PMID- 28003579
OWN - NLM
STAT- MEDLINE
DCOM- 20170118
LR  - 20170118
IS  - 1880-313X (Electronic)
IS  - 0388-6107 (Linking)
VI  - 37
IP  - 6
DP  - 2016
TI  - Effect of 1,25-dihydroxyvitamin D(3) on spontaneous calcium responses in rat 
      dental epithelial SF2 cells revealed by long-term imaging.
PG  - 329-334
LID - 10.2220/biomedres.37.329 [doi]
AB  - Genetically encoded calcium indicators (GECIs) are suitable for long-term imaging 
      studies. In this study, we employed a highly sensitive GECI, G-GECO, and achieved 
      efficient gene delivery with an adenoviral vector. The adenoviral vector allowed 
      us to express G-GECO in more than 80% of cells. More than 80% of 
      G-GECO-expressing cells showed an ATP-induced increase in fluorescence intensity 
      due to Ca(2+) release from intracellular stores and subsequent Ca(2+) entry. The 
      fluorescence intensity of these cells was increased more than 2-fold by 
      stimulation with 10 muM ATP. We applied long-term imaging (for ~10 h) to monitor 
      Ca(2+) responses in SF2, a rat dental epithelial cell line, in culture 
      conditions. SF2 cells showed intermittent rises in the intracellular Ca(2+) 
      concentration in the presence of 100 nM 1,25-dihydroxyvitamin D(3). Many of these 
      Ca(2+) responses began at a specific location in the cytoplasm and spread 
      throughout the entire cytoplasm. The combination of efficient gene delivery with 
      an adenoviral vector and long-term imaging with a highly sensitive GECI enabled 
      detection of intermittent Ca(2+) responses that occur only 3-10 times/h/100 
      cells. This method could be useful to study the effects of Ca(2+) responses for 
      regulating longterm processes, such as gene expression, cell migration, and cell 
      division, in many cell types.
FAU - Murata, Kaori
AU  - Murata K
AD  - Department of Pediatric Dentistry, School of Dentistry, Health Sciences 
      University of Hokkaido.
FAU - Takahashi, Ayumi
AU  - Takahashi A
FAU - Morita, Takao
AU  - Morita T
FAU - Nezu, Akihiro
AU  - Nezu A
FAU - Fukumoto, Satoshi
AU  - Fukumoto S
FAU - Saitoh, Masato
AU  - Saitoh M
FAU - Tanimura, Akihiro
AU  - Tanimura A
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biomed Res
JT  - Biomedical research (Tokyo, Japan)
JID - 8100317
RN  - 1406-16-2 (Vitamin D)
RN  - 66772-14-3 (1,25-dihydroxyvitamin D)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Signaling/*drug effects
MH  - Cell Line
MH  - Epithelial Cells/*drug effects/*metabolism
MH  - Gene Expression
MH  - Genes, Reporter
MH  - Humans
MH  - *Molecular Imaging
MH  - Rats
MH  - Vitamin D/*analogs & derivatives/pharmacology
EDAT- 2016/12/23 06:00
MHDA- 2017/01/19 06:00
CRDT- 2016/12/23 06:00
PHST- 2016/12/23 06:00 [entrez]
PHST- 2016/12/23 06:00 [pubmed]
PHST- 2017/01/19 06:00 [medline]
AID - 10.2220/biomedres.37.329 [doi]
PST - ppublish
SO  - Biomed Res. 2016;37(6):329-334. doi: 10.2220/biomedres.37.329.