PMID- 27977864
OWN - NLM
STAT- MEDLINE
DCOM- 20170911
LR  - 20181025
IS  - 1097-4598 (Electronic)
IS  - 0148-639X (Linking)
VI  - 56
IP  - 3
DP  - 2017 Sep
TI  - N-methyl-D-aspartate (NMDA) impairs myogenesis in C2C12 cells.
PG  - 510-518
LID - 10.1002/mus.25511 [doi]
AB  - INTRODUCTION: N-methyl-d-aspartate (NMDA) is expressed in sensory neurons and 
      plays important roles in peripheral pain mechanisms. The aim of this study was to 
      examine the effects and molecular mechanisms of NMDA on C2C12 myoblast 
      proliferation and differentiation. METHODS: Cytotoxicity and differentiation were 
      examined by the MTT assay, reverse transcription-polymerase chain reaction, and 
      immunofluorescence. RESULTS: NMDA had no cytotoxicity (10-500 muM) and inhibited 
      myoblastic differentiation of C2C12 cells, as assessed by F-actin 
      immunofluorescence and levels of mRNAs encoding myogenic markers such as myogenin 
      and myosin heavy-chain 2. It inhibited phosphorylation of mammalian target of 
      rapamycin (mTOR) by inactivating mitogen-activated protein kinases (MAPKs), 
      including extracellular signal-regulated kinase, c-Jun N-terminal kinase, and 
      p38. It induced reactive oxygen species production. Furthermore, NMDA-suppressed 
      expression of F-actin was reversed by adding the antioxidant N-acetylcysteine. 
      CONCLUSIONS: Collectively, these results indicate that NMDA impairs myogenesis or 
      myogenic differentiation in C2C12 cells through the mTOR/MAPK signaling pathways 
      and may lead to skeletal muscle degeneration. Muscle Nerve 56: 510-518, 2017.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Auh, Q-SChick
AU  - Auh QS
AD  - Department of Oral Medicine, School of Dentistry, Kyung Hee University, Seoul, 
      Korea.
FAU - Park, Kyung-Ran
AU  - Park KR
AD  - Department of Oral and Maxillofacial Pathology, MRC, School of Dentistry, Kyung 
      Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
FAU - Lee, Myeong-Ok
AU  - Lee MO
AD  - Department of Dentistry, Graduate School, Kyung Hee University, Seoul, Korea.
FAU - Hwang, Mi-Jin
AU  - Hwang MJ
AD  - Department of Dentistry, Graduate School, Kyung Hee University, Seoul, Korea.
FAU - Kang, Soo-Kyung
AU  - Kang SK
AD  - Department of Oral Medicine, School of Dentistry, Kyung Hee University, Seoul, 
      Korea.
FAU - Hong, Jung-Pyo
AU  - Hong JP
AD  - Department of Oral Medicine, School of Dentistry, Kyung Hee University, Seoul, 
      Korea.
FAU - Yun, Hyung-Mun
AU  - Yun HM
AD  - Department of Oral and Maxillofacial Pathology, MRC, School of Dentistry, Kyung 
      Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
FAU - Kim, Eun-Cheol
AU  - Kim EC
AD  - Department of Oral and Maxillofacial Pathology, MRC, School of Dentistry, Kyung 
      Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170310
PL  - United States
TA  - Muscle Nerve
JT  - Muscle & nerve
JID - 7803146
RN  - 0 (Reactive Oxygen Species)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects/physiology
MH  - Cell Line
MH  - Cell Proliferation/*drug effects/physiology
MH  - Mice
MH  - Muscle Development/*drug effects/physiology
MH  - Myoblasts/*drug effects/metabolism
MH  - N-Methylaspartate/*toxicity
MH  - Reactive Oxygen Species/antagonists & inhibitors/metabolism
OTO - NOTNLM
OT  - NMDA
OT  - ROS
OT  - differentiation
OT  - mTOR
OT  - myalgic disorder
OT  - myogenesis
EDAT- 2016/12/16 06:00
MHDA- 2017/09/12 06:00
CRDT- 2016/12/16 06:00
PHST- 2016/08/01 00:00 [received]
PHST- 2016/11/07 00:00 [revised]
PHST- 2016/12/07 00:00 [accepted]
PHST- 2016/12/16 06:00 [pubmed]
PHST- 2017/09/12 06:00 [medline]
PHST- 2016/12/16 06:00 [entrez]
AID - 10.1002/mus.25511 [doi]
PST - ppublish
SO  - Muscle Nerve. 2017 Sep;56(3):510-518. doi: 10.1002/mus.25511. Epub 2017 Mar 10.