PMID- 27981544
OWN - NLM
STAT- MEDLINE
DCOM- 20170626
LR  - 20181202
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 20
IP  - 23
DP  - 2016 Dec
TI  - Human umbilical cord mesenchymal stem cells ameliorate experimental cirrhosis 
      through activation of keratinocyte growth factor by suppressing microRNA-199.
PG  - 4905-4912
LID - 11855 [pii]
AB  - OBJECTIVE: Keratinocyte growth factor (KGF) has a demonstrated role in the 
      prevention of cirrhosis during liver regeneration. Previous studies have shown 
      that transplantation of human umbilical cord mesenchymal stem cells (HUCMSCs) 
      reduces the development of cirrhosis after liver injury. However, whether KGF may 
      be involved in the underlying molecular mechanisms remains unknown. Here we 
      addressed this question. MATERIALS AND METHODS: We did HUCMSC transplantation in 
      mice that had developed cirrhosis by carbon tetrachloride (CCl4). The effects of 
      UCMSC transplantation on KGF levels and liver damage were examined. The level of 
      a KGF-targeting microRNA, miR-199, was examined. The regulation of KGF by miR-199 
      was studied by bioinformatics analyses and luciferase reporter assay. RESULTS: 
      HUCMSC transplantation significantly ameliorated the severity of liver fibrosis, 
      reduced portal hypertension and sodium retention that were induced by CCl4. 
      HUCMSC transplantation significantly increased the levels of KGF in the injured 
      liver, seemingly through suppression of miR-199, which targeted 3'-UTR of KGF 
      mRNA to inhibit its protein translation. CONCLUSIONS: HUCMSCs may ameliorate 
      cirrhosis through activation of KGF by suppressing miR-199.
FAU - Bi, Z-M
AU  - Bi ZM
AD  - Department of Hematology, No. 1 Hospital of Zibo Municipality, Zibo, China. 
      bzm6677@163.com.
FAU - Zhou, Q-F
AU  - Zhou QF
FAU - Geng, Y
AU  - Geng Y
FAU - Zhang, H-M
AU  - Zhang HM
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MicroRNAs)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
SB  - IM
MH  - Animals
MH  - Fibroblast Growth Factor 7/genetics
MH  - Humans
MH  - Liver Cirrhosis/genetics
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - MicroRNAs/*metabolism
MH  - Umbilical Cord/cytology
EDAT- 2016/12/17 06:00
MHDA- 2017/06/27 06:00
CRDT- 2016/12/17 06:00
PHST- 2016/12/17 06:00 [entrez]
PHST- 2016/12/17 06:00 [pubmed]
PHST- 2017/06/27 06:00 [medline]
AID - 11855 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2016 Dec;20(23):4905-4912.