PMID- 27979921
OWN - NLM
STAT- MEDLINE
DCOM- 20170703
LR  - 20210109
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 36
IP  - 3
DP  - 2017 Feb 1
TI  - Complex structure of cytochrome c-cytochrome c oxidase reveals a novel 
      protein-protein interaction mode.
PG  - 291-300
LID - 10.15252/embj.201695021 [doi]
AB  - Mitochondrial cytochrome c oxidase (CcO) transfers electrons from cytochrome c 
      (Cyt.c) to O(2) to generate H(2)O, a process coupled to proton pumping. To 
      elucidate the mechanism of electron transfer, we determined the structure of the 
      mammalian Cyt.c-CcO complex at 2.0-A resolution and identified an electron 
      transfer pathway from Cyt.c to CcO. The specific interaction between Cyt.c and 
      CcO is stabilized by a few electrostatic interactions between side chains within 
      a small contact surface area. Between the two proteins are three water layers 
      with a long inter-molecular span, one of which lies between the other two layers 
      without significant direct interaction with either protein. Cyt.c undergoes large 
      structural fluctuations, using the interacting regions with CcO as a fulcrum. 
      These features of the protein-protein interaction at the docking interface 
      represent the first known example of a new class of protein-protein interaction, 
      which we term "soft and specific". This interaction is likely to contribute to 
      the rapid association/dissociation of the Cyt.c-CcO complex, which facilitates 
      the sequential supply of four electrons for the O(2) reduction reaction.
CI  - (c) 2016 The Authors. Published under the terms of the CC BY 4.0 license.
FAU - Shimada, Satoru
AU  - Shimada S
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan.
FAU - Shinzawa-Itoh, Kyoko
AU  - Shinzawa-Itoh K
AUID- ORCID: 0000-0002-4571-2218
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan shinzawa@sci.u-hyogo.ac.jp tsuki@protein.osaka-u.ac.jp.
FAU - Baba, Junpei
AU  - Baba J
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan.
FAU - Aoe, Shimpei
AU  - Aoe S
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan.
FAU - Shimada, Atsuhiro
AU  - Shimada A
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan.
FAU - Yamashita, Eiki
AU  - Yamashita E
AD  - Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
FAU - Kang, Jiyoung
AU  - Kang J
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan.
FAU - Tateno, Masaru
AU  - Tateno M
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan.
FAU - Yoshikawa, Shinya
AU  - Yoshikawa S
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan.
FAU - Tsukihara, Tomitake
AU  - Tsukihara T
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      Akoh, Hyogo, Japan shinzawa@sci.u-hyogo.ac.jp tsuki@protein.osaka-u.ac.jp.
AD  - Institute for Protein Research, Osaka University, Suita, Osaka, Japan.
AD  - JST, CREST, Kawaguchi, Saitama, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161215
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 059QF0KO0R (Water)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - S88TT14065 (Oxygen)
SB  - IM
CIN - EMBO J. 2017 Feb 1;36(3):250-251. PMID: 28087581
MH  - Animals
MH  - Cattle
MH  - Crystallography, X-Ray
MH  - Cytochromes c/*chemistry/*metabolism
MH  - Electron Transport
MH  - Electron Transport Complex IV/*chemistry/*metabolism
MH  - Horses
MH  - Models, Biological
MH  - Models, Molecular
MH  - Oxygen/metabolism
MH  - Protein Binding
MH  - Protein Conformation
MH  - Water/metabolism
PMC - PMC5286356
OTO - NOTNLM
OT  - X-ray crystallography
OT  - cytochrome c
OT  - cytochrome c oxidase
OT  - electron transfer complex
OT  - protein-protein interaction
EDAT- 2016/12/17 06:00
MHDA- 2017/07/04 06:00
PMCR- 2016/12/15
CRDT- 2016/12/17 06:00
PHST- 2016/06/16 00:00 [received]
PHST- 2016/10/21 00:00 [revised]
PHST- 2016/11/16 00:00 [accepted]
PHST- 2016/12/17 06:00 [pubmed]
PHST- 2017/07/04 06:00 [medline]
PHST- 2016/12/17 06:00 [entrez]
PHST- 2016/12/15 00:00 [pmc-release]
AID - embj.201695021 [pii]
AID - EMBJ201695021 [pii]
AID - 10.15252/embj.201695021 [doi]
PST - ppublish
SO  - EMBO J. 2017 Feb 1;36(3):291-300. doi: 10.15252/embj.201695021. Epub 2016 Dec 15.