PMID- 28002028
OWN - NLM
STAT- MEDLINE
DCOM- 20170731
LR  - 20201216
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 55
IP  - 7
DP  - 2017 Jun 27
TI  - Within-day biological variation and hour-to-hour reference change values for 
      hematological parameters.
PG  - 1013-1024
LID - 10.1515/cclm-2016-0716 [doi]
AB  - BACKGROUND: Middle- and long-term biological variation data for hematological 
      parameters have been reported in the literature. Within-day 24-h variability 
      profiles for hematological parameters are currently lacking. However, 
      comprehensive hour-to-hour variability data are critical to detect diurnal 
      cyclical rhythms, and to take into account the 'time of sample collection' as a 
      possible determinant of natural fluctuation. In this study, we assessed 24-h 
      variation profiles for 20 hematological parameters. METHODS: Blood samples were 
      collected under standardized conditions from 24 subjects every hour for 24 h. At 
      each measurement, 20 hematological parameters were determined in duplicate. 
      Analytical variation (CVA), within-subject biological variation (CVI), 
      between-subject biological variation (CVG), index of individuality (II), and 
      reference change values (RCVs) were calculated. For the parameters with a diurnal 
      rhythm, hour-to-hour RCVs were determined. RESULTS: All parameters showed higher 
      CVG than CVI. Highest CVG was found for eosinophils (46.6%; 95% CI, 34.9%-70.1%) 
      and the lowest value was mean corpuscular hemoglobin concentration (MCHC) (3.2%; 
      95% CI, 2.4%-4.8%). CVI varied from 0.4% (95% CI, 0.32%-0.42%) to 20.9% (95% CI, 
      19.4%-22.6%) for red cell distribution width (RDW) and eosinophils, respectively. 
      Six hematological parameters showed a diurnal rhythm. CONCLUSIONS: We present 
      complete 24-h variability profiles for 20 hematological parameters. Hour-to-hour 
      reference changes values may help to better discriminate between random 
      fluctuations and true changes in parameters with rhythmic diurnal oscillations.
FAU - Hilderink, Judith M
AU  - Hilderink JM
AD  - .
FAU - Klinkenberg, Lieke J J
AU  - Klinkenberg LJJ
AD  - .
FAU - Aakre, Kristin M
AU  - Aakre KM
AD  - .
FAU - de Wit, Norbert C J
AU  - de Wit NCJ
AD  - .
FAU - Henskens, Yvonne M C
AU  - Henskens YMC
AD  - .
FAU - van der Linden, Noreen
AU  - van der Linden N
AD  - .
FAU - Bekers, Otto
AU  - Bekers O
AD  - .
FAU - Rennenberg, Roger J M W
AU  - Rennenberg RJMW
AD  - .
FAU - Koopmans, Richard P
AU  - Koopmans RP
AD  - .
FAU - Meex, Steven J R
AU  - Meex SJR
AD  - .
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - *Circadian Rhythm
MH  - Female
MH  - Hematologic Tests/*standards
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Posture
MH  - Reference Values
MH  - Specimen Handling/*methods
OTO - NOTNLM
OT  - biological variation
OT  - diurnal rhythm
OT  - hematology
OT  - reference change value
EDAT- 2016/12/22 06:00
MHDA- 2017/08/02 06:00
CRDT- 2016/12/22 06:00
PHST- 2016/08/10 00:00 [received]
PHST- 2016/11/01 00:00 [accepted]
PHST- 2016/12/22 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
PHST- 2016/12/22 06:00 [entrez]
AID - /j/cclm.ahead-of-print/cclm-2016-0716/cclm-2016-0716.xml [pii]
AID - 10.1515/cclm-2016-0716 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2017 Jun 27;55(7):1013-1024. doi: 10.1515/cclm-2016-0716.