PMID- 27951472
OWN - NLM
STAT- MEDLINE
DCOM- 20171229
LR  - 20180709
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 40
DP  - 2017 Feb
TI  - Soy protein concentrate mitigates markers of colonic inflammation and loss of gut 
      barrier function in vitro and in vivo.
PG  - 201-208
LID - S0955-2863(16)30753-7 [pii]
LID - 10.1016/j.jnutbio.2016.11.012 [doi]
AB  - Whereas a number of studies have examined the effects of soy isoflavones and 
      tocopherols on colonic inflammation, few have examined soy protein. We determined 
      the radical scavenging and cytoprotective effects of soy protein concentrate 
      (SPC) in vitro and its anti-inflammatory effects in dextran sulfate sodium 
      (DSS)-treated mice. Cotreatment with SPC protected Caco-2 human colon cells from 
      H(2)O(2)-induced cell death and mitigated intracellular oxidative stress. 
      Treatment of differentiated Caco-2 cells with SPC blunted DSS-induced increases 
      in monolayer permeability. Pepsin/pancreatin-digested SPC had reduced radical 
      scavenging activity, but retained the monolayer protective effects of SPC. In 
      vivo, 1.5% DSS caused body weight loss, colon shortening, and splenomegaly in 
      CF-1 mice. Co-treatment with 12% SPC mitigated DSS-induced body weight loss and 
      splenomegaly. DSS increased colonic interleukin (IL)-1beta, IL-6, and monocyte 
      chemotactic protein-1 expression. The levels of these markers were significantly 
      lower in mice co-treated with SPC. SPC prevented DSS-mediated reductions in 
      colonic glucagon-like peptide 2 levels, suggesting that SPC can prevent loss of 
      gut barrier function, but no significant effect on claudin 1 and occludin mRNA 
      levels of was observed. SPC-treated mice had lower colonic mRNA expression of 
      toll-like receptor 4 and nucleotide-binding oligomerization domain-containing 
      protein-like receptor family, pyrin domain containing protein 3 (NLRP3), and 
      lower caspase-1 enzyme activity than DSS-treated mice. In summary, SPC exerted 
      antioxidant and cytoprotective effects in vitro and moderated the severity of 
      DSS-induced inflammation and loss of gut barrier function in vivo. These effects 
      appear to be mediated in part through reduced NLRP3 expression and caspase 1 
      activity.
CI  - Copyright (c) 2016. Published by Elsevier Inc.
FAU - Bitzer, Zachary T
AU  - Bitzer ZT
AD  - Department of Food Science, The Pennsylvania State University, University Park, 
      PA, 16802, USA.
FAU - Wopperer, Amy L
AU  - Wopperer AL
AD  - Department of Food Science, The Pennsylvania State University, University Park, 
      PA, 16802, USA.
FAU - Chrisfield, Benjamin J
AU  - Chrisfield BJ
AD  - Department of Food Science, The Pennsylvania State University, University Park, 
      PA, 16802, USA.
FAU - Tao, Ling
AU  - Tao L
AD  - Department of Food Science, The Pennsylvania State University, University Park, 
      PA, 16802, USA.
FAU - Cooper, Timothy K
AU  - Cooper TK
AD  - Department of Comparative Medicine and Department of Pathology, College of 
      Medicine, The Pennsylvania State University, Hershey, PA, 17033, USA.
FAU - Vanamala, Jairam
AU  - Vanamala J
AD  - Department of Food Science, The Pennsylvania State University, University Park, 
      PA, 16802, USA.
FAU - Elias, Ryan J
AU  - Elias RJ
AD  - Department of Food Science, The Pennsylvania State University, University Park, 
      PA, 16802, USA.
FAU - Hayes, John E
AU  - Hayes JE
AD  - Department of Food Science, The Pennsylvania State University, University Park, 
      PA, 16802, USA.
FAU - Lambert, Joshua D
AU  - Lambert JD
AD  - Department of Food Science, The Pennsylvania State University, University Park, 
      PA, 16802, USA; Center for Molecular Toxicology and Carcinogenesis, The 
      Pennsylvania State University, University Park, PA 16802, USA. Electronic 
      address: jdl134@psu.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20161127
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Antioxidants)
RN  - 0 (Biomarkers)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glucagon-Like Peptide 2)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Soybean Proteins)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Biomarkers/metabolism
MH  - Caco-2 Cells
MH  - Chemokine CCL2/metabolism
MH  - Colitis/chemically induced/*drug therapy/*physiopathology
MH  - Dextran Sulfate/toxicity
MH  - Glucagon-Like Peptide 2/metabolism
MH  - Humans
MH  - Inflammasomes/drug effects
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Mice, Inbred Strains
MH  - Permeability
MH  - Soybean Proteins/chemistry/*pharmacology
OTO - NOTNLM
OT  - Colitis
OT  - Dextran sulfate sodium
OT  - Gastrointestinal permeability
OT  - Glycine max
OT  - Inflammasome
OT  - Soy protein
EDAT- 2016/12/13 06:00
MHDA- 2017/12/30 06:00
CRDT- 2016/12/13 06:00
PHST- 2015/04/13 00:00 [received]
PHST- 2016/11/18 00:00 [revised]
PHST- 2016/11/23 00:00 [accepted]
PHST- 2016/12/13 06:00 [pubmed]
PHST- 2017/12/30 06:00 [medline]
PHST- 2016/12/13 06:00 [entrez]
AID - S0955-2863(16)30753-7 [pii]
AID - 10.1016/j.jnutbio.2016.11.012 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2017 Feb;40:201-208. doi: 10.1016/j.jnutbio.2016.11.012. Epub 
      2016 Nov 27.