PMID- 27992968
OWN - NLM
STAT- MEDLINE
DCOM- 20171207
LR  - 20220321
IS  - 2047-2927 (Electronic)
IS  - 2047-2919 (Linking)
VI  - 5
IP  - 2
DP  - 2017 Mar
TI  - Establishment of in vitro model of erectile dysfunction for the study of 
      high-glucose-induced angiopathy and neuropathy.
PG  - 327-335
LID - 10.1111/andr.12307 [doi]
AB  - Penile erection requires complex interaction between vascular endothelial cells, 
      smooth muscle cells, pericytes, and autonomic nerves. Diabetes mellitus is one of 
      the most common causes of erectile dysfunction (ED) and multiple pathogenic 
      factors, such as cavernous angiopathy and autonomic neuropathy, are associated 
      with diabetic ED. Although a variety of animal models of diabetic ED play an 
      important role in understanding pathophysiologic mechanisms of diabetes-induced 
      ED, these animal models have limitations for addressing the exact cellular or 
      molecular mechanisms involved in ED. Therefore, we established an in vitro model 
      of ED for the study of high-glucose-induced angiopathy and neuropathy. We 
      successfully isolated and cultivated mouse cavernous endothelial cells (MCECs) 
      and mouse cavernous pericytes (MCPs). The cells were exposed to the 
      normal-glucose (5 mmoL) or high-glucose (30 mmoL) condition for 48 h. In vitro 
      matrigel assay revealed impairments in tube formation in primary cultured MCECs 
      or MCPs exposed to high-glucose condition. To study cellular interaction between 
      MCECs and MCPs, co-culture systems including indirect contact, indirect 
      non-contact, and direct mixed co-culture system, were established. We observed 
      impaired tube formation and increased permeability in MCECs-MCPs co-culture 
      exposed to high-glucose condition. To evaluate the effect of high-glucose on 
      neurite sprouting, the mouse major pelvic ganglion (MPG) tissue was harvested and 
      cultivated in matrigel. Neurite outgrowth and nNOS-positive nerve fibers were 
      significantly lower in MPG tissues exposed to the high-glucose condition than in 
      the tissues exposed to the normal-glucose condition. We believe that in vitro 
      model of ED will aid us to understand the role of each cellular component in the 
      pathogenesis of diabetic ED, and also be a useful tool for determining the 
      efficacy of candidate therapeutics targeting vascular or neuronal function. This 
      model would present a new avenue for drug discovery and development of novel 
      therapeutic modalities for erectile dysfunction.
CI  - (c) 2016 American Society of Andrology and European Academy of Andrology.
FAU - Yin, G N
AU  - Yin GN
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Park, S-H
AU  - Park SH
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Song, K-M
AU  - Song KM
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Limanjaya, A
AU  - Limanjaya A
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Ghatak, K
AU  - Ghatak K
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Minh, N N
AU  - Minh NN
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Ock, J
AU  - Ock J
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Ryu, J-K
AU  - Ryu JK
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
AD  - Inha Research Institute for Medical Sciences, Inha University School of Medicine, 
      Incheon, Korea.
FAU - Suh, J-K
AU  - Suh JK
AD  - National Research Center for Sexual Medicine and Department of Urology, Inha 
      University School of Medicine, Incheon, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161219
PL  - England
TA  - Andrology
JT  - Andrology
JID - 101585129
RN  - 0 (Drug Combinations)
RN  - 0 (Laminin)
RN  - 0 (Proteoglycans)
RN  - 119978-18-6 (matrigel)
RN  - 9007-34-5 (Collagen)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Coculture Techniques
MH  - Collagen
MH  - Diabetic Angiopathies/*physiopathology
MH  - Diabetic Neuropathies/*physiopathology
MH  - Disease Models, Animal
MH  - Drug Combinations
MH  - Endothelial Cells/*drug effects
MH  - Erectile Dysfunction/*physiopathology
MH  - Glucose/*pharmacology
MH  - Laminin
MH  - Male
MH  - Mice
MH  - Pericytes/*drug effects
MH  - Permeability
MH  - Proteoglycans
OTO - NOTNLM
OT  - angiopathy
OT  - erectile dysfunction
OT  - neuropathy
EDAT- 2016/12/20 06:00
MHDA- 2017/12/08 06:00
CRDT- 2016/12/20 06:00
PHST- 2016/06/29 00:00 [received]
PHST- 2016/10/16 00:00 [revised]
PHST- 2016/11/02 00:00 [accepted]
PHST- 2016/12/20 06:00 [pubmed]
PHST- 2017/12/08 06:00 [medline]
PHST- 2016/12/20 06:00 [entrez]
AID - 10.1111/andr.12307 [doi]
PST - ppublish
SO  - Andrology. 2017 Mar;5(2):327-335. doi: 10.1111/andr.12307. Epub 2016 Dec 19.