PMID- 27956627
OWN - NLM
STAT- MEDLINE
DCOM- 20180413
LR  - 20190109
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 113
IP  - 52
DP  - 2016 Dec 27
TI  - Histone deacetylases control module-specific phenotypic plasticity in beetle 
      weapons.
PG  - 15042-15047
LID - 10.1073/pnas.1615688114 [doi]
AB  - Nutritional conditions during early development influence the plastic expression 
      of adult phenotypes. Among several body modules of animals, the development of 
      sexually selected exaggerated traits exhibits striking nutrition sensitivity, 
      resulting in positive allometry and hypervariability distinct from other traits. 
      Using de novo RNA sequencing and comprehensive RNA interference (RNAi) for 
      epigenetic modifying factors, we found that histone deacetylases (HDACs) and 
      polycomb group (PcG) proteins preferentially influence the size of mandibles 
      (exaggerated male weapon) and demonstrate nutrition-dependent hypervariability in 
      the broad-horned flour beetle, Gnatocerus cornutus RNAi-mediated HDAC1 knockdown 
      (KD) in G. cornutus larvae caused specific curtailment of mandibles in adults, 
      whereas HDAC3 KD led to hypertrophy. Notably, these KDs conferred opposite 
      effects on wing size, but little effect on the size of the core body and genital 
      modules. PcG RNAi also reduced adult mandible size. These results suggest that 
      the plastic development of exaggerated traits is controlled in a module-specific 
      manner by HDACs.
FAU - Ozawa, Takane
AU  - Ozawa T
AD  - Department of Life Sciences, Graduate School of Arts and Sciences, The University 
      of Tokyo, Meguro, Tokyo 153-8902, Japan.
FAU - Mizuhara, Tomoko
AU  - Mizuhara T
AD  - Department of Life Sciences, Graduate School of Arts and Sciences, The University 
      of Tokyo, Meguro, Tokyo 153-8902, Japan.
FAU - Arata, Masataka
AU  - Arata M
AD  - Department of Biological Sciences, Graduate School of Science, The University of 
      Tokyo, Bunkyo, Tokyo 113-0033, Japan.
FAU - Shimada, Masakazu
AU  - Shimada M
AD  - Department of General Systems Studies, Graduate School of Arts and Sciences, The 
      University of Tokyo, Meguro, Tokyo 153-8902, Japan.
FAU - Niimi, Teruyuki
AU  - Niimi T
AD  - Division of Evolutionary Developmental Biology, National Institute for Basic 
      Biology, Myodaiji, Okazaki 444-8585, Japan.
FAU - Okada, Kensuke
AU  - Okada K
AD  - Laboratory of Evolutionary Ecology, Graduate School of Environmental Science, 
      Okayama University, Okayama, Okayama 700-8530, Japan.
FAU - Okada, Yasukazu
AU  - Okada Y
AD  - Department of General Systems Studies, Graduate School of Arts and Sciences, The 
      University of Tokyo, Meguro, Tokyo 153-8902, Japan; kohta@bio.c.u-tokyo.ac.jp 
      okayasukazu@gmail.com.
FAU - Ohta, Kunihiro
AU  - Ohta K
AD  - Department of Life Sciences, Graduate School of Arts and Sciences, The University 
      of Tokyo, Meguro, Tokyo 153-8902, Japan; kohta@bio.c.u-tokyo.ac.jp 
      okayasukazu@gmail.com.
AD  - Department of Biological Sciences, Graduate School of Science, The University of 
      Tokyo, Bunkyo, Tokyo 113-0033, Japan.
LA  - eng
SI  - GENBANK/LC096257
SI  - GENBANK/LC096261
SI  - GENBANK/LC100104
SI  - GENBANK/LC100109
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161212
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Insect Proteins)
RN  - 63231-63-0 (RNA)
RN  - EC 2.3.2.27 (Polycomb Repressive Complex 1)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - EC 3.5.1.98 (histone deacetylase 3)
SB  - IM
MH  - Animals
MH  - Coleoptera/*anatomy & histology/*enzymology/genetics
MH  - Drosophila melanogaster/metabolism
MH  - Epigenesis, Genetic
MH  - Histone Deacetylases/genetics/*metabolism
MH  - Insect Proteins/genetics/*metabolism
MH  - Larva
MH  - Male
MH  - Mandible/*anatomy & histology
MH  - Phenotype
MH  - Polycomb Repressive Complex 1/metabolism
MH  - RNA/analysis
MH  - RNA Interference
MH  - Sex Characteristics
PMC - PMC5206575
OTO - NOTNLM
OT  - Gnatocerus cornutus
OT  - HDAC
OT  - exaggerated male weapon
OT  - phenotypic plasticity
OT  - sexual dimorphism
COIS- The authors declare no conflict of interest.
EDAT- 2016/12/14 06:00
MHDA- 2018/04/14 06:00
PMCR- 2016/12/12
CRDT- 2016/12/14 06:00
PHST- 2016/12/14 06:00 [pubmed]
PHST- 2018/04/14 06:00 [medline]
PHST- 2016/12/14 06:00 [entrez]
PHST- 2016/12/12 00:00 [pmc-release]
AID - 1615688114 [pii]
AID - 201615688 [pii]
AID - 10.1073/pnas.1615688114 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2016 Dec 27;113(52):15042-15047. doi: 
      10.1073/pnas.1615688114. Epub 2016 Dec 12.