PMID- 27995148
OWN - NLM
STAT- MEDLINE
DCOM- 20170606
LR  - 20190723
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2016
DP  - 2016
TI  - Investigation of the Effects and Mechanisms of Mai Tong Formula on Lower Limb 
      Macroangiopathy in a Spontaneous Diabetic Rat Model.
PG  - 8076796
LID - 8076796
AB  - A new Chinese herbal formula called Mai Tong Formulae (MTF) has recently been 
      used to treat lower limb macroangiopathy in type 2 diabetes mellitus (T2DM) 
      patients. In this study, we investigated the effect of MTF on lower limb 
      macroangiopathy in a spontaneous diabetic rat model (GK rats). We found that MTF 
      treatment significantly reduced serum fasting blood glucose (FBG), triglycerides 
      (TG), total cholesterol (TC), IL6, and VEGF and increased serum insulin in this 
      model. Histological and ultrastructural observations showed that MTF treatment 
      significantly reduced vascular endothelial cell shedding and improved endothelium 
      injuries. We further detect proteome alteration following MTF treatment. 25 
      differential proteins (DPs) abnormally expressed in GK rats were normalized by 
      MTF treatment. These DPs significantly are enriched in biological processes and 
      pathways that regulate muscle contraction and cGMP-PKG signaling pathway and so 
      on. Additional protein-protein interaction (PPI) network analyses of the DPs 
      showed that Fasn and Prkar2a are involved in the AMPK signaling pathway, and 
      Gnas, Myh11, and Myh6 are involved in vascular smooth muscle contraction; these 5 
      DPs were validated by Western blotting. These results indicate that MTF treatment 
      effectively treats lower limb macroangiopathy by regulating key proteins involved 
      in AMPK signaling pathway and vascular smooth muscle contraction.
FAU - Gong, Guangming
AU  - Gong G
AUID- ORCID: 0000-0001-9107-1055
AD  - Endocrinology Department, Teaching Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu 610075, China.
FAU - Yuan, Haipo
AU  - Yuan H
AD  - Endocrinology Department, Teaching Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu 610075, China.
FAU - Liu, Ya
AU  - Liu Y
AD  - Endocrinology Department, Teaching Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu 610075, China.
FAU - Qi, Luguang
AU  - Qi L
AD  - Endocrinology Department, Teaching Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu 610075, China.
LA  - eng
PT  - Journal Article
DEP - 20161122
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (Blood Glucose)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Animals, Inbred Strains
MH  - Blood Glucose/drug effects/metabolism
MH  - Blotting, Western
MH  - Cholesterol/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Diabetes Mellitus/*metabolism
MH  - Diabetic Angiopathies/*metabolism/pathology
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Female
MH  - Femoral Artery/*drug effects/metabolism/pathology/ultrastructure
MH  - Lower Extremity/blood supply
MH  - Proteomics
MH  - Rats
MH  - Triglycerides/metabolism
PMC - PMC5138487
COIS- The authors declare that they have no competing interests.
EDAT- 2016/12/21 06:00
MHDA- 2017/06/07 06:00
PMCR- 2016/11/22
CRDT- 2016/12/21 06:00
PHST- 2016/09/20 00:00 [received]
PHST- 2016/10/19 00:00 [accepted]
PHST- 2016/12/21 06:00 [entrez]
PHST- 2016/12/21 06:00 [pubmed]
PHST- 2017/06/07 06:00 [medline]
PHST- 2016/11/22 00:00 [pmc-release]
AID - 10.1155/2016/8076796 [doi]
PST - ppublish
SO  - J Diabetes Res. 2016;2016:8076796. doi: 10.1155/2016/8076796. Epub 2016 Nov 22.