PMID- 27983809 OWN - NLM STAT- MEDLINE DCOM- 20170914 LR - 20220107 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 65 IP - 33 DP - 2017 Aug 23 TI - A Collaborative Study: Determination of Mycotoxins in Corn, Peanut Butter, and Wheat Flour Using Stable Isotope Dilution Assay (SIDA) and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). PG - 7138-7152 LID - 10.1021/acs.jafc.6b04872 [doi] AB - A collaborative study was conducted to evaluate stable isotope dilution assay (SIDA) and LC-MS/MS for the simultaneous determination of aflatoxins B(1), B(2), G(1), and G(2); deoxynivalenol; fumonisins B(1), B(2), and B(3); ochratoxin A; HT-2 toxin; T-2 toxin; and zearalenone in foods. Samples were fortified with 12 (13)C uniformly labeled mycotoxins ((13)C-IS) corresponding to the native mycotoxins and extracted with acetonitrile/water (50:50 v/v), followed by centrifugation, filtration, and LC-MS/MS analysis. In addition to certified reference materials, the six participating laboratories analyzed corn, peanut butter, and wheat flour fortified with the 12 mycotoxins at concentrations ranging from 1.0 to 1000 ng/g. Using their available LC-MS/MS platform, each laboratory developed in-house instrumental conditions for analysis. The majority of recoveries ranged from 80 to 120% with relative standard derivations (RSDs) <20%. Greater than 90% of the average recoveries of the participating laboratories were in the range of 90-110%, with repeatability RSD(r) (within laboratory) < 10% and reproducibility RSD(R) (among laboratory) < 15%. All Z scores of the results of certified reference materials were between -2 and 2. Using (13)C-IS eliminated the need for matrix-matched calibration standards for quantitation, simplified sample preparation, and achieved simultaneous identification and quantitation of multiple mycotoxins in a simple LC-MS/MS procedure. FAU - Zhang, Kai AU - Zhang K AUID- ORCID: 0000-0001-5668-9458 AD - Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration , 5001 Campus Drive, College Park, Maryland 20740, United States. FAU - Schaab, Matthew R AU - Schaab MR AD - Arizona Department of Health Services , 250 North 17th Avenue, Phoenix, Arizona 85007, United States. FAU - Southwood, Gavin AU - Southwood G AD - Arizona Department of Health Services , 250 North 17th Avenue, Phoenix, Arizona 85007, United States. FAU - Tor, Elizabeth R AU - Tor ER AD - California Animal Health and Food Safety Laboratory System, Toxicology Laboratory, Univerisity of California-Davis , West Health Sciences Drive, Davis, California 95616, United States. FAU - Aston, Linda S AU - Aston LS AD - California Animal Health and Food Safety Laboratory System, Toxicology Laboratory, Univerisity of California-Davis , West Health Sciences Drive, Davis, California 95616, United States. FAU - Song, Wenlu AU - Song W AD - Food & Drug Laboratory Branch, California Department of Public Health , 850 Marina Bay Parkway, G365, Richmond, California 94804, United States. FAU - Eitzer, Brian AU - Eitzer B AD - Department of Analytical Chemistry, Connecticut Agricultural Experiment Station , 123 Huntington Street, New Haven, Connecticut 06511, United States. FAU - Majumdar, Sanghamitra AU - Majumdar S AD - Department of Analytical Chemistry, Connecticut Agricultural Experiment Station , 123 Huntington Street, New Haven, Connecticut 06511, United States. FAU - Lapainis, Theodore AU - Lapainis T AD - Pacific Regional Laboratory Northwest, U.S. Food and Drug Administration 22201 23rd Drive S.E., Bothell, Washington 98021, United States. FAU - Mai, Huy AU - Mai H AD - Kansas City Laboratory, U.S. Food and Drug Administration , 11510 West 80th Street, Lenexa, Kansas 66214, United States. FAU - Tran, Kevin AU - Tran K AD - Kansas City Laboratory, U.S. Food and Drug Administration , 11510 West 80th Street, Lenexa, Kansas 66214, United States. FAU - El-Demerdash, Aref AU - El-Demerdash A AD - Kansas City Laboratory, U.S. Food and Drug Administration , 11510 West 80th Street, Lenexa, Kansas 66214, United States. FAU - Vega, Victor AU - Vega V AD - Southeast Regional Laboratory, U.S. Food and Drug Administration , 60 8th Street, Atlanta, Georgia 30309, United States. FAU - Cai, Yanxuan AU - Cai Y AD - Office of Regulatory Affairs, U.S. Food and Drug Administration , 12420 Parklawn Drive Element Building, Rockville, Maryland 20857, United States. FAU - Wong, Jon W AU - Wong JW AD - Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration , 5001 Campus Drive, College Park, Maryland 20740, United States. FAU - Krynitsky, Alexandra J AU - Krynitsky AJ AD - Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration , 5001 Campus Drive, College Park, Maryland 20740, United States. FAU - Begley, Timothy H AU - Begley TH AD - Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration , 5001 Campus Drive, College Park, Maryland 20740, United States. LA - eng GR - U18 FD005523/FD/FDA HHS/United States PT - Evaluation Study PT - Journal Article DEP - 20170103 PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Mycotoxins) SB - IM MH - Arachis/*chemistry MH - Chromatography, High Pressure Liquid/*methods MH - Flour/*analysis MH - Food Contamination/*analysis MH - *Indicator Dilution Techniques MH - Mycotoxins/*analysis MH - Tandem Mass Spectrometry/*methods MH - Triticum/*chemistry MH - Zea mays/*chemistry OTO - NOTNLM OT - LC-MS/MS OT - mycotoxin OT - stable isotope dilution assay (SIDA) EDAT- 2016/12/17 06:00 MHDA- 2017/09/15 06:00 CRDT- 2016/12/17 06:00 PHST- 2016/12/17 06:00 [pubmed] PHST- 2017/09/15 06:00 [medline] PHST- 2016/12/17 06:00 [entrez] AID - 10.1021/acs.jafc.6b04872 [doi] PST - ppublish SO - J Agric Food Chem. 2017 Aug 23;65(33):7138-7152. doi: 10.1021/acs.jafc.6b04872. Epub 2017 Jan 3.