PMID- 27981498
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20191008
IS  - 1559-1182 (Electronic)
IS  - 0893-7648 (Print)
IS  - 0893-7648 (Linking)
VI  - 55
IP  - 1
DP  - 2018 Jan
TI  - Humoral Immunity Profiling of Subjects with Myalgic Encephalomyelitis Using a 
      Random Peptide Microarray Differentiates Cases from Controls with High 
      Specificity and Sensitivity.
PG  - 633-641
LID - 10.1007/s12035-016-0334-0 [doi]
AB  - Myalgic encephalomyelitis (ME) is a complex, heterogeneous illness of unknown 
      etiology. The search for biomarkers that can delineate cases from controls is one 
      of the most active areas of ME research; however, little progress has been made 
      in achieving this goal. In contrast to identifying biomarkers that are directly 
      involved in the pathological process, an immunosignature identifies antibodies 
      raised to proteins expressed during, and potentially involved in, the 
      pathological process. Although these proteins might be unknown, it is possible to 
      detect antibodies that react to these proteins using random peptide arrays. In 
      the present study, we probe a custom 125,000 random 12-mer peptide microarray 
      with sera from 21 ME cases and 21 controls from the USA and Europe and used these 
      data to develop a diagnostic signature. We further used these peptide sequences 
      to potentially uncover the naturally occurring candidate antigens to which these 
      antibodies may specifically react with in vivo. Our analysis revealed a subset of 
      25 peptides that distinguished cases and controls with high specificity and 
      sensitivity. Additionally, Basic Local Alignment Search Tool (BLAST) searches 
      suggest that these peptides primarily represent human self-antigens and 
      endogenous retroviral sequences and, to a minor extent, viral and bacterial 
      pathogens.
FAU - Singh, Sahajpreet
AU  - Singh S
AD  - Nevada Center for Biomedical Research, 1664 N Virginia St. MS 0552, Reno, NV, 
      89557-0552, USA.
FAU - Stafford, Phillip
AU  - Stafford P
AD  - The Biodesign Institute Center for Innovations in Medicine at Arizona State 
      University, Tempe, AZ, USA.
FAU - Schlauch, Karen A
AU  - Schlauch KA
AD  - Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 
      USA.
AD  - Nevada INBRE Bioinformatics Core, University of Nevada, Reno, NV, USA.
FAU - Tillett, Richard R
AU  - Tillett RR
AD  - Nevada INBRE Bioinformatics Core, University of Nevada, Reno, NV, USA.
FAU - Gollery, Martin
AU  - Gollery M
AD  - Tahoe Bioinformatics, Incline Village, Reno, NV, USA.
FAU - Johnston, Stephen Albert
AU  - Johnston SA
AD  - The Biodesign Institute Center for Innovations in Medicine at Arizona State 
      University, Tempe, AZ, USA.
FAU - Khaiboullina, Svetlana F
AU  - Khaiboullina SF
AD  - Nevada Center for Biomedical Research, 1664 N Virginia St. MS 0552, Reno, NV, 
      89557-0552, USA.
AD  - Kazan Federal University, Kazan, Russian Federation.
FAU - De Meirleir, Kenny L
AU  - De Meirleir KL
AD  - Nevada Center for Biomedical Research, 1664 N Virginia St. MS 0552, Reno, NV, 
      89557-0552, USA.
FAU - Rawat, Shanti
AU  - Rawat S
AD  - Nevada Center for Biomedical Research, 1664 N Virginia St. MS 0552, Reno, NV, 
      89557-0552, USA.
FAU - Mijatovic, Tatjana
AU  - Mijatovic T
AD  - R.E.D. Laboratories, Zellik, Belgium.
FAU - Subramanian, Krishnamurthy
AU  - Subramanian K
AD  - Nevada Center for Biomedical Research, 1664 N Virginia St. MS 0552, Reno, NV, 
      89557-0552, USA.
FAU - Palotas, Andras
AU  - Palotas A
AD  - Kazan Federal University, Kazan, Russian Federation. palotas@asklepios-med.eu.
AD  - Asklepios-Med (private medical practice and research center), Kossuth Lajos sgt. 
      23, Szeged, 6722, Hungary. palotas@asklepios-med.eu.
FAU - Lombardi, Vincent C
AU  - Lombardi VC
AD  - Nevada Center for Biomedical Research, 1664 N Virginia St. MS 0552, Reno, NV, 
      89557-0552, USA. vclombardi@nvcbr.org.
AD  - Department of Pharmacology, University of Nevada, Reno, School of Medicine, Reno, 
      NV, USA. vclombardi@nvcbr.org.
LA  - eng
GR  - P20 GM103440/GM/NIGMS NIH HHS/United States
GR  - R01 AI078234/AI/NIAID NIH HHS/United States
GR  - GM103440/National Institute of General Medical Sciences (US)/International
GR  - R01AI078234/NH/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20161215
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
RN  - 0 (Peptides)
SB  - IM
MH  - Algorithms
MH  - Amino Acid Sequence
MH  - Case-Control Studies
MH  - Fatigue Syndrome, Chronic/*immunology
MH  - Humans
MH  - *Immunity, Humoral
MH  - Peptides/chemistry/*metabolism
MH  - *Protein Array Analysis
MH  - Sensitivity and Specificity
MH  - Sequence Alignment
PMC - PMC5472503
MID - NIHMS839962
OTO - NOTNLM
OT  - Antibody
OT  - Chronic fatigue syndrome
OT  - Immunosignature
OT  - Myalgic encephalomyelitis
OT  - Peptide array
EDAT- 2016/12/17 06:00
MHDA- 2018/12/12 06:00
PMCR- 2016/12/15
CRDT- 2016/12/17 06:00
PHST- 2016/06/03 00:00 [received]
PHST- 2016/11/29 00:00 [accepted]
PHST- 2016/12/17 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2016/12/17 06:00 [entrez]
PHST- 2016/12/15 00:00 [pmc-release]
AID - 10.1007/s12035-016-0334-0 [pii]
AID - 334 [pii]
AID - 10.1007/s12035-016-0334-0 [doi]
PST - ppublish
SO  - Mol Neurobiol. 2018 Jan;55(1):633-641. doi: 10.1007/s12035-016-0334-0. Epub 2016 
      Dec 15.