PMID- 27973444
OWN - NLM
STAT- MEDLINE
DCOM- 20170403
LR  - 20240326
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 17
IP  - 12
DP  - 2016 Dec 12
TI  - Differential Cytotoxic Potential of Silver Nanoparticles in Human Ovarian Cancer 
      Cells and Ovarian Cancer Stem Cells.
LID - 2077
AB  - The cancer stem cell (CSC) hypothesis postulates that cancer cells are composed 
      of hierarchically-organized subpopulations of cells with distinct phenotypes and 
      tumorigenic capacities. As a result, CSCs have been suggested as a source of 
      disease recurrence. Recently, silver nanoparticles (AgNPs) have been used as 
      antimicrobial, disinfectant, and antitumor agents. However, there is no study 
      reporting the effects of AgNPs on ovarian cancer stem cells (OvCSCs). In this 
      study, we investigated the cytotoxic effects of AgNPs and their mechanism of 
      causing cell death in A2780 (human ovarian cancer cells) and OvCSCs derived from 
      A2780. In order to examine these effects, OvCSCs were isolated and characterized 
      using positive CSC markers including aldehyde dehydrogenase (ALDH) and CD133 by 
      fluorescence-activated cell sorting (FACS). The anticancer properties of the 
      AgNPs were evaluated by assessing cell viability, leakage of lactate 
      dehydrogenase (LDH), reactive oxygen species (ROS), and mitochondrial membrane 
      potential (mt-MP). The inhibitory effect of AgNPs on the growth of ovarian cancer 
      cells and OvCSCs was evaluated using a clonogenic assay. Following 1-2 weeks of 
      incubation with the AgNPs, the numbers of A2780 (bulk cells) and ALDH(+)/CD133(+) 
      colonies were significantly reduced. The expression of apoptotic and 
      anti-apoptotic genes was measured by real-time quantitative reverse transcriptase 
      polymerase chain reaction (qRT-PCR). Our observations showed that treatment with 
      AgNPs resulted in severe cytotoxicity in both ovarian cancer cells and OvCSCs. In 
      particular, AgNPs showed significant cytotoxic potential in ALDH(+)/CD133(+) 
      subpopulations of cells compared with other subpopulation of cells and also human 
      ovarian cancer cells (bulk cells). These findings suggest that AgNPs can be 
      utilized in the development of novel nanotherapeutic molecules for the treatment 
      of ovarian cancers by specific targeting of the ALDH(+)/CD133(+) subpopulation of 
      cells.
FAU - Choi, Yun-Jung
AU  - Choi YJ
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      143-701, Korea. yunjungc@konkuk.ac.kr.
FAU - Park, Jung-Hyun
AU  - Park JH
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      143-701, Korea. saladinhyun@naver.com.
FAU - Han, Jae Woong
AU  - Han JW
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      143-701, Korea. woong1211@naver.com.
FAU - Kim, Eunsu
AU  - Kim E
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      143-701, Korea. np-gennao@hanmail.net.
FAU - Jae-Wook, Oh
AU  - Jae-Wook O
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      143-701, Korea. ohjw62@gmail.com.
FAU - Lee, Seung Yoon
AU  - Lee SY
AD  - Swine Consulting Group, HanByol Farm Tech, Gyeonggi 463-785, Korea. 
      kissleevet@gmail.com.
FAU - Kim, Jin-Hoi
AU  - Kim JH
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      143-701, Korea. jhkim541@konkuk.ac.kr.
FAU - Gurunathan, Sangiliyandi
AU  - Gurunathan S
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      143-701, Korea. sangiliyandi@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20161212
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (AC133 Antigen)
RN  - 3M4G523W1G (Silver)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
SB  - IM
MH  - AC133 Antigen/metabolism
MH  - Aldehyde Dehydrogenase/metabolism
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Cell Separation
MH  - Cell Survival/drug effects
MH  - Female
MH  - Humans
MH  - Metal Nanoparticles/*chemistry
MH  - Neoplastic Stem Cells/drug effects/*pathology
MH  - Ovarian Neoplasms/*pathology
MH  - Silver/*pharmacology
MH  - Time Factors
MH  - Tumor Stem Cell Assay
PMC - PMC5187877
OTO - NOTNLM
OT  - cancer therapy
OT  - cell viability
OT  - cytotoxicity
OT  - ovarian cancer cells
OT  - ovarian cancer stem cells
OT  - silver nanoparticles
COIS- The authors declare no conflict of interest.
EDAT- 2016/12/16 06:00
MHDA- 2017/04/04 06:00
PMCR- 2016/12/01
CRDT- 2016/12/16 06:00
PHST- 2016/09/09 00:00 [received]
PHST- 2016/11/17 00:00 [revised]
PHST- 2016/11/30 00:00 [accepted]
PHST- 2016/12/16 06:00 [entrez]
PHST- 2016/12/16 06:00 [pubmed]
PHST- 2017/04/04 06:00 [medline]
PHST- 2016/12/01 00:00 [pmc-release]
AID - ijms17122077 [pii]
AID - ijms-17-02077 [pii]
AID - 10.3390/ijms17122077 [doi]
PST - epublish
SO  - Int J Mol Sci. 2016 Dec 12;17(12):2077. doi: 10.3390/ijms17122077.