PMID- 27980586
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1735-0328 (Print)
IS  - 1726-6890 (Electronic)
IS  - 1726-6882 (Linking)
VI  - 15
IP  - 3
DP  - 2016 Summer
TI  - Antiproliferative Activity and Apoptosis Inducing Mechanism of Anthocephalus 
      cadamba on Dalton's Lymphoma Ascites Cells.
PG  - 505-514
AB  - The purpose of this investigation was to evaluate the antiproliferative and 
      apoptogenic mechanistic studies of methanol extract of Anthocephalus cadamba 
      (MEAC) on Dalton's lymphoma ascites (DLA) cells treated mice. Determination of 
      antiproliferative activity was performed by using different DLA cells (2x10(6) 
      cells, i.p.) inoculated mice groups (n = 12). Groups were treated for 14 
      consecutive days with MEAC at the doses of 200 and 400 mg/Kg b.w. respectively. 
      The mechanism of antiproliferation activity of MEAC was investigated through 
      morphological studies by acridine orange (AO)/ethidium bromide (EB) double 
      staining method. Comet assay was estimated to check the DNA damage induced 
      apoptosis property. Furthermore, flow cytometry (FACS) was used to quantitatively 
      detect the apoptotic rate by double labeling techniques using Annexin-V 
      FITC/propidium iodide staining analysis and apoptotic proteins expression done by 
      western blotting assay method. MEAC exhibited significant (p<0.01) decrease the 
      tumor volume, viable cell count, tumor weight and elevated the life span of DLA 
      tumor bearing mice. Analysis of AO/EB staining and flow cytometry showed that 
      MEAC possessed apoptosis induced antitumor activity on DLA cells in a dose 
      dependant manner. Dose dependent induction of DNA damage on DLA cells were 
      observed after MEAC treatment, which was evident from the appearance of comet 
      tail length. Pro-apoptotic gene, Bax was up-regulated and down-regulation of the 
      Bcl-2/Bax ratio, suggesting that Bcl-2 family involved in the control of 
      apoptosis. Experimental results revealed that MEAC possess potent antitumor 
      activity via induction of cancer cell apoptosis mechanism.
FAU - Dolai, Narayan
AU  - Dolai N
AD  - Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
FAU - Islam, Aminul
AU  - Islam A
AD  - Research and Development Centre, Natreon Inc, Salt Lake City, Kolkata, India.
FAU - Haldar, Pallab Kanti
AU  - Haldar PK
AD  - Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Iran J Pharm Res
JT  - Iranian journal of pharmaceutical research : IJPR
JID - 101208407
PMC - PMC5149038
OTO - NOTNLM
OT  - Anthocephalus cadamba
OT  - Antitumor
OT  - Apoptosis
OT  - DLA
EDAT- 2016/12/17 06:00
MHDA- 2016/12/17 06:01
PMCR- 2016/06/01
CRDT- 2016/12/17 06:00
PHST- 2016/12/17 06:00 [entrez]
PHST- 2016/12/17 06:00 [pubmed]
PHST- 2016/12/17 06:01 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - ijpr-15-505 [pii]
PST - ppublish
SO  - Iran J Pharm Res. 2016 Summer;15(3):505-514.