PMID- 28002819 OWN - NLM STAT- MEDLINE DCOM- 20170221 LR - 20170221 IS - 1424-859X (Electronic) IS - 1424-8581 (Linking) VI - 150 IP - 2 DP - 2016 TI - Divergence between C. melo and African Cucumis Species Identified by Chromosome Painting and rDNA Distribution Pattern. PG - 150-155 LID - 10.1159/000453520 [doi] AB - The 5S and 45S rDNA sites are useful chromosome landmarks and can provide valuable information about karyotype evolution and species interrelationships. In this study, we employed fluorescence in situ hybridization (FISH) to determine the number and chromosomal location of 5S and 45S rDNA loci in 8 diploid Cucumis species. Two oligonucleotide painting probes specific for the rDNA-bearing chromosomes in C. melo were hybridized to other Cucumis species in order to investigate the homeologies among the rDNA-carrying chromosomes in Cucumis species. The analyzed diploid species showed 3 types of rDNA distribution patterns, which provided clear cytogenetic evidence on the divergence between C. melo and wild diploid African Cucumis species. The present results not only show species interrelationships in the genus Cucumis, but the rDNA FISH patterns can also be used as cytological markers for the discrimination of closely related species. The data will be helpful for breeders to choose the most suitable species from various wild species for improvement of cultivated melon. CI - (c) 2016 S. Karger AG, Basel. FAU - Li, Kunpeng AU - Li K AD - School of Life Sciences, Jiangsu Normal University, Xuzhou, China. FAU - Wang, Huaisong AU - Wang H FAU - Wang, Jiming AU - Wang J FAU - Sun, Jianying AU - Sun J FAU - Li, Zongyun AU - Li Z FAU - Han, Yonghua AU - Han Y LA - eng PT - Comparative Study PT - Journal Article DEP - 20161222 PL - Switzerland TA - Cytogenet Genome Res JT - Cytogenetic and genome research JID - 101142708 RN - 0 (DNA Probes) RN - 0 (DNA, Plant) RN - 0 (DNA, Ribosomal) SB - IM MH - Africa MH - Chromosome Painting MH - Chromosomes, Plant/genetics MH - Cucumis/classification/*genetics MH - DNA Probes MH - DNA, Plant/genetics MH - DNA, Ribosomal/genetics MH - Diploidy MH - In Situ Hybridization, Fluorescence MH - Phylogeny MH - Species Specificity EDAT- 2016/12/22 06:00 MHDA- 2017/02/22 06:00 CRDT- 2016/12/22 06:00 PHST- 2016/08/16 00:00 [accepted] PHST- 2016/12/22 06:00 [pubmed] PHST- 2017/02/22 06:00 [medline] PHST- 2016/12/22 06:00 [entrez] AID - 000453520 [pii] AID - 10.1159/000453520 [doi] PST - ppublish SO - Cytogenet Genome Res. 2016;150(2):150-155. doi: 10.1159/000453520. Epub 2016 Dec 22.