PMID- 27965080
OWN - NLM
STAT- MEDLINE
DCOM- 20170308
LR  - 20220409
IS  - 1618-0607 (Electronic)
IS  - 1438-4221 (Linking)
VI  - 307
IP  - 2
DP  - 2017 Feb
TI  - Influence of Platelet-rich Plasma on the immune response of human 
      monocyte-derived dendritic cells and macrophages stimulated with Aspergillus 
      fumigatus.
PG  - 95-107
LID - S1438-4221(16)30199-0 [pii]
LID - 10.1016/j.ijmm.2016.11.010 [doi]
AB  - Dendritic cells (DCs) and macrophages (MPhi) are critical for protection against 
      pathogenic fungi including Aspergillus fumigatus. To analyze the role of 
      platelets in the innate immune response, human DCs and MPhis were challenged with 
      A. fumigatus in presence or absence of human platelet rich plasma (PRP). Gene 
      expression analyses and functional investigations were performed. A systems 
      biological approach was used for initial modelling of the DC - A. fumigatus 
      interaction. DCs in a quiescent state together with different corresponding 
      activation states were validated using gene expression data from DCs and MPhi 
      stimulated with A. fumigatus. To characterize the influence of platelets on the 
      immune response of DCs and MPhi to A. fumigatus, we experimentally quantified their 
      cytokine secretion, phagocytic capacity, maturation, and metabolic activity with 
      or without platelets. PRP in combination with A. fumigatus treatment resulted in 
      the highest expression of the maturation markers CD80, CD83 and CD86 in DCs. 
      Furthermore, PRP enhanced the capacity of macrophages and DCs to phagocytose A. 
      fumigatus conidia. In parallel, PRP in combination with the innate immune cells 
      significantly reduced the metabolic activity of the fungus. Interestingly, A. 
      fumigatus and PRP stimulated MPhi showed a significantly reduced gene expression 
      and secretion of IL6 while PRP only reduced the IL-6 secretion of A. fumigatus 
      stimulated DCs. The in silico systems biological model correlated well with these 
      experimental data. Different modules centrally involved in DC function became 
      clearly apparent, including DC maturation, cytokine response and apoptosis 
      pathways. Taken together, the ability of PRP to suppress IL-6 release of human 
      DCs might prevent local excessive inflammatory hemorrhage, tissue infarction and 
      necrosis in the human lung.
CI  - Copyright (c) 2016 Elsevier GmbH. All rights reserved.
FAU - Czakai, Kristin
AU  - Czakai K
AD  - Department of Internal Medicine, University Hospital of Wurzburg, Wurzburg, 
      Germany.
FAU - Dittrich, Marcus
AU  - Dittrich M
AD  - Department of Bioinformatics, Biocenter, University of Wurzburg, Am Hubland, 
      Wurzburg, Germany.
FAU - Kaltdorf, Martin
AU  - Kaltdorf M
AD  - Department of Bioinformatics, Biocenter, University of Wurzburg, Am Hubland, 
      Wurzburg, Germany.
FAU - Muller, Tobias
AU  - Muller T
AD  - Department of Bioinformatics, Biocenter, University of Wurzburg, Am Hubland, 
      Wurzburg, Germany.
FAU - Krappmann, Sven
AU  - Krappmann S
AD  - Microbiology Institute-Clinical Microbiology, Immunology and Hygiene, University 
      Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nurnberg, Erlangen, 
      Germany.
FAU - Schedler, Anette
AU  - Schedler A
AD  - Department of Internal Medicine, University Hospital of Wurzburg, Wurzburg, 
      Germany.
FAU - Bonin, Michael
AU  - Bonin M
AD  - IMGM Laboratories GmbH, Planegg, Germany.
FAU - Duhring, Sybille
AU  - Duhring S
AD  - Deparment of Bioinformatics, Friedrich-Schiller-University Jena, Jena, Germany.
FAU - Schuster, Stefan
AU  - Schuster S
AD  - Deparment of Bioinformatics, Friedrich-Schiller-University Jena, Jena, Germany.
FAU - Speth, Cornelia
AU  - Speth C
AD  - Hygiene und Medizinische Mikrobiologie, Medizinische Universitat Innsbruck, 
      Innsbruck, Austria.
FAU - Rambach, Gunter
AU  - Rambach G
AD  - Hygiene und Medizinische Mikrobiologie, Medizinische Universitat Innsbruck, 
      Innsbruck, Austria.
FAU - Einsele, Hermann
AU  - Einsele H
AD  - Department of Internal Medicine, University Hospital of Wurzburg, Wurzburg, 
      Germany.
FAU - Dandekar, Thomas
AU  - Dandekar T
AD  - Department of Bioinformatics, Biocenter, University of Wurzburg, Am Hubland, 
      Wurzburg, Germany.
FAU - Loffler, Jurgen
AU  - Loffler J
AD  - Department of Internal Medicine, University Hospital of Wurzburg, Wurzburg, 
      Germany. Electronic address: loeffler_j@ukw.de.
LA  - eng
PT  - Journal Article
DEP - 20161209
PL  - Germany
TA  - Int J Med Microbiol
JT  - International journal of medical microbiology : IJMM
JID - 100898849
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Aspergillus fumigatus/*immunology
MH  - Cell Differentiation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Gene Expression Profiling
MH  - Healthy Volunteers
MH  - Humans
MH  - Macrophages/*immunology
MH  - Phagocytosis
MH  - Platelet-Rich Plasma/*metabolism
OTO - NOTNLM
OT  - Boolean model
OT  - Gene ontology
OT  - IL-6
OT  - Network
OT  - Platelets
OT  - Transcriptional profiling
EDAT- 2016/12/15 06:00
MHDA- 2017/03/09 06:00
CRDT- 2016/12/15 06:00
PHST- 2016/08/02 00:00 [received]
PHST- 2016/11/22 00:00 [revised]
PHST- 2016/11/27 00:00 [accepted]
PHST- 2016/12/15 06:00 [pubmed]
PHST- 2017/03/09 06:00 [medline]
PHST- 2016/12/15 06:00 [entrez]
AID - S1438-4221(16)30199-0 [pii]
AID - 10.1016/j.ijmm.2016.11.010 [doi]
PST - ppublish
SO  - Int J Med Microbiol. 2017 Feb;307(2):95-107. doi: 10.1016/j.ijmm.2016.11.010. 
      Epub 2016 Dec 9.