PMID- 27980913
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210109
IS  - 2198-3844 (Print)
IS  - 2198-3844 (Electronic)
IS  - 2198-3844 (Linking)
VI  - 2
IP  - 11
DP  - 2015 Nov
TI  - Polymer-Coated Metal-Oxide Nanoparticles Inhibit IgE Receptor Binding, Cellular 
      Signaling, and Degranulation in a Mast Cell-like Cell Line.
PG  - 1500104
LID - 1500104
AB  - Previous reports have shown that nanoparticles (NPs) can both enhance and 
      suppress immune effector functions; however the mechanisms that dictate these 
      responses are still unclear. Here, the effects of polyacrylic acid (PAA) 
      functionalized metal-oxide NP are investigated on RBL-2H3 (representative 
      mammalian granulocyte-like cell line) cell viability, cellular degranulation, 
      immunoglobulin E (IgE) receptor binding, and cell signaling pathways related to 
      immune function. The increasing development of PAA-NPs as pesticide dispersants 
      and as drug carriers in therapeutics necessitates their investigation for safe 
      production. Using two in vitro experimental approaches, this study demonstrates 
      that pre-exposing RBL-2H3 cells, or IgE antibodies, to PAA-NPs (TiO(2), CeO(2), 
      ZnO, Fe(2)O(3), and PAA-Capsules (NP coating control) over 24 h, significantly 
      decrease the binding capacity of IgE for Fcepsilon receptors, inhibit the 
      phosphorylation of intracellular signaling proteins (e.g., MAPK ERK) that mediate 
      degranulation, and inhibited RBL-2H3 cell degranulation. In addition, and unlike 
      the other NPs tested, PAA-TiO(2) significantly reduced RBL-2H3 viability, in a 
      time (4-24 h) and dose-dependent manner (>50 mug mL(-1)). Together, these data 
      demonstrate that PAA-NPs at sub-lethal doses can interact with cell surface 
      structures, such as receptors, to suppress various stages of the RBL-2H3 
      degranulatory response to external stimuli, and modify immune cell functions that 
      can impact host-immunity.
FAU - Ortega, Van A
AU  - Ortega VA
AD  - Department of Biological Sciences University of Alberta Edmonton Alberta Canada 
      T6G 2E9.
FAU - Ede, James D
AU  - Ede JD
AD  - Department of Biological Sciences University of Alberta Edmonton Alberta Canada 
      T6G 2E9.
FAU - Boyle, David
AU  - Boyle D
AD  - Department of Biological Sciences University of Alberta Edmonton Alberta Canada 
      T6G 2E9.
FAU - Stafford, James L
AU  - Stafford JL
AD  - Department of Biological Sciences University of Alberta Edmonton Alberta Canada 
      T6G 2E9.
FAU - Goss, Greg G
AU  - Goss GG
AD  - Department of Biological Sciences University of Alberta Edmonton Alberta Canada 
      T6G 2E9; National Research Council (Canada)National Institute for Nanotechnology 
      Edmonton Alberta Canada T6G 2M9.
LA  - eng
PT  - Journal Article
DEP - 20150714
PL  - Germany
TA  - Adv Sci (Weinh)
JT  - Advanced science (Weinheim, Baden-Wurttemberg, Germany)
JID - 101664569
PMC - PMC5115347
OTO - NOTNLM
OT  - cytotoxicity
OT  - immunology
OT  - nanoparticles
OT  - receptors
OT  - viability
EDAT- 2015/07/14 00:00
MHDA- 2015/07/14 00:01
PMCR- 2015/07/14
CRDT- 2016/12/17 06:00
PHST- 2015/03/25 00:00 [received]
PHST- 2015/06/07 00:00 [revised]
PHST- 2016/12/17 06:00 [entrez]
PHST- 2015/07/14 00:00 [pubmed]
PHST- 2015/07/14 00:01 [medline]
PHST- 2015/07/14 00:00 [pmc-release]
AID - ADVS201500104 [pii]
AID - 10.1002/advs.201500104 [doi]
PST - epublish
SO  - Adv Sci (Weinh). 2015 Jul 14;2(11):1500104. doi: 10.1002/advs.201500104. 
      eCollection 2015 Nov.