics
MH  - Host-Pathogen Interactions/*genetics
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Reoviridae/*genetics/pathogenicity
MH  - Viral Nonstructural Proteins/*genetics/metabolism
OTO - NOTNLM
OT  - Cryptocyanin
OT  - Hemocyanin
OT  - Scylla serrata reovirus (SsRV)
OT  - TAX1BP1
OT  - Yeast two-hybrid system
EDAT- 2016/12/13 06:00
MHDA- 2017/04/14 06:00
CRDT- 2016/12/13 06:00
PHST- 2016/11/03 00:00 [received]
PHST- 2016/12/02 00:00 [accepted]
PHST- 2016/12/13 06:00 [pubmed]
PHST- 2017/04/14 06:00 [medline]
PHST- 2016/12/13 06:00 [entrez]
AID - 10.1007/s11262-016-1418-7 [pii]
AID - 10.1007/s11262-016-1418-7 [doi]
PST - ppublish
SO  - Virus Genes. 2017 Apr;53(2):317-322. doi: 10.1007/s11262-016-1418-7. Epub 2016 
      Dec 9.