PMID- 27998072
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20170605
IS  - 1520-4812 (Electronic)
IS  - 1043-1802 (Linking)
VI  - 27
IP  - 12
DP  - 2016 Dec 21
TI  - Effect of Several HIV Antigens Simultaneously Loaded with G2-NN16 Carbosilane 
      Dendrimer in the Cell Uptake and Functionality of Human Dendritic Cells.
PG  - 2844-2849
LID - 10.1021/acs.bioconjchem.6b00623 [doi]
AB  - Dendrimers are highly branched, star-shaped, and nanosized polymers that have 
      been proposed as new carriers for specific HIV-1 peptides. Dendritic cells (DCs) 
      are the most-potent antigen-presenting cells that play a major role in the 
      development of cell-mediated immunotherapy due to the generation and regulation 
      of adaptive immune responses against HIV-1. This article reports on the 
      associated behavior of two or three HIV-derived peptides simultaneously 
      (p24/gp160 or p24/gp160/NEF) with cationic carbosilane dendrimer G2-NN16. We have 
      found that (i) immature DCs (iDCs) and mature (mDCs) did not capture efficiently 
      HIV peptides regarding the uptake level when cells were treated with 
      G2-NN16-peptide complex alone; (ii) the ability of DCs to migrate was not 
      depending on the peptides presence; and (iii) with the use of molecular dynamic 
      simulation, a mixture of peptides decreased the cell uptake of the other peptides 
      (in particular, NEF hinders the binding of more peptides and is especially 
      obstructing of the binding of gp160 to G2-NN16). The results suggest that G2-NN16 
      cannot be considered as an alternative carrier for delivering two or more 
      HIV-derived peptides to DCs.
FAU - Sepulveda-Crespo, Daniel
AU  - Sepulveda-Crespo D
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon (IiSGM), Spanish HIV-HGM 
      BioBank , Madrid 28007, Spain.
AD  - Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine 
      (CIBER-BBN) , Madrid 28029, Spain.
FAU - Vacas-Cordoba, Enrique
AU  - Vacas-Cordoba E
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon (IiSGM), Spanish HIV-HGM 
      BioBank , Madrid 28007, Spain.
AD  - Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine 
      (CIBER-BBN) , Madrid 28029, Spain.
FAU - Marquez-Miranda, Valeria
AU  - Marquez-Miranda V
AD  - Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Biologia, 
      Universidad Andres Bello , Av. Republica 239, Santiago 8320000, Chile.
AD  - Fundacion Fraunhofer Chile Research , Las Condes 7550296, Chile.
FAU - Araya-Duran, Ingrid
AU  - Araya-Duran I
AD  - Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Biologia, 
      Universidad Andres Bello , Av. Republica 239, Santiago 8320000, Chile.
AD  - Fundacion Fraunhofer Chile Research , Las Condes 7550296, Chile.
FAU - Gomez, Rafael
AU  - Gomez R
AD  - Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine 
      (CIBER-BBN) , Madrid 28029, Spain.
AD  - Departamento Quimica Organica y Quimica Inorganica, Universidad de Alcala 
      Henares, Campus Universitario , Alcala de Henares 28805, Spain.
FAU - Mata, Francisco Javier de la
AU  - Mata FJ
AD  - Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine 
      (CIBER-BBN) , Madrid 28029, Spain.
AD  - Departamento Quimica Organica y Quimica Inorganica, Universidad de Alcala 
      Henares, Campus Universitario , Alcala de Henares 28805, Spain.
FAU - Gonzalez-Nilo, Fernando Danilo
AU  - Gonzalez-Nilo FD
AD  - Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Biologia, 
      Universidad Andres Bello , Av. Republica 239, Santiago 8320000, Chile.
AD  - Fundacion Fraunhofer Chile Research , Las Condes 7550296, Chile.
AD  - Centro Interdisciplinario de Neurociencia de Valparaiso, Facultad de Ciencias, 
      Universidad de Valparaiso , Valparaiso 8370071, Chile.
FAU - Munoz-Fernandez, Ma Angeles
AU  - Munoz-Fernandez MA
AUID- ORCID: 0000-0002-0813-4500
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon (IiSGM), Spanish HIV-HGM 
      BioBank , Madrid 28007, Spain.
AD  - Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine 
      (CIBER-BBN) , Madrid 28029, Spain.
LA  - eng
PT  - Journal Article
DEP - 20161201
PL  - United States
TA  - Bioconjug Chem
JT  - Bioconjugate chemistry
JID - 9010319
RN  - 0 (2G-NN16)
RN  - 0 (Dendrimers)
RN  - 0 (HIV Antigens)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (HIV Envelope Protein gp160)
RN  - 0 (Silanes)
RN  - 0 (carbosilane)
SB  - IM
MH  - Dendrimers/*chemistry/pharmacokinetics
MH  - Dendritic Cells/*drug effects
MH  - HIV Antigens/*chemistry/pharmacology
MH  - HIV Core Protein p24/chemistry
MH  - HIV Envelope Protein gp160/chemistry
MH  - Humans
MH  - Molecular Dynamics Simulation
MH  - Silanes/*chemistry/pharmacokinetics
MH  - Static Electricity
EDAT- 2016/12/22 06:00
MHDA- 2017/06/06 06:00
CRDT- 2016/12/22 06:00
PHST- 2016/12/22 06:00 [entrez]
PHST- 2016/12/22 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
AID - 10.1021/acs.bioconjchem.6b00623 [doi]
PST - ppublish
SO  - Bioconjug Chem. 2016 Dec 21;27(12):2844-2849. doi: 
      10.1021/acs.bioconjchem.6b00623. Epub 2016 Dec 1.