PMID- 27997269
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20220331
IS  - 1537-6524 (Electronic)
IS  - 1537-6516 (Linking)
VI  - 27
IP  - 3
DP  - 2017 Mar
TI  - The effects of endoplasmic reticulum stress inducer thapsigargin on the toxicity 
      of ZnO or TiO(2) nanoparticles to human endothelial cells.
PG  - 191-200
LID - 10.1080/15376516.2016.1273429 [doi]
AB  - It was recently shown that ZnO nanoparticles (NPs) could induce endoplasmic 
      reticulum (ER) stress in human umbilical vein endothelial cells (HUVECs). If ER 
      stress is associated the toxicity of ZnO NPs, the presence of ER stress inducer 
      thapsigargin (TG) should alter the response of HUVECs to ZnO NP exposure. In this 
      study, we addressed this issue by assessing cytotoxicity, oxidative stress and 
      inflammatory responses in ZnO NP exposed HUVECs with or without the presence of 
      TG. Moreover, TiO(2) NPs were used to compare the effects. Exposure to 32 mug/mL 
      ZnO NPs (p < 0.05), but not TiO(2) NPs (p > 0.05), significantly induced 
      cytotoxicity as assessed by WST-1 and neutral red uptake assay, as well as 
      intracellular ROS. ZnO NPs dose-dependently increased the accumulation of 
      intracellular Zn ions, and ZnSO(4) induced similar cytotoxic effects as ZnO NPs, 
      which indicated a role of Zn ions. The release of inflammatory proteins tumor 
      necrosis factor alpha (TNFalpha) and interleukin-6 (IL-6) or the adhesion of THP-1 
      monocytes to HUVECs was not significantly affected by ZnO or TiO(2) NP exposure 
      (p > 0.05). The presence of 250 nM TG significantly induced cytotoxicity, release 
      of IL-6 and THP-1 monocyte adhesion (p < 0.01), but did not significantly affect 
      intracellular ROS or release of TNFalpha (p > 0.05). ANOVA analysis indicated no 
      interaction between exposure to ZnO NPs and the presence of TG on almost all the 
      endpoints (p > 0.05) except neutral red uptake assay (p < 0.01). We concluded ER 
      stress is probably not associated with ZnO NP exposure induced oxidative stress 
      and inflammatory responses in HUVECs.
FAU - Gu, Yuxiu
AU  - Gu Y
AD  - a Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Laboratory of Biochemistry , College of Chemistry, Xiangtan University 
      , Xiangtan , PR China.
FAU - Cheng, Shanshan
AU  - Cheng S
AD  - a Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Laboratory of Biochemistry , College of Chemistry, Xiangtan University 
      , Xiangtan , PR China.
FAU - Chen, Gui
AU  - Chen G
AD  - a Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Laboratory of Biochemistry , College of Chemistry, Xiangtan University 
      , Xiangtan , PR China.
FAU - Shen, Yuexin
AU  - Shen Y
AD  - a Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Laboratory of Biochemistry , College of Chemistry, Xiangtan University 
      , Xiangtan , PR China.
FAU - Li, Xiyue
AU  - Li X
AD  - a Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Laboratory of Biochemistry , College of Chemistry, Xiangtan University 
      , Xiangtan , PR China.
FAU - Jiang, Qin
AU  - Jiang Q
AD  - a Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Laboratory of Biochemistry , College of Chemistry, Xiangtan University 
      , Xiangtan , PR China.
FAU - Li, Juan
AU  - Li J
AD  - a Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Laboratory of Biochemistry , College of Chemistry, Xiangtan University 
      , Xiangtan , PR China.
FAU - Cao, Yi
AU  - Cao Y
AD  - a Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of 
      Education, Laboratory of Biochemistry , College of Chemistry, Xiangtan University 
      , Xiangtan , PR China.
LA  - eng
PT  - Journal Article
DEP - 20170108
PL  - England
TA  - Toxicol Mech Methods
JT  - Toxicology mechanisms and methods
JID - 101134521
RN  - 0 (Reactive Oxygen Species)
RN  - 15FIX9V2JP (titanium dioxide)
RN  - 67526-95-8 (Thapsigargin)
RN  - D1JT611TNE (Titanium)
RN  - SOI2LOH54Z (Zinc Oxide)
SB  - IM
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/metabolism/ultrastructure
MH  - Humans
MH  - Nanoparticles/chemistry/*toxicity
MH  - Oxidative Stress/drug effects
MH  - Particle Size
MH  - Reactive Oxygen Species/metabolism
MH  - Surface Properties
MH  - Thapsigargin/chemistry/*toxicity
MH  - Titanium/chemistry/*toxicity
MH  - Zinc Oxide/chemistry/*toxicity
OTO - NOTNLM
OT  - TiO2 nanoparticles
OT  - ZnO nanoparticles
OT  - endoplasmic reticulum stress
OT  - human umbilical vein endothelial cells
OT  - thapsigargin
EDAT- 2016/12/21 06:00
MHDA- 2017/07/27 06:00
CRDT- 2016/12/21 06:00
PHST- 2016/12/21 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
PHST- 2016/12/21 06:00 [entrez]
AID - 10.1080/15376516.2016.1273429 [doi]
PST - ppublish
SO  - Toxicol Mech Methods. 2017 Mar;27(3):191-200. doi: 10.1080/15376516.2016.1273429. 
      Epub 2017 Jan 8.